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BDNF elisa kit :: Mouse BDNF ELISA Kit

Scan QR to view Datasheet Catalog #    MBS175800
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
96-Strip-Wells  /  $325 +1 FREE 8GB USB
 
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 Product Name   

BDNF, ELISA Kit

★Popular Item★
 Also Known As   

Mouse BDNF PicoKine ELISA Kit

 Product Synonym Names    Brain-derived neurotrophic factor; brain-derived neurotrophic factor; Abrineurin; ANON2; Brain derived neurotrophic factor; BULN2; MGC34632; Neurotrophin
 Product Gene Name   

BDNF elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 3D Structure    ModBase 3D Structure for P21237
 Species Reactivity    Mouse
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 Specificity    Natural and recombinant Mouse BDNF
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 Samples    Cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA, citrate)
 Assay Type    Quantitative Sandwich
 Detection Range    31.2pg/ml-2000pg/ml
 Sensitivity    <2pg/ml
 Intra-assay Precision    Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
 Inter-assay Precision    Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
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 Preparation and Storage    Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of BDNF elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for BDNF purchase    MBS175800 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the BDNF, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 31.2pg/ml-2000pg/ml in biological research samples containing BDNF, with an estimated sensitivity of <2pg/ml. The ELISA analytical biochemical technique of the MBS175800 kit is based on BDNF antibody-BDNF antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect BDNF antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, BDNF. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA, citrate). Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for BDNF elisa kit

   Principle of the Assay: Mouse BDNF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for BDNF has been precoated onto 96-well plates. Standards(Expression system for standard: sf21; Immunogen sequence: H129-R247) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for BDNF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin- Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse BDNF amount of sample captured in plate.

Background/Introduction: Brain-derived neurotrophic factor(BDNF) is a prosurvival factor induced by cortical neurons that is necessary for survival of striatal neurons in the brain. It is a secreted protein with the molecular weight of 27.8kDa, consisting of 247 amino acids. It is known to promote neuronal survival and differentiation. BDNF shares substantial amino acid sequence identity with nerve growth factor(NGF). BDNF and neurotrophin-3(NT-3) are two recently cloned neurotrophic factors that are homologous to NGF. mRNA products of the BDNF and NT-3 genes are detected in the adult human brain, suggesting that these proteins are involved in the maintenance of the adult nervous system. BDNF and other neurotrophins are critically involved in long-term potentiation(LTP). BDNF-mediated LTP is induced postsynaptically. BDNF has trophic effects on serotonergic(5-HT) neurons in the central nervous system. BDNF has an essential maintenance function in the regulation of anxiety-related behavior and in food intake through central mediators in both the basal and fasted state. It plays a role in treating breathing disorders such as respiratory insufficiency after spinal injury. The mature form of BDNF is identical in all mammals examined, and the gene encoding human BDNF to chromosome 11, band p13.
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 Typical Testing Data/Standard Curve (for reference only) of BDNF elisa kit    Mouse BDNF PicoKine ELISA Kit standard curve
BDNF elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS175800. Click to request current manual
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NCBI/Uniprot data below describe general gene information for BDNF. It may not necessarily be applicable to this product.
 NCBI GI #    114901
 NCBI GeneID    12064
 NCBI Accession #    P21237.1 [Other Products]
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 UniProt Primary Accession #    P21237 [Other Products]
 UniProt Related Accession #    P21237 [Other Products]
 Molecular Weight    28,123 Da
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 NCBI Official Full Name    Brain-derived neurotrophic factor
 NCBI Official Synonym Full Names    brain derived neurotrophic factor
 NCBI Official Symbol    Bdnf [Similar Products]
 NCBI Protein Information    brain-derived neurotrophic factor; anorexia BDNF
 UniProt Protein Name    Brain-derived neurotrophic factor
 Protein Family    BDNF/NT-3 growth factors receptor
 UniProt Gene Name    Bdnf [Similar Products]
 UniProt Synonym Gene Names    BDNF [Similar Products]
 UniProt Entry Name    BDNF_MOUSE
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 NCBI Summary for BDNF    The protein encoded by this gene is a member of the nerve growth factor family. It is involved in the growth, differentiation and survival of specific types of developing neurons both in the central nervous system (CNS) and the peripheral nervous system. It is also involved in regulating synaptic plasticity in the CNS. Expression of a similar gene in human is reduced in both Alzheimer's and Huntington disease patients. Multiple transcript variants encoding distinct isoforms have been described for this gene. [provided by RefSeq, Sep 2015]
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 UniProt Comments for BDNF    BDNF: During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. Defects in BDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. CCHS is frequently complicated with neurocristopathies such as Hirschsprung disease that occurs in about 16% of CCHS cases. Belongs to the NGF-beta family. 5 isoforms of the human protein are produced by alternative promoter.

Protein type: Secreted; Cytokine; Cell development/differentiation; Secreted, signal peptide

Cellular Component: extracellular space; cell; cytoplasmic membrane-bound vesicle; dendrite; extracellular region; terminal button; perikaryon; synaptic vesicle; mitochondrial crista; cell soma; perinuclear region of cytoplasm; axon; cytoplasm

Molecular Function: protein binding; growth factor activity; neurotrophin TRKB receptor binding; receptor binding

Biological Process: regulation of long-term neuronal synaptic plasticity; fear response; axon guidance; mechanoreceptor differentiation; behavioral fear response; mitochondrial electron transport, NADH to ubiquinone; axon extension; regulation of synaptic plasticity; positive regulation of long-term neuronal synaptic plasticity; regulation of inhibitory postsynaptic membrane potential; learning and/or memory; negative regulation of neuroblast proliferation; ureteric bud development; regulation of neuron apoptosis; dendrite development; regulation of retinal cell programmed cell death; feeding behavior; regulation of metabolic process; negative regulation of neuron apoptosis; negative regulation of synaptic transmission, GABAergic; inner ear development; response to drug; nervous system development; neuron recognition; regulation of axon extension; regulation of short-term neuronal synaptic plasticity; learning; negative regulation of striated muscle development; positive regulation of peptidyl-serine phosphorylation; positive regulation of synaptogenesis; axon target recognition; glutamate secretion; positive regulation of transcription factor activity; nerve development; regulation of excitatory postsynaptic membrane potential; positive regulation of neuron differentiation; gamma-aminobutyric acid signaling pathway; transmembrane receptor protein tyrosine kinase signaling pathway; negative regulation of apoptosis
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 Research Articles on BDNF    1. Significant reduction in the BDNF (P < 0.003) and c-Fos (P < 0.01) protein expression was observed in the HHL-6 treated group.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with BDNF elisa kitDiseases associated with BDNF elisa kit
 Products by Pathway  Pathway Diagram
 Alcoholism Pathway antibodies  Alcoholism Pathway Diagram
 Alcoholism Pathway antibodies  Alcoholism Pathway Diagram
 Cocaine Addiction Pathway antibodies  Cocaine Addiction Pathway Diagram
 Cocaine Addiction Pathway antibodies  Cocaine Addiction Pathway Diagram
 Huntington's Disease Pathway antibodies  Huntington's Disease Pathway Diagram
 Huntington's Disease Pathway antibodies  Huntington's Disease Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 Neurotrophin Signaling Pathway antibodies  Neurotrophin Signaling Pathway Diagram
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >3623 publications with BDNF and Nervous System Diseases
 Brain Injuries Antibodies  >841 publications with BDNF and Brain Injuries
 Depressive Disorder Antibodies  >676 publications with BDNF and Depressive Disorder
 Schizophrenia Antibodies  >568 publications with BDNF and Schizophrenia
 Brain Ischemia Antibodies  >554 publications with BDNF and Brain Ischemia
 Body Weight Antibodies  >551 publications with BDNF and Body Weight
 Cognition Disorders Antibodies  >545 publications with BDNF and Cognition Disorders
 Nerve Degeneration Antibodies  >543 publications with BDNF and Nerve Degeneration
 Inflammation Antibodies  >542 publications with BDNF and Inflammation
 Memory Disorders Antibodies  >521 publications with BDNF and Memory Disorders
Organs/Tissues associated with BDNF elisa kit
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >13981 publications with BDNF and Brain
 Eye Antibodies  >636 publications with BDNF and Eye
 Embryonic Tissue Antibodies  >558 publications with BDNF and Embryonic Tissue
 Dorsal Root Ganglion Antibodies  >442 publications with BDNF and Dorsal Root Ganglion
 Bone Antibodies  >379 publications with BDNF and Bone
 Lung Antibodies  >117 publications with BDNF and Lung
 Connective Tissue Antibodies  >111 publications with BDNF and Connective Tissue
 Tongue Antibodies  >63 publications with BDNF and Tongue
 Pituitary Gland Antibodies  >57 publications with BDNF and Pituitary Gland
 Testis Antibodies  >17 publications with BDNF and Testis
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