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CXCL3 elisa kit :: Mouse C-X-C motif chemokine 3 (CXCL3) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS7238613
Typical Standard Curve/Testing Data
Unit / Price
48-Strip-Wells  /  $440 +1 FREE 8GB USB
96-Strip-Wells  /  $640 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

C-X-C motif chemokine 3 (CXCL3), ELISA Kit

★Popular Item★
 Also Known As   

Mouse C-X-C motif chemokine 3 (CXCL3) ELISA Kit

 Product Gene Name   

CXCL3 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    139111
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 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of CXCL3. No significant cross-reactivity or interference between CXCL3 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CXCL3 and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
 Assay Type    Quantitative Competitive
 Sensitivity    0.1 ng/mL.
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of CXCL3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for CXCL3 purchase    MBS7238613 is a ready-to-use microwell, strip plate Quantitative Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the C-X-C motif chemokine 3 (CXCL3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CXCL3, with an estimated sensitivity of 0.1 ng/mL. The ELISA analytical biochemical technique of the MBS7238613 kit is based on CXCL3 antibody-CXCL3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CXCL3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CXCL3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for CXCL3 elisa kit

   Intended Uses: This CXCL3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse CXCL3. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: CXCL3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CXCL3 antibody and an CXCL3-HRP conjugate. The assay sample and buffer are incubated together with CXCL3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CXCL3 concentration since CXCL3 from samples and CXCL3-HRP conjugate compete for the anti-CXCL3 antibody binding site. Since the number of sites is limited, as more sites are occupied by CXCL3 from the sample, fewer sites are left to bind CXCL3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CXCL3 concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for CXCL3 elisa kit    Immunology
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 Typical Standard Curve/Testing Data of CXCL3 elisa kit    CXCL3 elisa kit Typical Standard Curve/Testing Data image
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Sample Manual Insert of MBS7238613. Click to request current manual
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NCBI/Uniprot data below describe general gene information for CXCL3. It may not necessarily be applicable to this product.
 NCBI GI #    54144650
 NCBI GeneID    2921
 NCBI Accession #    NP_002081.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_002090.2 [Other Products]
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 UniProt Secondary Accession #    Q4W5H9 [Other Products]
 UniProt Related Accession #    P19876 [Other Products]
 Molecular Weight    11,342 Da
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 NCBI Official Full Name    C-X-C motif chemokine 3
 NCBI Official Synonym Full Names    chemokine (C-X-C motif) ligand 3
 NCBI Official Symbol    CXCL3 [Similar Products]
 NCBI Official Synonym Symbols   
GRO3; GROg; MIP2B; SCYB3; MIP-2b; CINC-2b
[Similar Products]
 NCBI Protein Information    C-X-C motif chemokine 3; GRO-gamma; MIP2-beta; MGSA gamma; GRO3 oncogene; GRO-gamma(1-73); growth-regulated protein gamma; macrophage inflammatory protein 2-beta; melanoma growth stimulatory activity gamma
 UniProt Protein Name    C-X-C motif chemokine 3
 UniProt Synonym Protein Names   
GRO-gamma(1-73); Growth-regulated protein gamma; GRO-gamma; Macrophage inflammatory protein 2-beta; MIP2-beta
 Protein Family    C-X-C motif chemokine
 UniProt Gene Name    CXCL3 [Similar Products]
 UniProt Synonym Gene Names    GRO3; GROG; SCYB3; GRO-gamma; MIP2-beta [Similar Products]
 UniProt Entry Name    CXCL3_HUMAN
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 NCBI Summary for CXCL3    This antimicrobial gene encodes a member of the CXC subfamily of chemokines. The encoded protein is a secreted growth factor that signals through the G-protein coupled receptor, CXC receptor 2. This protein plays a role in inflammation and as a chemoattractant for neutrophils. [provided by RefSeq, Sep 2014]
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 UniProt Comments for CXCL3    CXCL3: Ligand for CXCR2. Has chemotactic activity for neutrophils. May play a role in inflammation and exert its effects on endothelial cells in an autocrine fashion. In vitro, the processed form GRO-gamma(5-73) shows a fivefold higher chemotactic activity for neutrophilic granulocytes. Belongs to the intercrine alpha (chemokine CxC) family.

Protein type: Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 4q21

Cellular Component: extracellular space; extracellular region

Molecular Function: chemokine activity; CXCR chemokine receptor binding

Biological Process: G-protein coupled receptor protein signaling pathway; neutrophil chemotaxis; positive regulation of leukocyte chemotaxis; immune response; response to lipopolysaccharide; inflammatory response; regulation of cell proliferation
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 Research Articles on CXCL3    1. our results demonstrate the diverse mechanisms by which CXCL2 and CXCL3 mediate normal and asthmatic airway smooth muscle cell migration
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with CXCL3 elisa kitPathways associated with CXCL3 elisa kit
 Reference Product  PubMed Publications
 CXCL2 elisa kit  >98 publications with CXCL3 and CXCL2
 CXCL1 elisa kit  >96 publications with CXCL3 and CXCL1
 CCL20 elisa kit  >40 publications with CXCL3 and CCL20
 CCL5 elisa kit  >24 publications with CXCL3 and CCL5
 CXCR2 elisa kit  >11 publications with CXCL3 and CXCR2
 CXCR1 elisa kit  >8 publications with CXCL3 and CXCR1
 CXCL16 elisa kit  >6 publications with CXCL3 and CXCL16
 CCL19 elisa kit  >5 publications with CXCL3 and CCL19
 CCR1 elisa kit  >3 publications with CXCL3 and CCR1
 CCL16 elisa kit  >1 publications with CXCL3 and CCL16
 Products by Pathway  Pathway Diagram
 Chemokine Receptors Bind Chemokines Pathway antibodies  Chemokine Receptors Bind Chemokines Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 G Alpha (i) Signalling Events Pathway antibodies  G Alpha (i) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 Legionellosis Pathway antibodies  Legionellosis Pathway Diagram
Diseases associated with CXCL3 elisa kitOrgans/Tissues associated with CXCL3 elisa kit
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >70 publications with CXCL3 and Inflammation
 Necrosis Antibodies  >34 publications with CXCL3 and Necrosis
 Respiratory Tract Diseases Antibodies  >13 publications with CXCL3 and Respiratory Tract Diseases
 Lung Diseases Antibodies  >12 publications with CXCL3 and Lung Diseases
 Immune System Diseases Antibodies  >11 publications with CXCL3 and Immune System Diseases
 Cardiovascular Diseases Antibodies  >8 publications with CXCL3 and Cardiovascular Diseases
 Skin Diseases Antibodies  >7 publications with CXCL3 and Skin Diseases
 Fibrosis Antibodies  >5 publications with CXCL3 and Fibrosis
 Neoplasms, Experimental Antibodies  >4 publications with CXCL3 and Neoplasms, Experimental
 Kidney Diseases Antibodies  >3 publications with CXCL3 and Kidney Diseases
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >61 publications with CXCL3 and Blood
 Lung Antibodies  >15 publications with CXCL3 and Lung
 Intestine Antibodies  >11 publications with CXCL3 and Intestine
 Brain Antibodies  >7 publications with CXCL3 and Brain
 Embryonic Tissue Antibodies  >4 publications with CXCL3 and Embryonic Tissue
 Liver Antibodies  >3 publications with CXCL3 and Liver
 Uterus Antibodies  >2 publications with CXCL3 and Uterus
 Pancreas Antibodies  >1 publications with CXCL3 and Pancreas
 Bladder Antibodies  >1 publications with CXCL3 and Bladder
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