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CD163 elisa kit :: Mouse CD163 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS177211
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
1x96 Wells  /  $425 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

CD163, ELISA Kit

 Also Known As   

Mouse CD163 PicoKine ELISA Kit

 Product Synonym Names    Scavenger receptor cysteine-rich type 1 protein M130; CD163 molecule; CD_antigen; CD163; Hemoglobin scavenger receptor; M130; MM130; Scavenger receptor cysteine-rich type 1 protein M130
 Product Gene Name   

CD163 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Manual Insert    Download PDF Manual View PDF Manual
 3D Structure    ModBase 3D Structure for Q2VLH6
 Species Reactivity    Mouse
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 Specificity    Natural and recombinant mouse CD163
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 Samples    Cell culture supernates, serum, plasma (heparin)
 Assay Type    Sandwich
 Detection Range    0.78ng/ml-50ng/ml
 Sensitivity    < 20pg/ml
 Intra-assay Precision    Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
 Inter-assay Precision    Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
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 Preparation and Storage    Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay.
 Other Notes    Small volumes of CD163 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for CD163 purchase    MBS177211 is a ready-to-use microwell, strip-or-full plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the CD163, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.78ng/ml-50ng/ml in biological research samples containing CD163, with an estimated sensitivity of < 20pg/ml. The ELISA analytical biochemical technique of the MBS177211 kit is based on CD163 antibody-CD163 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CD163 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CD163. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Cell culture supernates, serum, plasma (heparin). Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for CD163 elisa kit

   Principle of the assay: mouse CD163 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CD163 has been precoated onto 96-well plates. Standards(NSO, V39-T1045) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD163 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CD163 amount of sample captured in plate.
Background: CD163 (Cluster of Differentiation 163) is a human protein encoded by the CD163 gene. It has also been shown to mark cells of monocyte/macrophage lineage. CD163, a member of the scavenger receptor cysteine-rich (SRCR) superfamily, is exclusively expressed by monocytes and macrophages. Using FISH, somatic cell hybrid analysis, and radiation hybrid analysis, CD163 gene was mapped the to chromosome 12p13.3. CD163 is upregulated in a large range of diseases inflammatory diseases including type 2 diabetes, macrophage activation sickness, Tangier's disease, reumatoid arthritis etc.
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 Typical Testing Data/Standard Curve (for reference only) of CD163 elisa kit    CD163 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS177211
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NCBI/Uniprot data below describe general gene information for CD163. It may not necessarily be applicable to this product.
 NCBI GI #    341940294
 NCBI GeneID    93671
 NCBI Accession #    Q2VLH6.2 [Other Products]
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 UniProt Primary Accession #    Q2VLH6 [Other Products]
 UniProt Secondary Accession #    Q2VLH5; Q99MX8; A6H691 [Other Products]
 UniProt Related Accession #    Q2VLH6 [Other Products]
 Molecular Weight    125,292 Da
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 NCBI Official Full Name    Scavenger receptor cysteine-rich type 1 protein M130
 NCBI Official Synonym Full Names    CD163 antigen
 NCBI Official Symbol    Cd163 [Similar Products]
 NCBI Official Synonym Symbols   
CD163v2; CD163v3
[Similar Products]
 NCBI Protein Information    scavenger receptor cysteine-rich type 1 protein M130
 UniProt Protein Name    Scavenger receptor cysteine-rich type 1 protein M130
 UniProt Synonym Protein Names   
CD_antigen: CD163Cleaved into the following chain:Soluble CD163; sCD163
 Protein Family    Scavenger receptor cysteine-rich type 1 protein
 UniProt Gene Name    Cd163 [Similar Products]
 UniProt Synonym Gene Names    M130; sCD163 [Similar Products]
 UniProt Entry Name    C163A_MOUSE
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 UniProt Comments for CD163    CD163: Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Cellular Component: membrane; extracellular region; plasma membrane; integral to membrane

Molecular Function: scavenger receptor activity

Biological Process: receptor-mediated endocytosis; acute-phase response; inflammatory response
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 Research Articles on CD163    1. Data indicate an essential substrate motif for ADAM17-mediated CD163 and proTNF-alpha cleavage in macrophages.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with CD163 elisa kitDiseases associated with CD163 elisa kit
 Products by Pathway  Pathway Diagram
 Binding And Uptake Of Ligands By Scavenger Receptors Pathway antibodies  Binding And Uptake Of Ligands By Scavenger Receptors Pathway Diagram
 Macrophage Markers Pathway antibodies  Macrophage Markers Pathway Diagram
 Scavenging Of Heme From Plasma Pathway antibodies  Scavenging Of Heme From Plasma Pathway Diagram
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >368 publications with CD163 and Inflammation
 Necrosis Antibodies  >165 publications with CD163 and Necrosis
 Nervous System Diseases Antibodies  >105 publications with CD163 and Nervous System Diseases
 Brain Diseases Antibodies  >65 publications with CD163 and Brain Diseases
 Fibrosis Antibodies  >56 publications with CD163 and Fibrosis
 Lung Diseases Antibodies  >51 publications with CD163 and Lung Diseases
 Adenocarcinoma Antibodies  >40 publications with CD163 and Adenocarcinoma
 Heart Diseases Antibodies  >30 publications with CD163 and Heart Diseases
 Kidney Diseases Antibodies  >27 publications with CD163 and Kidney Diseases
 Neoplasms, Experimental Antibodies  >25 publications with CD163 and Neoplasms, Experimental
Organs/Tissues associated with CD163 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Skin Antibodies  >112 publications with CD163 and Skin
 Brain Antibodies  >72 publications with CD163 and Brain
 Lymph Node Antibodies  >61 publications with CD163 and Lymph Node
 Joint Antibodies  >56 publications with CD163 and Joint
 Embryonic Tissue Antibodies  >22 publications with CD163 and Embryonic Tissue
 Bladder Antibodies  >5 publications with CD163 and Bladder
 Mammary Gland Antibodies  >3 publications with CD163 and Mammary Gland
 Vagina Antibodies  >1 publications with CD163 and Vagina
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