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AQP5 elisa kit :: Mouse Aquaporin 5 (AQP5) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2021144 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Typical Testing Data/Standard Curve
Unit / Price
24-Strip-Wells  /  $260 +1 FREE 8GB USB
48-Strip-Wells  /  $465 +1 FREE 8GB USB
96-Strip-Wells  /  $625 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,530 +3 FREE 8GB USB
10x96-Strip-Wells  /  $4,565 +5 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Aquaporin 5 (AQP5), ELISA Kit

★Popular Item★
 Also Known As   

Aquaporin 5 (AQP5) ELISA Kit

 Product Gene Name   

AQP5 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    600231
 3D Structure    ModBase 3D Structure for P55064
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 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Aquaporin 5 (AQP5).
No significant cross-reactivity or interference between Aquaporin 5 (AQP5) and analogues was observed.
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 Assay Type    Double-antibody Sandwich
 Samples    Saliva, Tissue homogenates, Cell lysates and Other Biological Fluids
 Detection Range    0.156-10ng/mL
 Sensitivity    < 0.057ng/mL
 Application    Enzyme-linked immunosorbent assay for Antigen Detection.
 Intra-assay Precision (Precision within an assay)    3 samples with low, middle and high level Aquaporin 5 (AQP5) were tested 20 times on one plate, respectively.
 Inter-assay Precision (Precision between assays)    3 samples with low, middle and high level Aquaporin 5 (AQP5) were tested on 3 different plates, 8 replicates in each plate.
 CV(%) =    SD/meanX100
 Intra-Assay    CV<10%
 Inter-Assay    CV<12%
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 Preparation and Storage    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of AQP5 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for AQP5 purchase    MBS2021144 is a ready-to-use microwell, strip plate Double-antibody Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Aquaporin 5 (AQP5) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.156-10ng/mL in biological research samples containing AQP5, with an estimated sensitivity of < 0.057ng/mL. The ELISA analytical biochemical technique of the MBS2021144 kit is based on AQP5 antibody-AQP5 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect AQP5 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, AQP5. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Saliva, Tissue homogenates, Cell lysates and Other Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for AQP5 elisa kit

   The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Aquaporin 5 (AQP5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Aquaporin 5 (AQP5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Aquaporin 5 (AQP5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Aquaporin 5 (AQP5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Product Categories/Family for AQP5 elisa kit    Metabolic pathway; Infection immunity; Pulmonology
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 Typical Testing Data/Standard Curve of AQP5 elisa kit    AQP5 elisa kit Typical Testing Data/Standard Curve image
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Sample Manual Insert of MBS2021144. Click to request current manual
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NCBI/Uniprot data below describe general gene information for AQP5. It may not necessarily be applicable to this product.
 NCBI GI #    119578514
 NCBI GeneID    362
 NCBI Accession #    EAW58110.1 [Other Products]
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 UniProt Primary Accession #    P55064 [Other Products]
 UniProt Secondary Accession #    Q6FGW8 [Other Products]
 UniProt Related Accession #    P55064 [Other Products]
 Molecular Weight    28,292 Da
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 NCBI Official Full Name    aquaporin 5
 NCBI Official Synonym Full Names    aquaporin 5
 NCBI Official Symbol    AQP5 [Similar Products]
 NCBI Official Synonym Symbols   
PPKB; AQP-5
[Similar Products]
 NCBI Protein Information    aquaporin-5
 UniProt Protein Name    Aquaporin-5
 Protein Family    Aquaporin
 UniProt Gene Name    AQP5 [Similar Products]
 UniProt Synonym Gene Names    AQP-5 [Similar Products]
 UniProt Entry Name    AQP5_HUMAN
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 NCBI Summary for AQP5    Aquaporin 5 (AQP5) is a water channel protein. Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein (MIP or AQP0). Aquaporin 5 plays a role in the generation of saliva, tears and pulmonary secretions. AQP0, AQP2, AQP5, and AQP6 are closely related and all map to 12q13. [provided by RefSeq, Jul 2008]
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 UniProt Comments for AQP5    AQP5: Forms a water-specific channel. Implicated in the generation of saliva, tears, and pulmonary secretions. Belongs to the MIP/aquaporin (TC 1.A.8) family.

Protein type: Transporter, aquaporin family; Transporter; Membrane protein, integral; Membrane protein, multi-pass; Channel, misc.

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: microvillus; integral to plasma membrane; endoplasmic reticulum; apical plasma membrane; basal plasma membrane; plasma membrane

Molecular Function: protein binding; water channel activity; glycerol channel activity

Biological Process: odontogenesis; glycerol transport; cellular water homeostasis; camera-type eye morphogenesis; water transport; carbon dioxide transport; excretion; pancreatic juice secretion; transmembrane transport; saliva secretion
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 Research Articles on AQP5    1. Study found a significant correlation between AQP1, AQP3, and AQP5 overexpression and lymph node metastasis in patients with surgically resected colon cancer.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with AQP5 elisa kitPathways associated with AQP5 elisa kit
 Reference Product  PubMed Publications
 AQP11 elisa kit  >12 publications with AQP5 and AQP11
 FGF7 elisa kit  >4 publications with AQP5 and FGF7
 TRPV4 elisa kit  >4 publications with AQP5 and TRPV4
 SRC elisa kit  >2 publications with AQP5 and SRC
 Products by Pathway  Pathway Diagram
 Aquaporin-mediated Transport Pathway antibodies  Aquaporin-mediated Transport Pathway Diagram
 Passive Transport By Aquaporins Pathway antibodies  Passive Transport By Aquaporins Pathway Diagram
 Salivary Secretion Pathway antibodies  Salivary Secretion Pathway Diagram
 Salivary Secretion Pathway antibodies  Salivary Secretion Pathway Diagram
 Stabilization And Expansion Of The E-cadherin Adherens Junction Pathway antibodies  Stabilization And Expansion Of The E-cadherin Adherens Junction Pathway Diagram
 Transmembrane Transport Of Small Molecules Pathway antibodies  Transmembrane Transport Of Small Molecules Pathway Diagram
Diseases associated with AQP5 elisa kitOrgans/Tissues associated with AQP5 elisa kit
 Disease Name  Pubmed Publications
 Edema Antibodies  >56 publications with AQP5 and Edema
 Disease Models, Animal Antibodies  >42 publications with AQP5 and Disease Models, Animal
 Inflammation Antibodies  >31 publications with AQP5 and Inflammation
 Nervous System Diseases Antibodies  >13 publications with AQP5 and Nervous System Diseases
 Necrosis Antibodies  >12 publications with AQP5 and Necrosis
 Hyperplasia Antibodies  >10 publications with AQP5 and Hyperplasia
 Kidney Diseases Antibodies  >10 publications with AQP5 and Kidney Diseases
 Lung Neoplasms Antibodies  >9 publications with AQP5 and Lung Neoplasms
 Breast Neoplasms Antibodies  >5 publications with AQP5 and Breast Neoplasms
 Liver Diseases Antibodies  >4 publications with AQP5 and Liver Diseases
 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >155 publications with AQP5 and Lung
 Eye Antibodies  >59 publications with AQP5 and Eye
 Brain Antibodies  >35 publications with AQP5 and Brain
 Skin Antibodies  >23 publications with AQP5 and Skin
 Uterus Antibodies  >19 publications with AQP5 and Uterus
 Stomach Antibodies  >14 publications with AQP5 and Stomach
 Intestine Antibodies  >10 publications with AQP5 and Intestine
 Testis Antibodies  >8 publications with AQP5 and Testis
 Ovary Antibodies  >5 publications with AQP5 and Ovary
 Connective Tissue Antibodies  >4 publications with AQP5 and Connective Tissue
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