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ALOX-5 elisa kit :: Mouse Arachidonate-5-Lipoxygenase (ALOX-5) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS264026
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $290 +1 FREE 8GB USB
96-Strip-Wells  /  $450 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,755 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,150 +3 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Arachidonate-5-Lipoxygenase (ALOX-5), ELISA Kit

 Also Known As   

Mouse Arachidonate-5-Lipoxygenase (ALOX-5) ELISA Kit

 Product Gene Name   

ALOX-5 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Manual Insert    Download PDF Manual View PDF Manual
 Species Reactivity    Mouse
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 Specificity    No cross-reaction with other factors.
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 Samples    Mouse serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant ALOX-5 concentration
 Assay Type    Sandwich
 Detection Range    20 ng/ml-0.312 ng/ml
 Sensitivity    0.06 ng/ml.
 Intra-assay Precision    <= 8%
 Inter-assay Precision    <= 12%
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay.
 Other Notes    Small volumes of ALOX-5 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for ALOX-5 purchase    MBS264026 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Arachidonate-5-Lipoxygenase (ALOX-5) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 20 ng/ml-0.312 ng/ml in biological research samples containing ALOX-5, with an estimated sensitivity of 0.06 ng/ml. The ELISA analytical biochemical technique of the MBS264026 kit is based on ALOX-5 antibody-ALOX-5 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ALOX-5 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ALOX-5. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Mouse serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant ALOX-5 concentration. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for ALOX-5 elisa kit

   Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse ALOX-5 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
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 Typical Testing Data/Standard Curve (for reference only) of ALOX-5 elisa kit    ALOX-5 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS264026
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NCBI/Uniprot data below describe general gene information for ALOX-5. It may not necessarily be applicable to this product.
 NCBI GI #    528078462
 NCBI GeneID    101839970
 NCBI Accession #    NP_001268516.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001281587.1 [Other Products]
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 UniProt Related Accession #    P51399 [Other Products]
 Molecular Weight    77,873 Da
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 NCBI Official Full Name    arachidonate 5-lipoxygenase
 NCBI Official Symbol    Alox5 [Similar Products]
 NCBI Official Synonym Symbols   
5-LO
[Similar Products]
 NCBI Protein Information    arachidonate 5-lipoxygenase
 UniProt Protein Name    Arachidonate 5-lipoxygenase
 UniProt Gene Name    ALOX5 [Similar Products]
 UniProt Synonym Gene Names    5-LO; 5-lipoxygenase [Similar Products]
 UniProt Entry Name    LOX5_MESAU
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 UniProt Comments for ALOX-5    Catalyzes the first step in leukotriene biosynthesis, and thereby plays a role in inflammatory processes.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Diseases associated with ALOX-5 elisa kit
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >586 publications with ALOX-5 and Inflammation
 Asthma Antibodies  >251 publications with ALOX-5 and Asthma
 Lung Diseases Antibodies  >237 publications with ALOX-5 and Lung Diseases
 Atherosclerosis Antibodies  >123 publications with ALOX-5 and Atherosclerosis
 Necrosis Antibodies  >115 publications with ALOX-5 and Necrosis
 Edema Antibodies  >94 publications with ALOX-5 and Edema
 Heart Diseases Antibodies  >59 publications with ALOX-5 and Heart Diseases
 Neoplasms, Experimental Antibodies  >52 publications with ALOX-5 and Neoplasms, Experimental
 Pain Antibodies  >49 publications with ALOX-5 and Pain
 Reperfusion Injury Antibodies  >43 publications with ALOX-5 and Reperfusion Injury
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