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Defb3 elisa kit :: Mouse Beta-defensin 3 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS289613
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $380 +1 FREE 8GB USB
96-Strip-Wells  /  $500 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,170 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,840 +4 FREE 8GB USB
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 Product Name   

Beta-defensin 3 (Defb3), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Beta-defensin 3 ELISA Kit

 Product Synonym Names    Beta-defensin 3; BD-3; mBD-3; Defensin, beta 3; Defb3; Bd3
 Product Gene Name   

Defb3 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS289613 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for Q9WTL0
 Species Reactivity    Mouse
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 Specificity    Recombinant and natural Mouse BD-3
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 Assay Type    Sandwich
 Detection Range    0.156-10 ng/mL
 Sensitivity    0.09 ng/mL
 Intra-Assay CV    <=5.7%
 Inter-Assay CV    <=9.1%
 Recovery    100%
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 Preparation and Storage    For long term storage, please store the entire kit at -20 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of Defb3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for Defb3 purchase    MBS289613 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Beta-defensin 3 (Defb3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.156-10 ng/mL in biological research samples containing Defb3, with an estimated sensitivity of 0.09 ng/mL. The ELISA analytical biochemical technique of the MBS289613 kit is based on Defb3 antibody-Defb3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Defb3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Defb3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for Defb3 elisa kit

   Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of Defb3 elisa kit    Defb3 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS289613. Click to request current manual
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NCBI/Uniprot data below describe general gene information for Defb3. It may not necessarily be applicable to this product.
 NCBI GI #    7304997
 NCBI GeneID    27358
 NCBI Accession #    NP_038784.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_013756.2 [Other Products]
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 UniProt Primary Accession #    Q9WTL0 [Other Products]
 UniProt Secondary Accession #    Q29R78 [Other Products]
 UniProt Related Accession #    Q9WTL0 [Other Products]
 Molecular Weight    7,126 Da
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 NCBI Official Full Name    beta-defensin 3
 NCBI Official Synonym Full Names    defensin beta 3
 NCBI Official Symbol    Defb3 [Similar Products]
 NCBI Official Synonym Symbols   
[Similar Products]
 NCBI Protein Information    beta-defensin 3
 UniProt Protein Name    Beta-defensin 3
 UniProt Synonym Protein Names   
Defensin, beta 3
 Protein Family    Beta-defensin
 UniProt Gene Name    Defb3 [Similar Products]
 UniProt Synonym Gene Names    Bd3; BD-3; mBD-3 [Similar Products]
 UniProt Entry Name    DEFB3_MOUSE
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 UniProt Comments for Defb3    Antimicrobial activity against Gram-negative bacteria E.coli and P.aeruginosa.
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 Research Articles on Defb3    1. Stress decreases mouse beta-defensin-3 expression in the esophagus of mice with diabetes.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with Defb3 elisa kitDiseases associated with Defb3 elisa kit
 Reference Product  PubMed Publications
 Defb1 elisa kit  >2 publications with Defb3 and Defb1
 Defb14 elisa kit  >1 publications with Defb3 and Defb14
 S100a8 elisa kit  >1 publications with Defb3 and S100a8
 Defb2 elisa kit  >1 publications with Defb3 and Defb2
 Disease Name  Pubmed Publications
 Gastrointestinal Diseases Antibodies  >1 publications with Defb3 and Gastrointestinal Diseases
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