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BALP elisa kit :: Mouse Bone Alkaline Phosphatase ELISA Kit

Scan QR to view Datasheet Catalog #    MBS731438
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $440 +1 FREE 8GB USB
96-Strip-Wells  /  $640 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
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 Product Name   

Bone Alkaline Phosphatase (BALP), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Bone Alkaline Phosphatase ELISA Kit

 Product Gene Name   

BALP elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of BALP. No significant cross-reactivity or interference between BALP and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between BALP and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Detection Range    0.5-10ng/mL
 Sensitivity    0.1ng/mL
 Intended Uses    This BALP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse BALP. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of BALP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for BALP purchase    MBS731438 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Bone Alkaline Phosphatase (BALP) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.5-10ng/mL in biological research samples containing BALP, with an estimated sensitivity of 0.1ng/mL. The ELISA analytical biochemical technique of the MBS731438 kit is based on BALP antibody-BALP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect BALP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, BALP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%). Bones are created, bones are destroyed. It's an ongoing struggle and as we age, the balance tips to loss. Research into the differentiation of bone cells and required bone morphogenetic protein require detection of bone alkaline phosphatase. Mouse bone alkaline phosphatase BALP is a biomarker for the differentiation in cell culture models of human osteoporosis. For the greatest sensitivity and dynamic range, we recommend our BALP ELISA kit. With ng ml sample precision, prebound assay plate, sample diluent, secondary antibodies, standard protein and rapid performance, our ELISA kit from CUSABio will meet your needs. We have samples concentration tested twenty times to validate assay precision. These ELISA kits are ideal for your small molecule screening assays and meet CLIA kits standards for molecular biology reagents. Full application details and potential target details come with kit product. We also offer BALP ELISA kit human and ELISA kit rat. Order today!
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Related Product Information for BALP elisa kit

   Principle of the assay: BALP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-BALP antibody and an BALP-HRP conjugate. The assay sample and buffer are incubated together with BALP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BALP concentration since BALP from samples and BALP-HRP conjugate compete for the anti-BALP antibody binding site. Since the number of sites is limited, as more sites are occupied by BALP from the sample, fewer sites are left to bind BALP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BALP concentration in each sample is interpolated from this standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of BALP elisa kit    BALP elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS731438. Click to request current manual
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NCBI/Uniprot data below describe general gene information for BALP. It may not necessarily be applicable to this product.
 NCBI GI #    194133809
 NCBI Accession #    EDW55325.1 [Other Products]
 Molecular Weight    42,112 Da
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 NCBI Official Full Name    bap
 UniProt Protein Name    BAP
 UniProt Synonym Protein Names   
BAP; Bap
 UniProt Gene Name    bap [Similar Products]
 UniProt Synonym Gene Names    Dsecap; Dsec_GM10423; GM10423 [Similar Products]
 UniProt Entry Name    B2ZIB7_DROSE
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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