View our list of available Coronavirus (COVID-19, SARS-CoV-2, 2019-nCOV) antibodies, recombinant proteins, ELISA Kits... Options Full Datasheet Printer Friendly Datasheet Prepare for Printing Full Datasheet Printer Friendly Datasheet Prepare for Printing Have Questions? Ask Us! Request A Quote Today! Add To Compare List CD80 elisa kit :: Mouse B7-1/CD80 ELISA Kit Catalog # MBS175825 Unit / Price 96-Strip-Wells / $610 +1 FREE 8GB USB 5x96-Strip-Wells / $2,765 +3 FREE 8GB USB Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name B7-1/CD80, ELISA Kit Popular Item Full Product Name Mouse B7-1/CD80 PicoKine ELISA Kit Product Synonym Names T-lymphocyte activation antigen CD80; CD80 molecule; Activation B7-1 antigen; B7; BB1; CD80; CD80_HUMAN; CTLA-4 counter-receptor B7.1; T-lymphocyte activation antigen CD80 Product Gene Name CD80 elisa kit[Similar Products] Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP Sample Manual Insert Download Sample PDF Manual View Sample PDF Manual 3D Structure ModBase 3D Structure for Q00609 Species Reactivity Mouse Specificity Natural and recombinant mouse CD80 Samples Cell culture supernates and serum Assay Type Sandwich Detection Range 62.5pg/ml-4000pg/ml Sensitivity < 10pg/ml Intra-assay Precision Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Inter-assay Precision Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. Preparation and Storage Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.) Production Time Due to severe delay and raw materials shortage, production time may takes between 5-8 weeks. ISO Certification Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory. Product Note Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details. Other Notes Small volumes of CD80 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. Searchable Terms for CD80 purchase MBS175825 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the B7-1/CD80, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CD80. The ELISA analytical biochemical technique of the MBS175825 kit is based on CD80 antibody-CD80 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CD80 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CD80. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%). TOP Related Product Information for CD80 elisa kit Principle of the assay: mouse CD80 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CD80 has been precoated onto 96-well plates. Standards(NSO, D37-K245) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD80 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CD80 amount of sample captured in plate.Background: The protein CD80 (Cluster of Differentiation 80) is a molecule found on activated B cells and monocytes which provides a costimulatory signal necessary for T cell activation and survival. It is also known as B7.1. The cDNA for B7-1 predicts a type I membrane protein, i.e., one synthesized with a signal peptide that is cleaved upon translocation across the endoplasmic membrane. The protein is predicted to contain 2 extracellular domains structurally similar to those of Ig, a hydrophobic transmembrane region, and a short cytoplasmic domain[1].The CD80 and CD86 (601020) genes encode B7-1 and B7-2, respectively, which are structurally similar members of the immunoglobulin superfamily expressed on a variety of hematopoietic cell types.[2] stated that B7-1 and B7-2 provide a costimulatory signal to T cells by interacting with CD28 and CTLA4. The standard product used in this kit is recombinant B7-1,D37K245,which is composed of two single chains acids with the dipolymer. Typical Testing Data/Standard Curve (for reference only) of CD80 elisa kit Mouse B7-1/CD80 PicoKine ELISA Kit standard curve TOP Sample Manual Insert of MBS175825. NCBI/Uniprot data below describe general gene information for CD80. It may not necessarily be applicable to this product. NCBI GI # 461607 NCBI GeneID 12519 NCBI Accession # Q00609.1 [Other Products] UniProt Primary Accession # Q00609 [Other Products] UniProt Secondary Accession # Q61332[Other Products] UniProt Related Accession # Q00609[Other Products] Molecular Weight 24,051 Da TOP NCBI Official Full Name T-lymphocyte activation antigen CD80 NCBI Official Synonym Full Names CD80 antigen NCBI Official Symbol Cd80 [Similar Products] NCBI Official Synonym Symbols B71; Ly53; TSA1; Cd28l; Ly-53; MIC17 [Similar Products] NCBI Protein Information T-lymphocyte activation antigen CD80; B7 protein; activation B7-1 antigen UniProt Protein Name T-lymphocyte activation antigen CD80 UniProt Synonym Protein Names Activation B7-1 antigen; B7; CD_antigen: CD80 Protein Family T-lymphocyte activation antigen UniProt Gene Name Cd80 [Similar Products] UniProt Synonym Gene Names B7; B7 [Similar Products] UniProt Entry Name CD80_MOUSE TOP TOP UniProt Comments for CD80 CD80: Involved in the costimulatory signal essential for T- lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor.Protein type: Membrane protein, integral; Immunoglobulin superfamilyCellular Component: cell surface; membrane; integral to membrane; external side of plasma membraneMolecular Function: protein bindingBiological Process: positive regulation of peptidyl-tyrosine phosphorylation; T cell costimulation; positive regulation of alpha-beta T cell proliferation; positive regulation of T cell proliferation Research Articles on CD80 1. The need for additional immune suppression in the intestine reflects commensal microbe-driven T-cell activation through the accessory costimulation molecules ICOSL and OX40L in B7 deprived mice. TOP Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. Disclaimer While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information. TOP Pathways associated with CD80 elisa kit Products by Pathway Pathway Diagram Adaptive Immune System Pathway antibodies Adaptive Immune System Pathway Diagram Allograft Rejection Pathway antibodies Allograft Rejection Pathway Diagram Allograft Rejection Pathway antibodies Allograft Rejection Pathway Diagram Autoimmune Thyroid Disease Pathway antibodies Autoimmune Thyroid Disease Pathway Diagram Autoimmune Thyroid Disease Pathway antibodies Autoimmune Thyroid Disease Pathway Diagram CD28 Co-stimulation Pathway antibodies CD28 Co-stimulation Pathway Diagram CD28 Dependent PI3K/Akt Signaling Pathway antibodies CD28 Dependent PI3K/Akt Signaling Pathway Diagram CD28 Dependent Vav1 Pathway antibodies CD28 Dependent Vav1 Pathway Diagram CTLA4 Inhibitory Signaling Pathway antibodies CTLA4 Inhibitory Signaling Pathway Diagram Cell Adhesion Molecules (CAMs) Pathway antibodies Cell Adhesion Molecules (CAMs) Pathway Diagram Diseases associated with CD80 elisa kit Disease Name Pubmed Publications Necrosis Antibodies >1099 publications with CD80 and Necrosis Inflammation Antibodies >728 publications with CD80 and Inflammation Neoplasms, Experimental Antibodies >376 publications with CD80 and Neoplasms, Experimental Nervous System Diseases Antibodies >342 publications with CD80 and Nervous System Diseases Lung Diseases Antibodies >301 publications with CD80 and Lung Diseases Liver Diseases Antibodies >221 publications with CD80 and Liver Diseases Kidney Diseases Antibodies >149 publications with CD80 and Kidney Diseases Lung Neoplasms Antibodies >110 publications with CD80 and Lung Neoplasms Neoplasm Metastasis Antibodies >81 publications with CD80 and Neoplasm Metastasis Breast Neoplasms Antibodies >80 publications with CD80 and Breast Neoplasms Organs/Tissues associated with CD80 elisa kit Organ/Tissue Name Pubmed Publications Bone Marrow Antibodies >893 publications with CD80 and Bone Marrow Skin Antibodies >385 publications with CD80 and Skin Thymus Antibodies >177 publications with CD80 and Thymus Brain Antibodies >163 publications with CD80 and Brain Muscle Antibodies >82 publications with CD80 and Muscle Connective Tissue Antibodies >33 publications with CD80 and Connective Tissue TOP © 2006-24 MyBioSource.com. 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