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SCT elisa kit :: Mouse Calcitonin ELISA Kit

Scan QR to view Datasheet Catalog #    MBS095044 SCT elisa kit
Unit / Price
48-Strip-Wells  /  $435 +1 FREE 8GB USB
96-Strip-Wells  /  $600 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,650 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,205 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Calcitonin (SCT), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Calcitonin ELISA Kit

 Product Gene Name   

SCT elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 Chromosome Location    Chromosome: 11; NC_000011.9 (14988215..14993832, complement). Location: 11p15.2
 OMIM    114130
 3D Structure    ModBase 3D Structure for P01258
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 Species Reactivity    Mouse
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 Specificity    No significant cross-reactivity or interference between Mouse SCT and analogues was observed.
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 Samples    Serum, Plasma, Tissue Homogenate, Feces and Urine
 Assay Type    Sandwich
 Detection Range    3.12 pg/ml - 100 pg/ml.
 Sensitivity    1.0 pg/ml.
 Intended Uses    This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated SCT concentrations in Mouse serum, plasma and other body fluids. Using Purified Mouse SCT antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add SCT and SCT antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of SCT in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Intra-assay Precision    Intra-assay CV (%) is less than 15%.
 Inter-assay Precision    Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean 100]
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 Preparation and Storage    Store all reagents at 2-8 degree C
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of SCT elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for SCT purchase    MBS095044 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Calcitonin (SCT) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 3.12 pg/ml - 100 pg/ml in biological research samples containing SCT, with an estimated sensitivity of 1.0 pg/ml. The ELISA analytical biochemical technique of the MBS095044 kit is based on SCT antibody-SCT antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SCT antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SCT. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Tissue Homogenate, Feces and Urine. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for SCT elisa kit

   Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated SCT concentrations in Mouse serum, plasma and other body fluids. Using Purified Mouse SCT antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add SCT and SCT antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of SCT in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Sample Manual Insert of MBS095044. Click to request current manual
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NCBI/Uniprot data below describe general gene information for SCT. It may not necessarily be applicable to this product.
 NCBI GI #    76880484
 NCBI GeneID    796
 NCBI Accession #    NP_001029124.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001033952.2 [Other Products]
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 UniProt Primary Accession #    P01258 [Other Products]
 UniProt Secondary Accession #    Q13935; Q13937; Q52LX7; B7ZL39 [Other Products]
 UniProt Related Accession #    P01258; P06881 [Other Products]
 Molecular Weight    15,466 Da
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 NCBI Official Full Name    calcitonin isoform CT preproprotein
 NCBI Official Synonym Full Names    calcitonin-related polypeptide alpha
 NCBI Official Symbol    CALCA [Similar Products]
 NCBI Official Synonym Symbols   
CT; KC; CGRP; CALC1; CGRP1; CGRP-I
[Similar Products]
 NCBI Protein Information    calcitonin; calcitonin gene-related peptide 1; katacalcin; calcitonin 1; alpha-type CGRP; calcitonin gene-related peptide I; calcitonin/calcitonin-related polypeptide, alpha
 UniProt Protein Name    Calcitonin
 UniProt Synonym Protein Names   
Calcitonin carboxyl-terminal peptide; CCP; PDN-21
 Protein Family    Calcitonin
 UniProt Gene Name    CALCA [Similar Products]
 UniProt Synonym Gene Names    CALC1; CCP [Similar Products]
 UniProt Entry Name    CALC_HUMAN
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 NCBI Summary for SCT    This gene encodes the peptide hormones calcitonin, calcitonin gene-related peptide and katacalcin by tissue-specific alternative RNA splicing of the gene transcripts and cleavage of inactive precursor proteins. Calcitonin is involved in calcium regulation and acts to regulate phosphorus metabolism. Calcitonin gene-related peptide functions as a vasodilator while katacalcin is a calcium-lowering peptide. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Nov 2009]
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 UniProt Comments for SCT    CALCA: Calcitonin causes a rapid but short-lived drop in the level of calcium and phosphate in blood by promoting the incorporation of those ions in the bones. Belongs to the calcitonin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 11p15.2

Cellular Component: extracellular space; cell soma; cytoplasm; extracellular region; terminal button; nucleus

Molecular Function: identical protein binding; protein binding; calcitonin receptor binding; hormone activity; protein complex binding; receptor binding

Biological Process: negative regulation of osteoclast differentiation; elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); positive regulation of interleukin-1 alpha production; vasodilation of artery during baroreceptor response to increased systemic arterial blood pressure; response to pain; positive regulation of vasodilation; G-protein signaling, adenylate cyclase activating pathway; protein amino acid phosphorylation; positive regulation of interleukin-8 production; monocyte chemotaxis; elevation of cytosolic calcium ion concentration; leukocyte adhesion; cell-cell signaling; vasculature development; neuropeptide signaling pathway; negative regulation of smooth muscle contraction; regulation of blood pressure; receptor internalization; positive regulation of cAMP biosynthetic process; negative regulation of ossification; feeding behavior; positive regulation of adenylate cyclase activity; negative regulation of blood pressure; inflammatory response; positive regulation of macrophage differentiation; detection of temperature stimulus involved in sensory perception of pain; negative regulation of bone resorption; negative regulation of neurological process; aging; cytosolic calcium ion homeostasis; regulation of heart rate; adenylate cyclase activation; positive regulation of ossification; regulation of systemic arterial blood pressure by neurological process; G-protein coupled receptor internalization; response to heat; endothelial cell proliferation; regulation of inflammatory response; activation of protein kinase activity; endothelial cell migration; negative regulation of transcription, DNA-dependent; embryo implantation
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 Research Articles on SCT    1. Levels of CGRP protein and mRNA expression in the colonic mucosa of patients are closely associated with ulcerative colitis severity and corroborate traditional indices used to assess the disease.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with SCT elisa kitPathways associated with SCT elisa kit
 Reference Product  PubMed Publications
 RAMP1 elisa kit  >2 publications with SCT and RAMP1
 IAPP elisa kit  >1 publications with SCT and IAPP
 ADM elisa kit  >1 publications with SCT and ADM
 CALCRL elisa kit  >1 publications with SCT and CALCRL
 Products by Pathway  Pathway Diagram
 Amyloids Pathway antibodies  Amyloids Pathway Diagram
 Calcitonin-like Ligand Receptors Pathway antibodies  Calcitonin-like Ligand Receptors Pathway Diagram
 Class B/2 (Secretin Family Receptors) Pathway antibodies  Class B/2 (Secretin Family Receptors) Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Endothelin Pathway antibodies  Endothelin Pathway Diagram
 G Alpha (s) Signalling Events Pathway antibodies  G Alpha (s) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 Myometrial Relaxation And Contraction Pathways antibodies  Myometrial Relaxation And Contraction Pathways Diagram
 Signal Transduction Pathway antibodies  Signal Transduction Pathway Diagram
Diseases associated with SCT elisa kitOrgans/Tissues associated with SCT elisa kit
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >7 publications with SCT and Inflammation
 Pain Antibodies  >3 publications with SCT and Pain
 Hypertension Antibodies  >3 publications with SCT and Hypertension
 Kidney Diseases Antibodies  >3 publications with SCT and Kidney Diseases
 Neurogenic Inflammation Antibodies  >2 publications with SCT and Neurogenic Inflammation
 Migraine Disorders Antibodies  >2 publications with SCT and Migraine Disorders
 Drug Toxicity Antibodies  >2 publications with SCT and Drug Toxicity
 Headache Antibodies  >2 publications with SCT and Headache
 Edema Antibodies  >2 publications with SCT and Edema
 Necrosis Antibodies  >1 publications with SCT and Necrosis
 Organ/Tissue Name  Pubmed Publications
 Thyroid Antibodies  >9 publications with SCT and Thyroid
 Brain Antibodies  >6 publications with SCT and Brain
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