Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow ELISA Kit rightarrow BMP4 rightarrow Mouse BMP4  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • fMet-Leu-Phe receptor (Fpr1) Recombinant Protein
 • BECN1 ELISA Kit
 • angiostatin (ANG) ELISA Kit
 • CD31 - PECAM-1 (PECAM1) Antibody
 • DAB chromogenic kit (blue) IHC Related Reagent
 • Myostatin ELISA Kit
 • TERT Antibody
 • CD80 Antibody
 • CEL-BSA Control Native Protein
 • IL-1 beta (IL1B) Antibody
 • CDH2 Antibody
 • CD86 molecule ELISA Kit
 • Dna Damage Inducible Transcript 3 (DDIT3) ELISA Kit
 • proteasome (prosome, macropain) 26S subunit, non-ATPase, 4 (... ELISA Kit
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

BMP4 elisa kit :: Mouse BMP4 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS8248420
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
96 Tests  /  $470 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

BMP4, ELISA Kit

★Popular Item★
 Also Known As   

Mouse BMP4 ELISA Kit

 Product Synonym Names    BMP2B; DVR4; Bone morphogenetic protein 4; BMP-4; Bone morphogenetic protein 2B; BMP-2B
 Product Gene Name   

BMP4 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 3D Structure    ModBase 3D Structure for P21275
 Species Reactivity    Mouse
Section Bar
 Specificity    The Mouse BMP4 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Mouse BMP4 proteins within the range of 31.2 pg/ml - 2000 pg/ml.
Section Bar
 Samples    Bone Tissue, Cell Culture Supernates, Serum
 Sensitivity    <10 pg/ml
Section Bar
 Preparation and Storage    Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of BMP4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for BMP4 purchase    MBS8248420 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the BMP4, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing BMP4, with an estimated sensitivity of <10 pg/ml. The ELISA analytical biochemical technique of the MBS8248420 kit is based on BMP4 antibody-BMP4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect BMP4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, BMP4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Bone Tissue, Cell Culture Supernates, Serum. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Table BarTOPTable Bar
 

Product Description specifically for BMP4 elisa kit

   Principle of the Assay: The Mouse BMP4 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse BMP4 in Bone Tissue, Cell Culture Supernates, Serum. This assay employs an antibody specific for Mouse BMP4 coated on a 96-well plate. Standards and samples are pipetted into the wells and BMP4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse BMP4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of BMP4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background: Bone morphogenetic protein 4 is a protein that in humans is encoded by the BMP4 gene which is located to 14q22-q23. The protein encoded by this gene is a member of the bone morphogenetic protein family which is part of the transforming growth factor-beta superfamily. BMP4 is a polypeptide belonging to the TGF-beta superfamily of proteins. It, like other bone morphogenetic proteins, is involved in bone and cartilage development, specifically tooth and limb development and fracture repair. It has been shown to be involved in muscle development, bone mineralization, and uteric bud development. BMP4 has also been implicated in Fibrodysplasia Ossificans Progressiva in which it is underexpressed. In human embryonic development, BMP4 is a critical signaling molecule required for the early differentiation of the embryo and establishing of a dorsal-ventral axis. BMP4 is secreted from the dorsal portion of the notochord, and it acts in concert with sonic hedgehog (released from the ventral portion of the notochord) to establish a dorsal-ventral axis for the differentiation of later structures. BMP4 stimulates differentiation of overlying ectodermal tissue. Inhibition of the BMP4 signal (by chordin, noggin, or follistatin) causes the ectoderm to differentiate into the neural plate. The standard product used in this kit is recombinant BMP-4 with the molecular mass of 26KDa.
Section Bar
 Typical Testing Data/Standard Curve (for reference only) of BMP4 elisa kit    BMP4 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Table BarTOPTable Bar
Sample Manual Insert of MBS8248420. Click to request current manual
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for BMP4. It may not necessarily be applicable to this product.
 NCBI GI #    939320322
 NCBI GeneID    12159
 NCBI Accession #    NP_001303289.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001316360.1 [Other Products]
Section Bar
 UniProt Primary Accession #    P21275 [Other Products]
 UniProt Related Accession #    P21275 [Other Products]
 Molecular Weight    46,497 Da
Table BarTOPTable Bar
 NCBI Official Full Name    bone morphogenetic protein 4 preproprotein
 NCBI Official Synonym Full Names    bone morphogenetic protein 4
 NCBI Official Symbol    Bmp4 [Similar Products]
 NCBI Official Synonym Symbols   
Bmp-4; Bmp2b; Bmp2b1; Bmp2b-1
[Similar Products]
 NCBI Protein Information    bone morphogenetic protein 4
 UniProt Protein Name    Bone morphogenetic protein 4
 UniProt Synonym Protein Names   
Bone morphogenetic protein 2B; BMP-2B
 Protein Family    Bone morphogenetic protein
 UniProt Gene Name    Bmp4 [Similar Products]
 UniProt Synonym Gene Names    Bmp-4; Dvr-4; BMP-4; BMP-2B [Similar Products]
 UniProt Entry Name    BMP4_MOUSE
Section Bar
 NCBI Summary for BMP4    This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein regulates heart development and adipogenesis. Homozygous knockout mice die in utero, while a conditional knockout mouse exhibits defects in heart development. Transgenic mice overexpressing this gene in a neuron-specific manner exhibit a phenotype resembling the rare hereditary connective tissue disease, fibrodysplasia ossificans progressiva. [provided by RefSeq, Jul 2016]
Section Bar
 UniProt Comments for BMP4    BMP4: Induces cartilage and bone formation. Also act in mesoderm induction, tooth development, limb formation and fracture repair. Acts in concert with PTHLH/PTHRP to stimulate ductal outgrowth during embryonic mammary development and to inhibit hair follicle induction. Homodimer; disulfide-linked. Interacts with GREM2. Part of a complex consisting of TWSG1 and CHRD. Interacts with the serine proteases, HTRA1 and HTRA3; the interaction with either inhibits BMP4-mediated signaling. The HTRA protease activity is required for this inhibition. Interacts with SOSTDC1. Expressed in the lung and lower levels seen in the kidney. Present also in normal and neoplastic prostate tissues, and prostate cancer cell lines. Belongs to the TGF-beta family.

Protein type: Secreted; Secreted, signal peptide

Cellular Component: cytoplasm; extracellular region; extracellular space; vesicle

Molecular Function: chemoattractant activity; cytokine activity; growth factor activity; heparin binding; protein binding; protein homodimerization activity; transforming growth factor beta receptor binding

Biological Process: activation of MAPKK activity; alveolus development; anatomical structure formation; angiogenesis; anterior/posterior axis specification; blood vessel development; blood vessel endothelial cell proliferation during sprouting angiogenesis; BMP signaling pathway; branching morphogenesis of a tube; camera-type eye development; camera-type eye morphogenesis; cardiac muscle cell differentiation; cell fate commitment; chondrocyte differentiation; cloacal septation; common-partner SMAD protein phosphorylation; dorsoventral neural tube patterning; embryonic cranial skeleton morphogenesis; embryonic digit morphogenesis; embryonic hindlimb morphogenesis; embryonic limb morphogenesis; embryonic morphogenesis; embryonic skeletal development; embryonic skeletal morphogenesis; endochondral ossification; endoderm development; erythrocyte differentiation; eye development; forebrain development; germ cell development; heart development; hemopoietic progenitor cell differentiation; induction of an organ; inner ear receptor cell differentiation; intermediate mesodermal cell differentiation; kidney development; lung development; lymphoid progenitor cell differentiation; macrophage differentiation; mesoderm formation; mesodermal cell differentiation; mesodermal cell fate determination; metanephros development; monocyte differentiation; negative regulation of apoptosis; negative regulation of cell cycle; negative regulation of cell proliferation; negative regulation of chondrocyte differentiation; negative regulation of epithelial cell proliferation; negative regulation of immature T cell proliferation in the thymus; negative regulation of MAP kinase activity; negative regulation of mitosis; negative regulation of myoblast differentiation; negative regulation of oligodendrocyte differentiation; negative regulation of phosphorylation; negative regulation of striated muscle development; negative regulation of T cell differentiation in the thymus; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; neural tube closure; neuron fate commitment; odontogenesis; odontogenesis of dentine-containing teeth; osteoblast differentiation; ovarian follicle development; pituitary gland development; positive regulation of apoptosis; positive regulation of BMP signaling pathway; positive regulation of bone mineralization; positive regulation of cardiac muscle fiber development; positive regulation of cell differentiation; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of collagen biosynthetic process; positive regulation of endothelial cell differentiation; positive regulation of endothelial cell proliferation; positive regulation of epidermal cell differentiation; positive regulation of epithelial cell proliferation; positive regulation of neuron differentiation; positive regulation of ossification; positive regulation of osteoblast differentiation; positive regulation of protein amino acid phosphorylation; positive regulation of protein binding; positive regulation of smooth muscle cell proliferation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; positive regulation of vascular endothelial growth factor receptor signaling pathway; post-embryonic development; regulation of cell differentiation; regulation of cell fate commitment; regulation of endothelial cell proliferation; regulation of gene expression; regulation of odontogenesis of dentine-containing teeth; regulation of protein import into nucleus; regulation of smooth muscle cell differentiation; regulation of smooth muscle cell proliferation; renal system process; skeletal development; smooth muscle cell differentiation; smooth muscle development; specification of organ position; steroid hormone mediated signaling; telencephalon development; telencephalon regionalization; tissue development; ureteric bud branching; ureteric bud development; vasculature development
Table BarTOPTable Bar
 Research Articles on BMP4    1. the present study suggested that miRNA378 is critical for the promotion of myoblast differentiation by targeting BMP4.
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Products associated with BMP4 elisa kitPathways associated with BMP4 elisa kit
 Reference Product  PubMed Publications
 Bmp7 elisa kit  >307 publications with BMP4 and Bmp7
 Shh elisa kit  >270 publications with BMP4 and Shh
 Bmpr2 elisa kit  >175 publications with BMP4 and Bmpr2
 Msx1 elisa kit  >166 publications with BMP4 and Msx1
 Bmpr1a elisa kit  >92 publications with BMP4 and Bmpr1a
 Bmpr1b elisa kit  >49 publications with BMP4 and Bmpr1b
 Smad2 elisa kit  >39 publications with BMP4 and Smad2
 Ptch1 elisa kit  >27 publications with BMP4 and Ptch1
 Nog elisa kit  >24 publications with BMP4 and Nog
 Chrd elisa kit  >6 publications with BMP4 and Chrd
 Products by Pathway  Pathway Diagram
 Adipogenesis Genes Pathway antibodies  Adipogenesis Genes Pathway Diagram
 BMP Signaling Pathway In Eyelid Development antibodies  BMP Signaling Pathway In Eyelid Development Diagram
 BMP Signaling Pathway antibodies  BMP Signaling Pathway Diagram
 BMP Signaling Pathway antibodies  BMP Signaling Pathway Diagram
 Basal Cell Carcinoma Pathway antibodies  Basal Cell Carcinoma Pathway Diagram
 Basal Cell Carcinoma Pathway antibodies  Basal Cell Carcinoma Pathway Diagram
 ESC Pluripotency Pathways antibodies  ESC Pluripotency Pathways Diagram
 Elastic Fibre Formation Pathway antibodies  Elastic Fibre Formation Pathway Diagram
 Extracellular Matrix Organization Pathway antibodies  Extracellular Matrix Organization Pathway Diagram
 Heart Development Pathway antibodies  Heart Development Pathway Diagram
Diseases associated with BMP4 elisa kitOrgans/Tissues associated with BMP4 elisa kit
 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >135 publications with BMP4 and Cardiovascular Diseases
 Disease Models, Animal Antibodies  >130 publications with BMP4 and Disease Models, Animal
 Nervous System Diseases Antibodies  >121 publications with BMP4 and Nervous System Diseases
 Heart Diseases Antibodies  >80 publications with BMP4 and Heart Diseases
 Hypertension Antibodies  >77 publications with BMP4 and Hypertension
 Inflammation Antibodies  >75 publications with BMP4 and Inflammation
 Brain Diseases Antibodies  >70 publications with BMP4 and Brain Diseases
 Lung Diseases Antibodies  >64 publications with BMP4 and Lung Diseases
 Necrosis Antibodies  >53 publications with BMP4 and Necrosis
 Fibrosis Antibodies  >43 publications with BMP4 and Fibrosis
 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >3019 publications with BMP4 and Bone
 Embryonic Tissue Antibodies  >745 publications with BMP4 and Embryonic Tissue
 Eye Antibodies  >184 publications with BMP4 and Eye
 Bone Marrow Antibodies  >168 publications with BMP4 and Bone Marrow
 Inner Ear Antibodies  >75 publications with BMP4 and Inner Ear
 Intestine Antibodies  >55 publications with BMP4 and Intestine
 Prostate Antibodies  >39 publications with BMP4 and Prostate
 Bladder Antibodies  >21 publications with BMP4 and Bladder
 Extraembryonic Tissue Antibodies  >19 publications with BMP4 and Extraembryonic Tissue
 Salivary Gland Antibodies  >3 publications with BMP4 and Salivary Gland
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions