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CD80 elisa kit :: Mouse B7-1/CD80 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS175825
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
96-Strip-Wells  /  $455 +1 FREE 8GB USB
 
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 Product Name   

B7-1/CD80, ELISA Kit

★Popular Item★
 Also Known As   

Mouse B7-1/CD80 PicoKine ELISA Kit

 Product Synonym Names    T-lymphocyte activation antigen CD80; CD80 molecule; Activation B7-1 antigen; B7; BB1; CD80; CD80_HUMAN; CTLA-4 counter-receptor B7.1; T-lymphocyte activation antigen CD80
 Product Gene Name   

CD80 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 3D Structure    ModBase 3D Structure for Q00609
 Species Reactivity    Mouse
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 Specificity    Natural and recombinant mouse CD80
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 Samples    Cell culture supernates and serum
 Assay Type    Sandwich
 Detection Range    62.5pg/ml-4000pg/ml
 Sensitivity    < 10pg/ml
 Intra-assay Precision    Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
 Inter-assay Precision    Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
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 Preparation and Storage    Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of CD80 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for CD80 purchase    MBS175825 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the B7-1/CD80, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 62.5pg/ml-4000pg/ml in biological research samples containing CD80, with an estimated sensitivity of < 10pg/ml. The ELISA analytical biochemical technique of the MBS175825 kit is based on CD80 antibody-CD80 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CD80 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CD80. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Cell culture supernates and serum. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for CD80 elisa kit

   Principle of the assay: mouse CD80 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CD80 has been precoated onto 96-well plates. Standards(NSO, D37-K245) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD80 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CD80 amount of sample captured in plate.
Background: The protein CD80 (Cluster of Differentiation 80) is a molecule found on activated B cells and monocytes which provides a costimulatory signal necessary for T cell activation and survival. It is also known as B7.1. The cDNA for B7-1 predicts a type I membrane protein, i.e., one synthesized with a signal peptide that is cleaved upon translocation across the endoplasmic membrane. The protein is predicted to contain 2 extracellular domains structurally similar to those of Ig, a hydrophobic transmembrane region, and a short cytoplasmic domain[1].The CD80 and CD86 (601020) genes encode B7-1 and B7-2, respectively, which are structurally similar members of the immunoglobulin superfamily expressed on a variety of hematopoietic cell types.[2] stated that B7-1 and B7-2 provide a costimulatory signal to T cells by interacting with CD28 and CTLA4. The standard product used in this kit is recombinant B7-1,D37K245,which is composed of two single chains acids with the dipolymer.
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 Typical Testing Data/Standard Curve (for reference only) of CD80 elisa kit    Mouse B7-1/CD80 PicoKine ELISA Kit standard curve
CD80 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS175825. Click to request current manual
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NCBI/Uniprot data below describe general gene information for CD80. It may not necessarily be applicable to this product.
 NCBI GI #    461607
 NCBI GeneID    12519
 NCBI Accession #    Q00609.1 [Other Products]
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 UniProt Primary Accession #    Q00609 [Other Products]
 UniProt Secondary Accession #    Q61332 [Other Products]
 UniProt Related Accession #    Q00609 [Other Products]
 Molecular Weight    24,051 Da
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 NCBI Official Full Name    T-lymphocyte activation antigen CD80
 NCBI Official Synonym Full Names    CD80 antigen
 NCBI Official Symbol    Cd80 [Similar Products]
 NCBI Official Synonym Symbols   
B71; Ly53; TSA1; Cd28l; Ly-53; MIC17
[Similar Products]
 NCBI Protein Information    T-lymphocyte activation antigen CD80; B7 protein; activation B7-1 antigen
 UniProt Protein Name    T-lymphocyte activation antigen CD80
 UniProt Synonym Protein Names   
Activation B7-1 antigen; B7; CD_antigen: CD80
 Protein Family    T-lymphocyte activation antigen
 UniProt Gene Name    Cd80 [Similar Products]
 UniProt Synonym Gene Names    B7; B7 [Similar Products]
 UniProt Entry Name    CD80_MOUSE
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 UniProt Comments for CD80    CD80: Involved in the costimulatory signal essential for T- lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor.

Protein type: Membrane protein, integral; Immunoglobulin superfamily

Cellular Component: cell surface; membrane; integral to membrane; external side of plasma membrane

Molecular Function: protein binding

Biological Process: positive regulation of peptidyl-tyrosine phosphorylation; T cell costimulation; positive regulation of alpha-beta T cell proliferation; positive regulation of T cell proliferation
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 Research Articles on CD80    1. The need for additional immune suppression in the intestine reflects commensal microbe-driven T-cell activation through the accessory costimulation molecules ICOSL and OX40L in B7 deprived mice.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with CD80 elisa kitDiseases associated with CD80 elisa kit
 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Autoimmune Thyroid Disease Pathway antibodies  Autoimmune Thyroid Disease Pathway Diagram
 Autoimmune Thyroid Disease Pathway antibodies  Autoimmune Thyroid Disease Pathway Diagram
 CD28 Co-stimulation Pathway antibodies  CD28 Co-stimulation Pathway Diagram
 CD28 Dependent PI3K/Akt Signaling Pathway antibodies  CD28 Dependent PI3K/Akt Signaling Pathway Diagram
 CD28 Dependent Vav1 Pathway antibodies  CD28 Dependent Vav1 Pathway Diagram
 CTLA4 Inhibitory Signaling Pathway antibodies  CTLA4 Inhibitory Signaling Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Disease Name  Pubmed Publications
 Necrosis Antibodies  >1099 publications with CD80 and Necrosis
 Inflammation Antibodies  >728 publications with CD80 and Inflammation
 Neoplasms, Experimental Antibodies  >376 publications with CD80 and Neoplasms, Experimental
 Nervous System Diseases Antibodies  >342 publications with CD80 and Nervous System Diseases
 Lung Diseases Antibodies  >301 publications with CD80 and Lung Diseases
 Liver Diseases Antibodies  >221 publications with CD80 and Liver Diseases
 Kidney Diseases Antibodies  >149 publications with CD80 and Kidney Diseases
 Lung Neoplasms Antibodies  >110 publications with CD80 and Lung Neoplasms
 Neoplasm Metastasis Antibodies  >81 publications with CD80 and Neoplasm Metastasis
 Breast Neoplasms Antibodies  >80 publications with CD80 and Breast Neoplasms
Organs/Tissues associated with CD80 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Bone Marrow Antibodies  >893 publications with CD80 and Bone Marrow
 Skin Antibodies  >385 publications with CD80 and Skin
 Thymus Antibodies  >177 publications with CD80 and Thymus
 Brain Antibodies  >163 publications with CD80 and Brain
 Muscle Antibodies  >82 publications with CD80 and Muscle
 Connective Tissue Antibodies  >33 publications with CD80 and Connective Tissue
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