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DEFbeta3 elisa kit :: Mouse Beta-Defensins 3 (DEFbeta3) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2600382
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $275 +1 FREE 8GB USB
96-Strip-Wells  /  $435 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,755 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,150 +3 FREE 8GB USB
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 Product Name   

Beta-Defensins 3 (DEFbeta3), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Beta-Defensins 3 (DEFbeta3) ELISA Kit

 Product Synonym Names    Mouse Beta-Defensins 3 (DEFb3) ELISA Kit
 Product Gene Name   

DEFbeta3 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    606611
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 Species Reactivity    Mouse
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 Specificity    No cross-reaction with other factors.
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 Samples    Mouse serum, plasma or cell culture supernatant and organizations in the natural and recombinant DEFbeta3 concentration.
 Detection Range    2000 pg/ml-31.2 pg/ml
 Sensitivity    Up to 12 pg/ml.
 Assay Type    Sandwich
 Intra-assay Precision    <= 8%
 Inter-assay Precision    <= 12%
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of DEFbeta3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for DEFbeta3 purchase    MBS2600382 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Beta-Defensins 3 (DEFbeta3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 2000 pg/ml-31.2 pg/ml in biological research samples containing DEFbeta3, with an estimated sensitivity of Up to 12 pg/ml. The ELISA analytical biochemical technique of the MBS2600382 kit is based on DEFbeta3 antibody-DEFbeta3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect DEFbeta3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, DEFbeta3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Mouse serum, plasma or cell culture supernatant and organizations in the natural and recombinant DEFbeta3 concentration. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for DEFbeta3 elisa kit

   Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse DEFbeta3 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
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 Typical Testing Data/Standard Curve (for reference only) of DEFbeta3 elisa kit    DEFbeta3 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2600382. Click to request current manual
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NCBI/Uniprot data below describe general gene information for DEFbeta3. It may not necessarily be applicable to this product.
 NCBI GI #    8923890
 NCBI GeneID    55894
 NCBI Accession #    NP_061131.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_018661.4 [Other Products]
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 UniProt Secondary Accession #    Q8NFG6; Q9NPF6 [Other Products]
 UniProt Related Accession #    P81534 [Other Products]
 Molecular Weight    7,697 Da
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 NCBI Official Full Name    beta-defensin 103
 NCBI Official Synonym Full Names    defensin, beta 103B
 NCBI Official Symbol    DEFB103B [Similar Products]
 NCBI Official Synonym Symbols   
BD-3; HBD3; HBP3; DEFB3; HBD-3; HBP-3; DEFB-3; DEFB103
[Similar Products]
 NCBI Protein Information    beta-defensin 103; beta-defensin 3; defensin, beta 103; defensin-like protein
 UniProt Protein Name    Beta-defensin 103
 UniProt Synonym Protein Names   
Beta-defensin 3; BD-3; DEFB-3; HBD3; hBD-3; Defensin, beta 103; Defensin-like protein
 UniProt Gene Name    DEFB103A [Similar Products]
 UniProt Synonym Gene Names    BD3; DEFB103; DEFB3
DEFB103B; BD-3; DEFB-3; HBD3; hBD-3
[Similar Products]
 UniProt Entry Name    D103A_HUMAN
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 NCBI Summary for DEFbeta3    Defensins form a family of microbicidal and cytotoxic peptides made by neutrophils. Members of the defensin family are highly similar in protein sequence. This gene encodes defensin, beta 103B, which has broad spectrum antimicrobial activity and may play an important role in innate epithelial defense. [provided by RefSeq, Jul 2008]
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 UniProt Comments for DEFbeta3    defensin, beta 3: Exhibits antimicrobial activity against Gram-positive bacteria S.aureus and S.pyogenes, Gram-negative bacteria P.aeruginosa and E.coli and the yeast C.albicans. Kills multiresistant S.aureus and vancomycin-resistant E.faecium. No significant hemolytic activity was observed. Belongs to the beta-defensin family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 8p23.1

Cellular Component: Golgi lumen; extracellular region

Biological Process: defense response to bacterium; innate immune response; positive regulation of biosynthetic process of antibacterial peptides active against Gram-positive bacteria
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 Research Articles on DEFbeta3    1. Data show that Lactobacillus crispatus promotes epithelial cell defense against Candida albicans infection through the involvement of TLR2/4, IL-8 and human beta-defensin 2 and 3, suggesting this Lactobacillus as an anti-infective agent against C. albicans.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with DEFbeta3 elisa kit
 Products by Pathway  Pathway Diagram
 Beta Defensins Pathway antibodies  Beta Defensins Pathway Diagram
 Defensins Pathway antibodies  Defensins Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Innate Immune System Pathway antibodies  Innate Immune System Pathway Diagram
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