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CXCL5 elisa kit :: Mouse CXCL5/ENA-78 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS176511
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
96-Strip-Wells  /  $390 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

CXCL5/ENA-78, ELISA Kit

★Popular Item★
 Also Known As   

Mouse CXCL5/ENA-78 PicoKine ELISA Kit

 Product Synonym Names    C-X-C motif chemokine 5; chemokine (C-X-C motif) ligand 5; AMCF II; AMCFII; C X C motif chemokine 5; Chemokine (C X C motif) ligand 5; CXCL 5; ENA 78; ENA78; Epithelial derived neutrophil activating protein 78; GCP 2; LIX; Neutrophil activating peptide ENA 78; Neutrophil activating protein 78; SCYB5; Scyb6; Small inducible cytokine B5; Small inducible cytokine subfamily B (Cys X Cys) member 5 (epithelial derived neutrophil activating peptide 78); Small inducible cytokine subfamily B, member 15
 Product Gene Name   

CXCL5 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 3D Structure    ModBase 3D Structure for P50228
 Species Reactivity    Mouse
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 Specificity    Natural and recombinant mouse CXCL5
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 Samples    Cell culture supernates, serum and plasma(heparin1 EDTA, citrate}.
 Assay Type    Quantitative Sandwich
 Sensitivity    < 10pg/ml
 Intra-assay Precision    Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
 Inter-assay Precision    Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
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 Preparation and Storage    Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of CXCL5 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for CXCL5 purchase    MBS176511 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the CXCL5/ENA-78, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CXCL5, with an estimated sensitivity of < 10pg/ml. The ELISA analytical biochemical technique of the MBS176511 kit is based on CXCL5 antibody-CXCL5 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CXCL5 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CXCL5. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Cell culture supernates, serum and plasma(heparin1 EDTA, citrate}. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for CXCL5 elisa kit

   Principle of the Assay: The mouse CXCL5 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CXCL5 has been precoated onto 96-well plates. Standards (Expression system for standard: E.coli, Immunogen sequence: V45-A118) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CXCL5 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CXCL5 amount of sample captured in plate.

Background/Introduction: C-X-C motif chemokine 5 is a protein that in humans encoded by the CXCL5 gene. The protein encoded by this gene, CXCL5 is a small cytokine belonging to the CXC chemokine family that is also known as epithelial-derived neutrophil-activating peptide 78 (ENA-78). It is produced following stimulation of cells with the inflammatory cytokines interleukin-1 or tumor necrosis factor-alpha. Expression of CXCL5 has also been observed in eosinophils, and can be inhibited with the type II interferon IFN-y. This chemokine stimulates the chemotaxis of neutrophils possessing angiogenic properties. It elicits these effects by interacting with the cell surface chemokine receptor CXCR2. The gene for CXCL5 is encoded on four exons and is located on humanchromosome 4 amongst several other CXC chemokine genes. CXCL5 has been implicated in connective tissue remodeling. CXCL5 plays a role in reducing sensitivity to sunburn pain in some subjects, and is a potential target which can be utilized to understand more about pain in other inflammatory conditions like arthritis and cystitis.
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 Typical Testing Data/Standard Curve (for reference only) of CXCL5 elisa kit    Mouse CXCL5/ENA-78 PicoKine ELISA Kit standard curve
CXCL5 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS176511. Click to request current manual
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NCBI/Uniprot data below describe general gene information for CXCL5. It may not necessarily be applicable to this product.
 NCBI GI #    408360041
 NCBI GeneID    20311
 NCBI Accession #    P50228.2 [Other Products]
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 UniProt Primary Accession #    P50228 [Other Products]
 UniProt Secondary Accession #    Q8K3I3 [Other Products]
 UniProt Related Accession #    P50228 [Other Products]
 Molecular Weight    14,235 Da
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 NCBI Official Full Name    C-X-C motif chemokine 5
 NCBI Official Synonym Full Names    chemokine (C-X-C motif) ligand 5
 NCBI Official Symbol    Cxcl5 [Similar Products]
 NCBI Official Synonym Symbols   
LIX; GCP-2; Scyb5; Scyb6; ENA-78; AMCF-II
[Similar Products]
 NCBI Protein Information    C-X-C motif chemokine 5; cytokine LIX; small-inducible cytokine B5; granulocyte chemotactic protein-2; small inducible cytokine B subfamily, member 5; small inducible cytokine subfamily B, member 15
 UniProt Protein Name    C-X-C motif chemokine 5
 UniProt Synonym Protein Names   
Cytokine LIX; Small-inducible cytokine B5Cleaved into the following 2 chains:GCP-2(1-78); GCP-2(9-78)
 Protein Family    C-X-C motif chemokine
 UniProt Gene Name    Cxcl5 [Similar Products]
 UniProt Synonym Gene Names    Scyb5 [Similar Products]
 UniProt Entry Name    CXCL5_MOUSE
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 NCBI Summary for CXCL5    This gene encodes a protein that is a member of the CXC subfamily of chemokines. Chemokines, which recruit and activate leukocytes, are classified by function (inflammatory or homeostatic) or by structure. This protein is proposed to bind the G-protein coupled receptor chemokine (C-X-C motif) receptor 2 to recruit neutrophils and to have homeostatic and inflammatory functions. In mouse, deficiency of this gene is associated with increased lung inflammation that is neutrophil-dependent. [provided by RefSeq, May 2013]
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 UniProt Comments for CXCL5    CXCL6: Chemotactic for neutrophil granulocytes. Belongs to the intercrine alpha (chemokine CxC) family.

Protein type: Secreted, signal peptide; Motility/polarity/chemotaxis; Secreted

Cellular Component: extracellular space; extracellular region

Molecular Function: chemokine activity; CXCR chemokine receptor binding; cytokine activity

Biological Process: G-protein coupled receptor protein signaling pathway; organ regeneration; positive regulation of JAK-STAT cascade; cytokine production; response to lipopolysaccharide; immune response; positive regulation of leukocyte chemotaxis; inflammatory response; chemotaxis; regulation of chemokine production; leukocyte homeostasis; regulation of cell proliferation
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 Research Articles on CXCL5    1. CXCL5 regulates pulmonary responses to infection and plays a central role in conferring clock control of inflammation.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with CXCL5 elisa kitPathways associated with CXCL5 elisa kit
 Reference Product  PubMed Publications
 Cxcl1 elisa kit  >223 publications with CXCL5 and Cxcl1
 Cxcr2 elisa kit  >84 publications with CXCL5 and Cxcr2
 Ccl5 elisa kit  >73 publications with CXCL5 and Ccl5
 Ccl20 elisa kit  >39 publications with CXCL5 and Ccl20
 Cxcl12 elisa kit  >31 publications with CXCL5 and Cxcl12
 Cxcr1 elisa kit  >26 publications with CXCL5 and Cxcr1
 Cxcr3 elisa kit  >15 publications with CXCL5 and Cxcr3
 Cxcl16 elisa kit  >11 publications with CXCL5 and Cxcl16
 Ccr1 elisa kit  >8 publications with CXCL5 and Ccr1
 Ccr2 elisa kit  >6 publications with CXCL5 and Ccr2
 Products by Pathway  Pathway Diagram
 Chemokine Receptors Bind Chemokines Pathway antibodies  Chemokine Receptors Bind Chemokines Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 G Alpha (i) Signalling Events Pathway antibodies  G Alpha (i) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
Diseases associated with CXCL5 elisa kitOrgans/Tissues associated with CXCL5 elisa kit
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >233 publications with CXCL5 and Inflammation
 Necrosis Antibodies  >100 publications with CXCL5 and Necrosis
 Respiratory Tract Diseases Antibodies  >96 publications with CXCL5 and Respiratory Tract Diseases
 Lung Diseases Antibodies  >85 publications with CXCL5 and Lung Diseases
 Disease Models, Animal Antibodies  >62 publications with CXCL5 and Disease Models, Animal
 Carcinoma Antibodies  >59 publications with CXCL5 and Carcinoma
 Cardiovascular Diseases Antibodies  >43 publications with CXCL5 and Cardiovascular Diseases
 Lung Neoplasms Antibodies  >27 publications with CXCL5 and Lung Neoplasms
 Fibrosis Antibodies  >27 publications with CXCL5 and Fibrosis
 Pneumonia Antibodies  >24 publications with CXCL5 and Pneumonia
 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >38 publications with CXCL5 and Liver
 Intestine Antibodies  >34 publications with CXCL5 and Intestine
 Eye Antibodies  >17 publications with CXCL5 and Eye
 Connective Tissue Antibodies  >16 publications with CXCL5 and Connective Tissue
 Embryonic Tissue Antibodies  >14 publications with CXCL5 and Embryonic Tissue
 Spleen Antibodies  >9 publications with CXCL5 and Spleen
 Adipose Tissue Antibodies  >8 publications with CXCL5 and Adipose Tissue
 Pancreas Antibodies  >6 publications with CXCL5 and Pancreas
 Mammary Gland Antibodies  >3 publications with CXCL5 and Mammary Gland
 Vagina Antibodies  >2 publications with CXCL5 and Vagina
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