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ASPN elisa kit :: Mouse Asporin ELISA Kit

Scan QR to view Datasheet Catalog #    MBS723258
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $455 +1 FREE 8GB USB
96-Strip-Wells  /  $660 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,490 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Asporin (ASPN), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Asporin ELISA Kit

 Product Gene Name   

ASPN elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 3D Structure    ModBase 3D Structure for Q5TBF2
 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of ASPN. No significant cross-reactivity or interference between ASPN and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ASPN and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Detection Range    0.5-10ng/mL
 Sensitivity    0.1ng/mL
 Intended Uses    This ASPN ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse ASPN. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of ASPN elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for ASPN purchase    MBS723258 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Asporin (ASPN) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.5-10ng/mL in biological research samples containing ASPN, with an estimated sensitivity of 0.1ng/mL. The ELISA analytical biochemical technique of the MBS723258 kit is based on ASPN antibody-ASPN antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ASPN antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ASPN. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for ASPN elisa kit

   Principle of the assay: ASPN ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for ASPN. Standards or samples are then added to the microtiter plate wells and ASPN if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of ASPN present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for ASPN are added to each well to "sandwich" the ASPN immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain ASPN and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ASPN concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for ASPN elisa kit    Signal Transduction
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 Typical Testing Data/Standard Curve (for reference only) of ASPN elisa kit    ASPN elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS723258. Click to request current manual
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NCBI/Uniprot data below describe general gene information for ASPN. It may not necessarily be applicable to this product.
 NCBI GI #    55957240
 NCBI Accession #    CAI16698.1 [Other Products]
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 UniProt Primary Accession #    Q5TBF2 [Other Products]
 Molecular Weight    27,749 Da
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 UniProt Protein Name    Asporin
 UniProt Synonym Protein Names   
Asporin; Asporin (LRR class 1), isoform CRA_b
 Protein Family    Asporin
 UniProt Gene Name    ASPN [Similar Products]
 UniProt Synonym Gene Names    hCG_1784540 [Similar Products]
 UniProt Entry Name    Q5TBF2_HUMAN
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Organs/Tissues associated with ASPN elisa kit
 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >35 publications with ASPN and Bone
 Mouth Antibodies  >8 publications with ASPN and Mouth
 Muscle Antibodies  >6 publications with ASPN and Muscle
 Brain Antibodies  >5 publications with ASPN and Brain
 Connective Tissue Antibodies  >5 publications with ASPN and Connective Tissue
 Embryonic Tissue Antibodies  >5 publications with ASPN and Embryonic Tissue
 Heart Antibodies  >4 publications with ASPN and Heart
 Skin Antibodies  >3 publications with ASPN and Skin
 Liver Antibodies  >3 publications with ASPN and Liver
 Vascular Antibodies  >3 publications with ASPN and Vascular
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