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CASP3 elisa kit :: Mouse CASP3 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2505926
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $410 +1 FREE 8GB USB
96-Strip-Wells  /  $490 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   


★Popular Item★
 Also Known As   

Mouse CASP3 (Caspase 3) ELISA Kit

 Product Gene Name   

CASP3 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    600636
 3D Structure    ModBase 3D Structure for P42574
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 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Mouse CASP3. No significant cross-reactivity or interference between Mouse CASP3 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Mouse CASP3 and all the analogues, therefore, cross reaction may still exist.
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 Samples    Serum, Plasma, Biological Fluids
 Assay Type    Sandwich
 Detection Range    0.156-10ng/mL
 Sensitivity    Min: 0.094ng/mL; Max: 10ng/mL
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 Preparation and Storage    Store at 4 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of CASP3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for CASP3 purchase    MBS2505926 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the CASP3, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.156-10ng/mL in biological research samples containing CASP3, with an estimated sensitivity of Min: 0.094ng/mL; Max: 10ng/mL. The ELISA analytical biochemical technique of the MBS2505926 kit is based on CASP3 antibody-CASP3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CASP3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CASP3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for CASP3 elisa kit

   Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CASP3 in Mouse serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse CASP3. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse CASP3 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CASP3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse CASP3. You can calculate the concentration of Mouse CASP3 in the samples by comparing the OD of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of CASP3 elisa kit    CASP3 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2505926. Click to request current manual
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NCBI/Uniprot data below describe general gene information for CASP3. It may not necessarily be applicable to this product.
 NCBI GI #    14790119
 NCBI GeneID    836
 NCBI Accession #    NP_004337.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_004346.3 [Other Products]
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 UniProt Primary Accession #    P42574 [Other Products]
 UniProt Secondary Accession #    Q96AN1; Q96KP2; A8K5M2; D3DP53 [Other Products]
 UniProt Related Accession #    P42574 [Other Products]
 Molecular Weight    31,608 Da
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 NCBI Official Full Name    caspase-3 preproprotein
 NCBI Official Synonym Full Names    caspase 3, apoptosis-related cysteine peptidase
 NCBI Official Symbol    CASP3 [Similar Products]
 NCBI Official Synonym Symbols   
CPP32; SCA-1; CPP32B
[Similar Products]
 NCBI Protein Information    caspase-3; CASP-3; CPP-32; PARP cleavage protease; SREBP cleavage activity 1; apopain; caspase 3, apoptosis-related cysteine protease; cysteine protease CPP32; procaspase3; protein Yama
 UniProt Protein Name    Caspase-3
 UniProt Synonym Protein Names   
Apopain; Cysteine protease CPP32; CPP-32; Protein Yama; SREBP cleavage activity 1; SCA-1
 Protein Family    Caspase
 UniProt Gene Name    CASP3 [Similar Products]
 UniProt Synonym Gene Names    CPP32; CASP-3; CPP-32; SCA-1 [Similar Products]
 UniProt Entry Name    CASP3_HUMAN
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 NCBI Summary for CASP3    This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein. [provided by RefSeq, Jul 2008]
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 UniProt Comments for CASP3    CASP3: Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop- helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage. Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 17 kDa (p17) and a 12 kDa (p12) subunit. Interacts with BIRC6/bruce. Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system. Inhibited by isatin sulfonamides. Belongs to the peptidase C14A family.

Protein type: Motility/polarity/chemotaxis; EC; Protease; Apoptosis

Chromosomal Location of Human Ortholog: 4q34

Cellular Component: nucleoplasm; plasma membrane; cytosol; nucleus

Molecular Function: cyclin-dependent protein kinase inhibitor activity; peptidase activity; protein binding; cysteine-type endopeptidase activity; aspartic-type endopeptidase activity

Biological Process: extracellular matrix organization and biogenesis; nerve growth factor receptor signaling pathway; positive regulation of apoptosis; apoptosis; heart development; negative regulation of activated T cell proliferation; negative regulation of B cell proliferation; regulation of caspase activity; proteolysis; neuron differentiation; extracellular matrix disassembly; sensory perception of sound; B cell homeostasis; positive regulation of neuron apoptosis; response to wounding; erythrocyte differentiation; T cell homeostasis; DNA fragmentation during apoptosis; cell structure disassembly during apoptosis; response to UV; release of cytochrome c from mitochondria; cell fate commitment; negative regulation of cyclin-dependent protein kinase activity; keratinocyte differentiation; neuron apoptosis; induction of apoptosis via death domain receptors; platelet formation; caspase activation via cytochrome c; induction of apoptosis by oxidative stress; response to DNA damage stimulus; negative regulation of apoptosis
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 Research Articles on CASP3    1. Suggest that glucose might attenuate isoflurane-induced caspase-3 activation through a mitochondria-independent reduction in ROS levels and enhancement in ATP levels in neuroglioma cells.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with CASP3 elisa kitPathways associated with CASP3 elisa kit
 Reference Product  PubMed Publications
 CASP9 elisa kit  >6028 publications with CASP3 and CASP9
 CASP8 elisa kit  >4483 publications with CASP3 and CASP8
 XIAP elisa kit  >863 publications with CASP3 and XIAP
 AKT1 elisa kit  >372 publications with CASP3 and AKT1
 PARP1 elisa kit  >352 publications with CASP3 and PARP1
 APAF1 elisa kit  >309 publications with CASP3 and APAF1
 CASP10 elisa kit  >115 publications with CASP3 and CASP10
 BIRC3 elisa kit  >38 publications with CASP3 and BIRC3
 BIRC2 elisa kit  >32 publications with CASP3 and BIRC2
 BCL2L11 elisa kit  >6 publications with CASP3 and BCL2L11
 Products by Pathway  Pathway Diagram
 AGE/RAGE Pathway antibodies  AGE/RAGE Pathway Diagram
 Activation Of DNA Fragmentation Factor Pathway antibodies  Activation Of DNA Fragmentation Factor Pathway Diagram
 Activation Of Caspases Through Apoptosome-mediated Cleavage Pathway antibodies  Activation Of Caspases Through Apoptosome-mediated Cleavage Pathway Diagram
 Alpha6-Beta4 Integrin Signaling Pathway antibodies  Alpha6-Beta4 Integrin Signaling Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Alzheimers Disease Pathway antibodies  Alzheimers Disease Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
Diseases associated with CASP3 elisa kitOrgans/Tissues associated with CASP3 elisa kit
 Disease Name  Pubmed Publications
 Necrosis Antibodies  >5479 publications with CASP3 and Necrosis
 Nervous System Diseases Antibodies  >4089 publications with CASP3 and Nervous System Diseases
 Inflammation Antibodies  >2509 publications with CASP3 and Inflammation
 Liver Diseases Antibodies  >1736 publications with CASP3 and Liver Diseases
 Brain Ischemia Antibodies  >1308 publications with CASP3 and Brain Ischemia
 Heart Diseases Antibodies  >1196 publications with CASP3 and Heart Diseases
 Neoplasms, Experimental Antibodies  >1114 publications with CASP3 and Neoplasms, Experimental
 Breast Neoplasms Antibodies  >1108 publications with CASP3 and Breast Neoplasms
 Kidney Diseases Antibodies  >645 publications with CASP3 and Kidney Diseases
 Fibrosis Antibodies  >609 publications with CASP3 and Fibrosis
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >6062 publications with CASP3 and Brain
 Blood Antibodies  >6045 publications with CASP3 and Blood
 Liver Antibodies  >3224 publications with CASP3 and Liver
 Muscle Antibodies  >2669 publications with CASP3 and Muscle
 Vascular Antibodies  >2590 publications with CASP3 and Vascular
 Lung Antibodies  >2461 publications with CASP3 and Lung
 Heart Antibodies  >2260 publications with CASP3 and Heart
 Kidney Antibodies  >1575 publications with CASP3 and Kidney
 Bone Antibodies  >1346 publications with CASP3 and Bone
 Prostate Antibodies  >1025 publications with CASP3 and Prostate
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