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CD248 elisa kit :: Mouse CD248 molecule, endosialin ELISA Kit

Scan QR to view Datasheet Catalog #    MBS931031
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (Limit 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

CD248 molecule, endosialin, ELISA Kit

★Popular Item★
 Also Known As   

Mouse Endosialin, CD248 ELISA Kit

 Product Synonym Names    Mouse Endosialin (CD248) ELISA kit; CD164L1; MGC119478; MGC119479; TEM1; 2610111G01Rik; CD164 sialomucin-like 1; CD248 antigen; endosialin; endosialin; tumor endothelial marker 1; CD248 molecule; endosialin
 Product Gene Name   

CD248 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS931031 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for Q91V98
 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of mouse CD248. No significant cross-reactivity or interference between mouse CD248 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates
 Assay Type    Sandwich
 Detection Range    7.8 1 pg/ml -500 pg/ml.
 Sensitivity    The minimum detectable dose of mouse CD248 is typically less than 1.95 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of CD248 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for CD248 purchase    MBS931031 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the CD248 molecule, endosialin, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 7.8 1 pg/ml -500 pg/ml in biological research samples containing CD248, with an estimated sensitivity of The minimum detectable dose of mouse CD248 is typically less than 1.95 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. The ELISA analytical biochemical technique of the MBS931031 kit is based on CD248 antibody-CD248 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CD248 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CD248. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for CD248 elisa kit

   Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CD248 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CD248 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CD248 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CD248 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of CD248 elisa kit    CD248 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS931031. Click to request current manual
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NCBI/Uniprot data below describe general gene information for CD248. It may not necessarily be applicable to this product.
 NCBI GI #    16923944
 NCBI GeneID    70445
 NCBI Accession #    NP_473383.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_054042.2 [Other Products]
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 UniProt Primary Accession #    Q91V98 [Other Products]
 UniProt Secondary Accession #    Q3UMV6; Q91ZV1 [Other Products]
 UniProt Related Accession #    Q91V98 [Other Products]
 Molecular Weight    81,813 Da
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 NCBI Official Full Name    endosialin
 NCBI Official Synonym Full Names    CD248 antigen, endosialin
 NCBI Official Symbol    Cd248 [Similar Products]
 NCBI Official Synonym Symbols   
Tem1; Cd164l1; AI842296; 2610111G01Rik
[Similar Products]
 NCBI Protein Information    endosialin; CD164 sialomucin-like 1; tumor endothelial marker 1
 UniProt Protein Name    Endosialin
 UniProt Synonym Protein Names   
Tumor endothelial marker 1; CD_antigen: CD248
 Protein Family    Endosialin
 UniProt Gene Name    Cd248 [Similar Products]
 UniProt Synonym Gene Names    Tem1 [Similar Products]
 UniProt Entry Name    CD248_MOUSE
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 UniProt Comments for CD248    CD248: May play a role in tumor angiogenesis. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Cellular Component: membrane; cytoplasm; integral to membrane; plasma membrane

Molecular Function: extracellular matrix binding; calcium ion binding; carbohydrate binding

Biological Process: cell migration; positive regulation of cell proliferation; lymph node development; anatomical structure regression
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 Research Articles on CD248    1. Tem1-TT vaccination elicited CD8+ and/or CD4+ T cell responses against immunodominant TEM1 protein sequences. Prophylactic immunization of animals with Tem1-TT prevented or delayed tumor formation in several tumor models.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with CD248 elisa kitDiseases associated with CD248 elisa kit
 Reference Product  PubMed Publications
 Fap elisa kit  >3 publications with CD248 and Fap
 Antxr1 elisa kit  >1 publications with CD248 and Antxr1
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >8 publications with CD248 and Inflammation
 Disease Models, Animal Antibodies  >6 publications with CD248 and Disease Models, Animal
 Neoplasms, Experimental Antibodies  >6 publications with CD248 and Neoplasms, Experimental
 Breast Neoplasms Antibodies  >3 publications with CD248 and Breast Neoplasms
 Fibrosis Antibodies  >2 publications with CD248 and Fibrosis
 Intestinal Neoplasms Antibodies  >2 publications with CD248 and Intestinal Neoplasms
 Kidney Diseases Antibodies  >2 publications with CD248 and Kidney Diseases
 Necrosis Antibodies  >2 publications with CD248 and Necrosis
 Immune System Diseases Antibodies  >2 publications with CD248 and Immune System Diseases
 Cell Transformation, Neoplastic Antibodies  >1 publications with CD248 and Cell Transformation, Neoplastic
Organs/Tissues associated with CD248 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Embryonic Tissue Antibodies  >10 publications with CD248 and Embryonic Tissue
 Brain Antibodies  >5 publications with CD248 and Brain
 Muscle Antibodies  >4 publications with CD248 and Muscle
 Other Antibodies  >3 publications with CD248 and Other
 Lung Antibodies  >3 publications with CD248 and Lung
 Skin Antibodies  >1 publications with CD248 and Skin
 Spleen Antibodies  >1 publications with CD248 and Spleen
 Liver Antibodies  >1 publications with CD248 and Liver
 Intestine Antibodies  >1 publications with CD248 and Intestine
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