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CCL28 elisa kit :: Mouse CCL28 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS177311
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
96-Strip-Wells  /  $425 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

CCL28, ELISA Kit

 Also Known As   

Mouse CCL28 PicoKine ELISA Kit

 Product Synonym Names    C-C motif chemokine 28; chemokine (C-C motif) ligand 28; CCK 1; CCK1; CCL 28; Chemokine (C-C motif) ligand 28; MEC; MGC71902; Mucosae associated epithelial chemokine; OTTHUMP0000022162; SCYA28; Small inducible cytokine A28; Small inducible cytokine A28 [Precursor]; Small inducible cytokine subfamily A (Cys-Cys), member 28
 Product Gene Name   

CCL28 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 3D Structure    ModBase 3D Structure for Q9JIL2
 Species Reactivity    Mouse
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 Specificity    Natural and recombinant mouse CCL28
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 Samples    Cell culture supernates, serum and plasma (heparin, EDTA)
 Assay Type    Sandwich
 Detection Range    31.2pg/ml-2000pg/ml
 Sensitivity    < 10pg/ml
 Intra-assay Precision    Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
 Inter-assay Precision    Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
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 Preparation and Storage    Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of CCL28 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for CCL28 purchase    MBS177311 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the CCL28, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 31.2pg/ml-2000pg/ml in biological research samples containing CCL28, with an estimated sensitivity of < 10pg/ml. The ELISA analytical biochemical technique of the MBS177311 kit is based on CCL28 antibody-CCL28 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CCL28 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CCL28. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Cell culture supernates, serum and plasma (heparin, EDTA). Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for CCL28 elisa kit

   Principle of the assay: mouse CCL28 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CCL28 has been precoated onto 96-well plates. Standards (E.coli, I23-R130) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL28 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CCL28 amount of sample captured in plate.
Background: CCL28, also known as mucosae-associated epithelial chemokine (MEC), CCK1 and SCYA28, is a chemokine. It is mapped to 5p12. The tumor hypoxia promotes the recruitment of regulatory T (Treg) cells through induction of expression of the chemokine CCL28, which in turn promotes tumor tolerance and angiogenesis. CCL28 regulates the chemotaxis of cells that express the chemokine receptors CCR3 and CCR10. CCL28 has also been implicated in the migration of IgA-expressing cells to the mammary gland, salivary gland, intestine and other mucosal tissues. It has also been shown as a potential antimicrobial agent effective against certain pathogens, such as Gram negative and Gram positive bacteria and the fungus Candida albicans.
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 Typical Testing Data/Standard Curve (for reference only) of CCL28 elisa kit    Mouse CCL28 PicoKine ELISA Kit standard curve
CCL28 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS177311. Click to request current manual
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NCBI/Uniprot data below describe general gene information for CCL28. It may not necessarily be applicable to this product.
 NCBI GI #    12229756
 NCBI GeneID    56838
 NCBI Accession #    Q9JIL2.1 [Other Products]
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 UniProt Primary Accession #    Q9JIL2 [Other Products]
 UniProt Related Accession #    Q9JIL2 [Other Products]
 Molecular Weight    14,570 Da
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 NCBI Official Full Name    C-C motif chemokine 28
 NCBI Official Synonym Full Names    chemokine (C-C motif) ligand 28
 NCBI Official Symbol    Ccl28 [Similar Products]
 NCBI Official Synonym Symbols   
MEC; CCK1; Scya28
[Similar Products]
 NCBI Protein Information    C-C motif chemokine 28; small inducible cytokine A28; small-inducible cytokine A28
 UniProt Protein Name    C-C motif chemokine 28
 UniProt Synonym Protein Names   
Small-inducible cytokine A28
 Protein Family    C-C motif chemokine
 UniProt Gene Name    Ccl28 [Similar Products]
 UniProt Synonym Gene Names    Scya28 [Similar Products]
 UniProt Entry Name    CCL28_MOUSE
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 UniProt Comments for CCL28    CCL28: Chemotactic activity for resting CD4, CD8 T-cells and eosinophils. Binds to CCR3 and CCR10 and induces calcium mobilization in a dose-dependent manner. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Secreted, signal peptide; Chemokine; Motility/polarity/chemotaxis; Secreted

Cellular Component: extracellular space; cell; extracellular region; cytosol

Molecular Function: chemokine activity; cytokine activity

Biological Process: elevation of cytosolic calcium ion concentration; immune response; positive regulation of cell-matrix adhesion; chemotaxis
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 Research Articles on CCL28    1. Plasmid codelivery of murine CCL19 and CCL28 enhanced Ag-specific systemic and mucosal Ab responses to HIV-1 gp140, augmented balanced Th1/Th2 responses, and increased lymphocyte subsets in various lymphoid tissues.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with CCL28 elisa kitPathways associated with CCL28 elisa kit
 Reference Product  PubMed Publications
 Ccr10 elisa kit  >49 publications with CCL28 and Ccr10
 Ccl25 elisa kit  >32 publications with CCL28 and Ccl25
 Ccr3 elisa kit  >17 publications with CCL28 and Ccr3
 Ccl20 elisa kit  >11 publications with CCL28 and Ccl20
 Cxcr5 elisa kit  >2 publications with CCL28 and Cxcr5
 Ccl9 elisa kit  >1 publications with CCL28 and Ccl9
 Ccl6 elisa kit  >1 publications with CCL28 and Ccl6
 Products by Pathway  Pathway Diagram
 Chemokine Receptors Bind Chemokines Pathway antibodies  Chemokine Receptors Bind Chemokines Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 G Alpha (i) Signalling Events Pathway antibodies  G Alpha (i) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
Diseases associated with CCL28 elisa kitOrgans/Tissues associated with CCL28 elisa kit
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >27 publications with CCL28 and Inflammation
 Necrosis Antibodies  >11 publications with CCL28 and Necrosis
 Disease Models, Animal Antibodies  >7 publications with CCL28 and Disease Models, Animal
 Vascular Diseases Antibodies  >6 publications with CCL28 and Vascular Diseases
 Carcinoma Antibodies  >6 publications with CCL28 and Carcinoma
 Adenocarcinoma Antibodies  >6 publications with CCL28 and Adenocarcinoma
 Liver Diseases Antibodies  >5 publications with CCL28 and Liver Diseases
 Hypertension Antibodies  >2 publications with CCL28 and Hypertension
 Kidney Diseases Antibodies  >2 publications with CCL28 and Kidney Diseases
 Neoplasms, Experimental Antibodies  >2 publications with CCL28 and Neoplasms, Experimental
 Organ/Tissue Name  Pubmed Publications
 Intestine Antibodies  >35 publications with CCL28 and Intestine
 Mammary Gland Antibodies  >11 publications with CCL28 and Mammary Gland
 Salivary Gland Antibodies  >10 publications with CCL28 and Salivary Gland
 Uterus Antibodies  >2 publications with CCL28 and Uterus
 Kidney Antibodies  >1 publications with CCL28 and Kidney
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