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ALP-B elisa kit :: Mouse Bone-specific Alkphase B (ALP-B) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS264117
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $275 +1 FREE 8GB USB
96-Strip-Wells  /  $435 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,755 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,150 +3 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Bone-specific Alkphase B (ALP-B), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Bone-specific Alkphase B (ALP-B) ELISA Kit

 Product Gene Name   

ALP-B elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    609212
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 Species Reactivity    Mouse
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 Specificity    No cross-reaction with other factors.
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 Samples    Mouse serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant ALP-B concentration
 Assay Type    Sandwich
 Detection Range    100 ng/ml-1.56 ng/ml
 Sensitivity    0.5ng/ml.
 Intra-assay Precision    <= 8%
 Inter-assay Precision    <= 12%
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of ALP-B elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for ALP-B purchase    MBS264117 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Bone-specific Alkphase B (ALP-B) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 100 ng/ml-1.56 ng/ml in biological research samples containing ALP-B, with an estimated sensitivity of 0.5ng/ml. The ELISA analytical biochemical technique of the MBS264117 kit is based on ALP-B antibody-ALP-B antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ALP-B antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ALP-B. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Mouse serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant ALP-B concentration. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for ALP-B elisa kit

   Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse ALP-B monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
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 Typical Testing Data/Standard Curve (for reference only) of ALP-B elisa kit    ALP-B elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS264117. Click to request current manual
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NCBI/Uniprot data below describe general gene information for ALP-B. It may not necessarily be applicable to this product.
 NCBI GI #    127798426
 NCBI GeneID    80150
 NCBI Accession #    AAH21295.3 [Other Products]
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 UniProt Secondary Accession #    Q567Q4; Q6P1P0; Q8NI34; Q9H6F7; B2R7Q0 [Other Products]
 UniProt Related Accession #    Q7L266 [Other Products]
 Molecular Weight    18,981 Da
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 NCBI Official Full Name    Asparaginase like 1
 NCBI Official Synonym Full Names    asparaginase like 1
 NCBI Official Symbol    ASRGL1 [Similar Products]
 NCBI Official Synonym Symbols   
ALP; ALP1; CRASH
[Similar Products]
 NCBI Protein Information    isoaspartyl peptidase/L-asparaginase; beta-aspartyl-peptidase; isoaspartyl dipeptidase; L-asparagine amidohydrolase; asparaginase-like 1 protein; asparaginase-like protein 1
 UniProt Protein Name    Isoaspartyl peptidase/L-asparaginase
 UniProt Synonym Protein Names   
Asparaginase-like protein 1; Beta-aspartyl-peptidase; Isoaspartyl dipeptidase; L-asparagine amidohydrolaseCleaved into the following 2 chains:Isoaspartyl peptidase/L-asparaginase alpha chain; Isoaspartyl peptidase/L-asparaginase beta chain
 UniProt Gene Name    ASRGL1 [Similar Products]
 UniProt Synonym Gene Names    ALP; CRASH [Similar Products]
 UniProt Entry Name    ASGL1_HUMAN
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 UniProt Comments for ALP-B    ASRGL1: Acts in asparagine catabolism. May be involved in astroglial production of L-aspartate, which can act as an excitatory neurotransmitter in some brain regions. Belongs to the Ntn-hydrolase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Other Amino Acids Metabolism - cyanoamino acid; Energy Metabolism - nitrogen; Amino Acid Metabolism - alanine, aspartate and glutamate; Hydrolase; EC 3.5.1.1; EC 3.4.19.5

Chromosomal Location of Human Ortholog: 11q12.3

Cellular Component: cytoplasm; nucleus

Molecular Function: asparaginase activity; N4-(beta-N-acetylglucosaminyl)-L-asparaginase activity; beta-aspartyl-peptidase activity

Biological Process: asparagine catabolic process via L-aspartate; proteolysis
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 Research Articles on ALP-B    1. Determined is the structure of the first mammalian plant-type asparaginase in both a precursor and fully activated form.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with ALP-B elisa kitDiseases associated with ALP-B elisa kit
 Products by Pathway  Pathway Diagram
 Asparagine Degradation I Pathway antibodies  Asparagine Degradation I Pathway Diagram
 Disease Name  Pubmed Publications
 Ovarian Neoplasms Antibodies  >2 publications with ALP-B and Ovarian Neoplasms
 Breast Neoplasms Antibodies  >2 publications with ALP-B and Breast Neoplasms
 Prostatic Neoplasms Antibodies  >1 publications with ALP-B and Prostatic Neoplasms
 Adenocarcinoma Antibodies  >1 publications with ALP-B and Adenocarcinoma
Organs/Tissues associated with ALP-B elisa kit
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >3 publications with ALP-B and Brain
 Testis Antibodies  >2 publications with ALP-B and Testis
 Esophagus Antibodies  >1 publications with ALP-B and Esophagus
 Heart Antibodies  >1 publications with ALP-B and Heart
 Muscle Antibodies  >1 publications with ALP-B and Muscle
 Blood Antibodies  >1 publications with ALP-B and Blood
 Liver Antibodies  >1 publications with ALP-B and Liver
 Prostate Antibodies  >1 publications with ALP-B and Prostate
 Ovary Antibodies  >1 publications with ALP-B and Ovary
 Kidney Antibodies  >1 publications with ALP-B and Kidney
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