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CLEC4E elisa kit :: Mouse C-type lectin domain family 4 member e, CLEC4E ELISA Kit

Scan QR to view Datasheet Catalog #    MBS7246171
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $455 +1 FREE 8GB USB
96-Strip-Wells  /  $660 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,490 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

C-type lectin domain family 4 member e, CLEC4E, ELISA Kit

★Popular Item★
 Also Known As   

Mouse C-type lectin domain family 4 member e, CLEC4E ELISA Kit

 Product Gene Name   

CLEC4E elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    609962
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 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of CLEC4E. No significant cross-reactivity or interference between CLEC4E and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CLEC4E and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Detection Range    0.5-10ng/mL
 Sensitivity    0.1ng/mL
 Intended Uses    This CLEC4E ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse CLEC4E. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of CLEC4E elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for CLEC4E purchase    MBS7246171 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the C-type lectin domain family 4 member e, CLEC4E, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.5-10ng/mL in biological research samples containing CLEC4E, with an estimated sensitivity of 0.1ng/mL. The ELISA analytical biochemical technique of the MBS7246171 kit is based on CLEC4E antibody-CLEC4E antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CLEC4E antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CLEC4E. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for CLEC4E elisa kit

   Principle of the assay: CLEC4E ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CLEC4E antibody and an CLEC4E-HRP conjugate. The assay sample and buffer are incubated together with CLEC4E-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CLEC4E concentration since CLEC4E from samples and CLEC4E-HRP conjugate compete for the anti-CLEC4E antibody binding site. Since the number of sites is limited, as more sites are occupied by CLEC4E from the sample, fewer sites are left to bind CLEC4E-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CLEC4E concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for CLEC4E elisa kit    Immunology
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 Typical Testing Data/Standard Curve (for reference only) of CLEC4E elisa kit    CLEC4E elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS7246171. Click to request current manual
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NCBI/Uniprot data below describe general gene information for CLEC4E. It may not necessarily be applicable to this product.
 NCBI GI #    7657333
 NCBI GeneID    26253
 NCBI Accession #    NP_055173.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_014358.2 [Other Products]
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 UniProt Secondary Accession #    B2R6Q6 [Other Products]
 UniProt Related Accession #    Q9ULY5 [Other Products]
 Molecular Weight    25,073 Da
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 NCBI Official Full Name    C-type lectin domain family 4 member E
 NCBI Official Synonym Full Names    C-type lectin domain family 4, member E
 NCBI Official Symbol    CLEC4E [Similar Products]
 NCBI Official Synonym Symbols   
MINCLE; CLECSF9
[Similar Products]
 NCBI Protein Information    C-type lectin domain family 4 member E; C-type lectin superfamily member 9; macrophage-inducible C-type lectin; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamily member 9
 UniProt Protein Name    C-type lectin domain family 4 member E
 UniProt Synonym Protein Names   
C-type lectin superfamily member 9; Macrophage-inducible C-type lectin
 Protein Family    C-type lectin domain family
 UniProt Gene Name    CLEC4E [Similar Products]
 UniProt Synonym Gene Names    CLECSF9; MINCLE; UNQ218/PRO244 [Similar Products]
 UniProt Entry Name    CLC4E_HUMAN
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 NCBI Summary for CLEC4E    This gene encodes a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. Members of this family share a common protein fold and have diverse functions, such as cell adhesion, cell-cell signalling, glycoprotein turnover, and roles in inflammation and immune response. The encoded type II transmembrane protein is a downstream target of CCAAT/enhancer binding protein (C/EBP), beta (CEBPB) and may play a role in inflammation. Alternative splice variants have been described but their full-length sequence has not been determined. This gene is closely linked to other CTL/CTLD superfamily members on chromosome 12p13 in the natural killer gene complex region. [provided by RefSeq, Jul 2008]
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 UniProt Comments for CLEC4E    CLEC4E: C-type lectin that functions as cell-surface receptor for a wide variety of ligands such as damaged cells, fungi and mycobacteria. Plays a role in the recognition of pathogenic fungi, such as Candida albicans. The detection of mycobacteria is via trehalose 6,6'-dimycolate (TDM), a cell wall glycolipid. Specifically recognizes alpha-mannose residues on pathogenic fungi of the genus Malassezia. Recognizes also SAP130, a nuclear protein, that is released by dead or dying cells. Transduces signals through an ITAM-containing adapter protein, Fc receptor gamma chain /FCER1G. Induces secretion of inflammatory cytokines through a pathway that depends on SYK, CARD9 and NF-kappa-B.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 12p13.31

Cellular Component: integral to membrane

Molecular Function: receptor activity; carbohydrate binding

Biological Process: immune response; positive regulation of cytokine secretion
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 Research Articles on CLEC4E    1. Results show the molecular mechanism of glycolipid recognition through C-type lectin receptors, which may provide clues to rational design for effective adjuvants.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with CLEC4E elisa kitPathways associated with CLEC4E elisa kit
 Reference Product  PubMed Publications
 CLEC4D elisa kit  >6 publications with CLEC4E and CLEC4D
 TLR2 elisa kit  >2 publications with CLEC4E and TLR2
 CD163 elisa kit  >1 publications with CLEC4E and CD163
 Products by Pathway  Pathway Diagram
 Tuberculosis Pathway antibodies  Tuberculosis Pathway Diagram
 Tuberculosis Pathway antibodies  Tuberculosis Pathway Diagram
Diseases associated with CLEC4E elisa kitOrgans/Tissues associated with CLEC4E elisa kit
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >6 publications with CLEC4E and Inflammation
 Necrosis Antibodies  >3 publications with CLEC4E and Necrosis
 Skin Diseases Antibodies  >2 publications with CLEC4E and Skin Diseases
 Disease Models, Animal Antibodies  >2 publications with CLEC4E and Disease Models, Animal
 Weight Loss Antibodies  >1 publications with CLEC4E and Weight Loss
 Liver Diseases Antibodies  >1 publications with CLEC4E and Liver Diseases
 Atherosclerosis Antibodies  >1 publications with CLEC4E and Atherosclerosis
 Pneumonia Antibodies  >1 publications with CLEC4E and Pneumonia
 Brain Injuries Antibodies  >1 publications with CLEC4E and Brain Injuries
 Lupus Erythematosus, Systemic Antibodies  >1 publications with CLEC4E and Lupus Erythematosus, Systemic
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >5 publications with CLEC4E and Blood
 Connective Tissue Antibodies  >1 publications with CLEC4E and Connective Tissue
 Brain Antibodies  >1 publications with CLEC4E and Brain
 Spleen Antibodies  >1 publications with CLEC4E and Spleen
 Kidney Antibodies  >1 publications with CLEC4E and Kidney
 Lung Antibodies  >1 publications with CLEC4E and Lung
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