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CALCB elisa kit :: Mouse Calcitonin gene-related peptide 2 (CALCB) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS7241942
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $455 +1 FREE 8GB USB
96-Strip-Wells  /  $660 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,490 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Calcitonin gene-related peptide 2 (CALCB), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Calcitonin gene-related peptide 2 (CALCB) ELISA Kit

 Product Gene Name   

CALCB elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    114160
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 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of CALCB. No significant cross-reactivity or interference between CALCB and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CALCB and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
 Assay Type    Competitive (Quantitative)
 Sensitivity    1.0 pg/mL.
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of CALCB elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for CALCB purchase    MBS7241942 is a ready-to-use microwell, strip plate Competitive (Quantitative) ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Calcitonin gene-related peptide 2 (CALCB) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CALCB, with an estimated sensitivity of 1.0 pg/mL. The ELISA analytical biochemical technique of the MBS7241942 kit is based on CALCB antibody-CALCB antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CALCB antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CALCB. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for CALCB elisa kit

   Intended Uses: This CALCB ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse CALCB. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: CALCB ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CALCB antibody and an CALCB-HRP conjugate. The assay sample and buffer are incubated together with CALCB-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CALCB concentration since CALCB from samples and CALCB-HRP conjugate compete for the anti-CALCB antibody binding site. Since the number of sites is limited, as more sites are occupied by CALCB from the sample, fewer sites are left to bind CALCB-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CALCB concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for CALCB elisa kit    Neurobiology
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 Typical Testing Data/Standard Curve (for reference only) of CALCB elisa kit    CALCB elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS7241942. Click to request current manual
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NCBI/Uniprot data below describe general gene information for CALCB. It may not necessarily be applicable to this product.
 NCBI GI #    9945304
 NCBI GeneID    797
 NCBI Accession #    NP_000719.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_000728.3 [Other Products]
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 UniProt Secondary Accession #    Q569I0; Q9UCN9; A8K573; D3DQX4 [Other Products]
 UniProt Related Accession #    P10092 [Other Products]
 Molecular Weight    13,706 Da
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 NCBI Official Full Name    calcitonin gene-related peptide 2
 NCBI Official Synonym Full Names    calcitonin-related polypeptide beta
 NCBI Official Symbol    CALCB [Similar Products]
 NCBI Official Synonym Symbols   
CALC2; CGRP2; CGRP-II
[Similar Products]
 NCBI Protein Information    calcitonin gene-related peptide 2; beta-CGRP; calcitonin 2; beta-type CGRP; calcitonin gene-related peptide II
 UniProt Protein Name    Calcitonin gene-related peptide 2
 UniProt Synonym Protein Names   
Beta-type CGRP; Beta-CGRP; Calcitonin gene-related peptide II; CGRP-II
 Protein Family    Calcitonin gene-related peptide
 UniProt Gene Name    CALCB [Similar Products]
 UniProt Synonym Gene Names    CALC2; Beta-CGRP; CGRP-II [Similar Products]
 UniProt Entry Name    CALCB_HUMAN
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 UniProt Comments for CALCB    CALCB: CGRP induces vasodilation. It dilates a variety of vessels including the coronary, cerebral and systemic vasculature. Its abundance in the CNS also points toward a neurotransmitter or neuromodulator role. Belongs to the calcitonin family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 11p15.2-p15.1

Cellular Component: extracellular region

Molecular Function: neuropeptide hormone activity

Biological Process: cellular calcium ion homeostasis; signal transduction
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 Research Articles on CALCB    1. Keratinocyte-derived CGRPbeta may modulate epidermal homeostasis through autocrine/paracrine signaling and may contribute to chronic pain under pathological conditions.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with CALCB elisa kitPathways associated with CALCB elisa kit
 Reference Product  PubMed Publications
 IAPP elisa kit  >1 publications with CALCB and IAPP
 ADM2 elisa kit  >1 publications with CALCB and ADM2
 ADM elisa kit  >1 publications with CALCB and ADM
 Products by Pathway  Pathway Diagram
 Calcitonin-like Ligand Receptors Pathway antibodies  Calcitonin-like Ligand Receptors Pathway Diagram
 Class B/2 (Secretin Family Receptors) Pathway antibodies  Class B/2 (Secretin Family Receptors) Pathway Diagram
 G Alpha (s) Signalling Events Pathway antibodies  G Alpha (s) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 Signal Transduction Pathway antibodies  Signal Transduction Pathway Diagram
 Signaling By GPCR Pathway antibodies  Signaling By GPCR Pathway Diagram
Organs/Tissues associated with CALCB elisa kit
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >2 publications with CALCB and Brain
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