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BOLA3 elisa kit :: Mouse BolA-like protein 3 (BOLA3) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS7207186
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $440 +1 FREE 8GB USB
96-Strip-Wells  /  $640 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
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 Product Name   

BolA-like protein 3 (BOLA3), ELISA Kit

★Popular Item★
 Also Known As   

Mouse BolA-like protein 3 (BOLA3) ELISA Kit

 Product Gene Name   

BOLA3 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    gene 614299
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 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of BOLA3. No significant cross-reactivity or interference between BOLA3 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between BOLA3 and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Sensitivity    1.0 pg/mL.
 Intended Uses    This BOLA3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse BOLA3. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of BOLA3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for BOLA3 purchase    MBS7207186 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the BolA-like protein 3 (BOLA3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing BOLA3, with an estimated sensitivity of 1.0 pg/mL. The ELISA analytical biochemical technique of the MBS7207186 kit is based on BOLA3 antibody-BOLA3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect BOLA3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, BOLA3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for BOLA3 elisa kit

   Principle of the assay: BOLA3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-BOLA3 antibody and an BOLA3-HRP conjugate. The assay sample and buffer are incubated together with BOLA3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BOLA3 concentration since BOLA3 from samples and BOLA3-HRP conjugate compete for the anti-BOLA3 antibody binding site. Since the number of sites is limited, as more sites are occupied by BOLA3 from the sample, fewer sites are left to bind BOLA3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BOLA3 concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for BOLA3 elisa kit    Cell Biology
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 Typical Testing Data/Standard Curve (for reference only) of BOLA3 elisa kit    BOLA3 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS7207186. Click to request current manual
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NCBI/Uniprot data below describe general gene information for BOLA3. It may not necessarily be applicable to this product.
 NCBI GI #    78486586
 NCBI GeneID    388962
 NCBI Accession #    NP_997717.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_212552.2 [Other Products]
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 UniProt Secondary Accession #    G3XAB0 [Other Products]
 UniProt Related Accession #    Q53S33 [Other Products]
 Molecular Weight    11,569 Da
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 NCBI Official Full Name    bolA-like protein 3 isoform 1
 NCBI Official Synonym Full Names    bolA family member 3
 NCBI Official Symbol    BOLA3 [Similar Products]
 NCBI Official Synonym Symbols   
[Similar Products]
 NCBI Protein Information    bolA-like protein 3; bolA homolog 3
 UniProt Protein Name    BolA-like protein 3
 Protein Family    BolA-like protein
 UniProt Gene Name    BOLA3 [Similar Products]
 UniProt Entry Name    BOLA3_HUMAN
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 NCBI Summary for BOLA3    This gene encodes a protein that plays an essential role in the production of iron-sulfur (Fe-S) clusters for the normal maturation of lipoate-containing 2-oxoacid dehydrogenases, and for the assembly of the mitochondrial respiratory chain complexes. Mutation in this gene has been associated with multiple mitochondrial dysfunctions syndrome-2. Two alternatively spliced transcript variants encoding different isoforms with distinct subcellular localization have been reported for this gene (PMID:21944046). [provided by RefSeq, Dec 2011]
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 UniProt Comments for BOLA3    BOLA3: Defects in BOLA3 are the cause of multiple mitochondrial dysfunctions syndrome type 2 (MMDS2). A severe disorder of systemic energy metabolism, resulting in weakness, respiratory failure, lack of neurologic development, lactic acidosis, hyperglycinemia and early death. Some patients show failure to thrive, pulmonary hypertension, hypotonia and irritability. Biochemical features include severe combined deficiency of the 2-oxoacid dehydrogenases, defective lipoic acid synthesis and reduction in activity of mitochondrial respiratory chain complexes. Belongs to the bolA/yrbA family.

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 2p13.1

Cellular Component: extracellular region

Disease: Multiple Mitochondrial Dysfunctions Syndrome 2
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 Research Articles on BOLA3    1. Clinical features of BOLA3-associated variant nonketotic hyperglycemia include severe neurodegeneration after a period of normal development.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with BOLA3 elisa kitDiseases associated with BOLA3 elisa kit
 Reference Product  PubMed Publications
 GLRX5 elisa kit  >2 publications with BOLA3 and GLRX5
 BOLA1 elisa kit  >2 publications with BOLA3 and BOLA1
 BOLA2 elisa kit  >2 publications with BOLA3 and BOLA2
 Disease Name  Pubmed Publications
 Atrophy Antibodies  >1 publications with BOLA3 and Atrophy
Organs/Tissues associated with BOLA3 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >1 publications with BOLA3 and Muscle
 Blood Antibodies  >1 publications with BOLA3 and Blood
 Brain Antibodies  >1 publications with BOLA3 and Brain
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