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Add To Compare List BAFF elisa kit :: Mouse B-Cell Activating Factor (BAFF) ELISA Kit Catalog # MBS452855 Unit / Price 48 Tests / $460 +1 FREE 8GB USB 96 Tests / $605 +1 FREE 8GB USB 5x96 Tests / $2,740 +3 FREE 8GB USB 10x96 Tests / $5,350 +6 FREE 8GB USB Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name B-Cell Activating Factor (BAFF), ELISA Kit Full Product Name Mouse B-Cell Activating Factor (BAFF) ELISA Kit Product Synonym Names CD257; TNFSF13B; BLYS; TALL1; THANK; TNFSF20; ZTNF4; B lymphocyte stimulator; Tumor Necrosis Factor Ligand Superfamily Member 13B; Dendritic cell-derived TNF-like Product Gene Name BAFF elisa kit[Similar Products] Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP Sample Manual Insert Download Sample PDF Manual View Sample PDF Manual 3D Structure ModBase 3D Structure for Q9WU72 Species Reactivity Mouse Specificity This assay has high sensitivity and excellent specificity for detection of BAFF. No significant cross-reactivity or interference between BAFF and analogues was observed. Samples Serum, plasma, tissue homogenates and other biological fluids. Assay Type Sandwich Detection Range 1.56-100pg/mL Sensitivity 0.55pg/mL Intra-assay Precision Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level BAFF were tested 20 times on one plate, respectively. Intra-Assay: CV<10% Inter-assay Precision Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level BAFF were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12% Preparation and Storage For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C. ISO Certification Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory. Product Note Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details. Other Notes Small volumes of BAFF elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. Searchable Terms for BAFF purchase MBS452855 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the B-Cell Activating Factor (BAFF) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing BAFF. The ELISA analytical biochemical technique of the MBS452855 kit is based on BAFF antibody-BAFF antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect BAFF antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, BAFF. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%). TOP Related Product Information for BAFF elisa kit Intended Uses: This kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of BAFF in mouse serum, plasma, tissue homogenates and other biological fluids.Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to BAFF. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to BAFF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain BAFF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of BAFF in the samples is then determined by comparing the O.D. of the samples to the standard curve. Typical Testing Data/Standard Curve (for reference only) of BAFF elisa kit TOP Sample Manual Insert of MBS452855. NCBI/Uniprot data below describe general gene information for BAFF. It may not necessarily be applicable to this product. NCBI GI # 15826858 NCBI GeneID 24099 NCBI Accession # NP_296371.1 [Other Products] NCBI GenBank Nucleotide # NM_033622.1 [Other Products] UniProt Primary Accession # Q9WU72 [Other Products] UniProt Secondary Accession # Q7TQ58[Other Products] UniProt Related Accession # Q9WU72[Other Products] Molecular Weight 32,166 Da TOP NCBI Official Full Name tumor necrosis factor ligand superfamily member 13B isoform 1 NCBI Official Synonym Full Names tumor necrosis factor (ligand) superfamily, member 13b NCBI Official Symbol Tnfsf13b [Similar Products] NCBI Official Synonym Symbols BAFF; BLyS; TALL1; THANK; zTNF4; TALL-1; Tnlg7a; TNFSF20; D8Ertd387e [Similar Products] NCBI Protein Information tumor necrosis factor ligand superfamily member 13B UniProt Protein Name Tumor necrosis factor ligand superfamily member 13B UniProt Synonym Protein Names B-cell-activating factor; BAFF; CD_antigen: CD257 UniProt Gene Name Tnfsf13b [Similar Products] UniProt Synonym Gene Names Baff [Similar Products] TOP TOP UniProt Comments for BAFF Cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B- and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response. Research Articles on BAFF 1. The Tnfas13b in the hippocampus in the prefrontal cortex of the DBA/2 J strain were up-regulated. TOP Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. Disclaimer While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information. 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