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cTn1 elisa kit :: Mouse Cardiac Troponin 1 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS727843
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells-(Competitive)  /  $455 +1 FREE 8GB USB
48-Strip-Wells-(Sandwich)  /  $455 +1 FREE 8GB USB
96-Strip-Wells-(Competitive)  /  $660 +1 FREE 8GB USB
96-Strip-Wells-(Sandwich)  /  $660 +1 FREE 8GB USB
5x96-Strip-Wells-(Competitive)  /  $2,925 +3 FREE 8GB USB
5x96-Strip-Wells-(Sandwich)  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells-(Competitive)  /  $5,490 +6 FREE 8GB USB
10x96-Strip-Wells-(Sandwich)  /  $5,490 +6 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Cardiac Troponin 1 (cTn1), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Cardiac Troponin 1 ELISA Kit

 Product Gene Name   

cTn1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Competitive ELISA Manual Insert    Competitive ELISA PDF Manual
 Request for Current Manual Insert    Request Current Manual
 3D Structure    ModBase 3D Structure for Q6FGX2
 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of cTnI. No significant cross-reactivity or interference between cTnI and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between cTnI and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive or Sandwich
 Detection Range    50-1000pg/mL
 Sensitivity    1.0pg/mL
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of cTn1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for cTn1 purchase    MBS727843 is a ready-to-use microwell, strip plate Competitive or Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Cardiac Troponin 1 (cTn1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 50-1000pg/mL in biological research samples containing cTn1, with an estimated sensitivity of 1.0pg/mL. The ELISA analytical biochemical technique of the MBS727843 kit is based on cTn1 antibody-cTn1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect cTn1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, cTn1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for cTn1 elisa kit

   Intended Uses: This cTnI ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse cTnI. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: cTnI ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-cTnI antibody and an cTnI-HRP conjugate. The assay sample and buffer are incubated together with cTnI-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the cTnI concentration since cTnI from samples and cTnI-HRP conjugate compete for the anti-cTnI antibody binding site. Since the number of sites is limited, as more sites are occupied by cTnI from the sample, fewer sites are left to bind cTnI-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The cTnI concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for cTn1 elisa kit    Cardiovascular
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 Typical Testing Data/Standard Curve (for reference only) of cTn1 elisa kit    cTn1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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NCBI/Uniprot data below describe general gene information for cTn1. It may not necessarily be applicable to this product.
 NCBI GI #    49456923
 NCBI GeneID    7137
 NCBI Accession #    CAG46782.1 [Other Products]
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 UniProt Primary Accession #    Q6FGX2 [Other Products]
 UniProt Related Accession #    P19429 [Other Products]
 Molecular Weight    24,008 Da
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 NCBI Official Full Name    TNNI3
 NCBI Official Synonym Full Names    troponin I type 3 (cardiac)
 NCBI Official Symbol    TNNI3 [Similar Products]
 NCBI Official Synonym Symbols   
[Similar Products]
 NCBI Protein Information    troponin I, cardiac muscle; cardiomyopathy, dilated 2A (autosomal recessive)
 UniProt Protein Name    TNNI3 protein
 UniProt Synonym Protein Names   
TNNI3 protein; Troponin I type 3 (Cardiac); Troponin I, cardiac; Troponin I, cardiac variant
 UniProt Gene Name    TNNI3 [Similar Products]
 UniProt Entry Name    Q6FGX2_HUMAN
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 NCBI Summary for cTn1    Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM). [provided by RefSeq, Jul 2008]
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 UniProt Comments for cTn1    TNNI3: Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. Binds to actin and tropomyosin. Interacts with TRIM63. Interacts with STK4/MST1. Belongs to the troponin I family.

Protein type: Actin-binding; Motility/polarity/chemotaxis; Motor

Chromosomal Location of Human Ortholog: 19q13.4

Cellular Component: sarcomere; troponin complex; cytosol

Molecular Function: troponin T binding; troponin C binding; protein domain specific binding; protein binding; metal ion binding; calcium channel inhibitor activity; actin binding; calcium-dependent protein binding; protein kinase binding

Biological Process: regulation of smooth muscle contraction; cellular calcium ion homeostasis; heart contraction; regulation of systemic arterial blood pressure by ischemic conditions; heart development; ventricular cardiac muscle morphogenesis; vasculogenesis; negative regulation of ATPase activity; muscle filament sliding; cardiac muscle contraction

Disease: Cardiomyopathy, Dilated, 1ff; Cardiomyopathy, Familial Restrictive, 1; Cardiomyopathy, Familial Hypertrophic, 7; Cardiomyopathy, Dilated, 2a
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 Research Articles on cTn1    1. Cardiac TnI release is common before Norwood and BDG operations; then during the treatment protocol for hypoplastic left heart syndrome, cTnI release resolves.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with cTn1 elisa kitDiseases associated with cTn1 elisa kit
 Products by Pathway  Pathway Diagram
 Adrenergic Signaling In Cardiomyocytes Pathway antibodies  Adrenergic Signaling In Cardiomyocytes Pathway Diagram
 Adrenergic Signaling In Cardiomyocytes Pathway antibodies  Adrenergic Signaling In Cardiomyocytes Pathway Diagram
 Cardiac Progenitor Differentiation Pathway antibodies  Cardiac Progenitor Differentiation Pathway Diagram
 Cardiac Muscle Contraction Pathway antibodies  Cardiac Muscle Contraction Pathway Diagram
 Cardiac Muscle Contraction Pathway antibodies  Cardiac Muscle Contraction Pathway Diagram
 Dilated Cardiomyopathy Pathway antibodies  Dilated Cardiomyopathy Pathway Diagram
 Dilated Cardiomyopathy Pathway antibodies  Dilated Cardiomyopathy Pathway Diagram
 Hypertrophic Cardiomyopathy (HCM) Pathway antibodies  Hypertrophic Cardiomyopathy (HCM) Pathway Diagram
 Hypertrophic Cardiomyopathy (HCM) Pathway antibodies  Hypertrophic Cardiomyopathy (HCM) Pathway Diagram
 Muscle Contraction Pathway antibodies  Muscle Contraction Pathway Diagram
 Disease Name  Pubmed Publications
 Heart Diseases Antibodies  >71 publications with cTn1 and Heart Diseases
 Cardiomyopathies Antibodies  >63 publications with cTn1 and Cardiomyopathies
 Cardiomyopathy, Hypertrophic, Familial Antibodies  >25 publications with cTn1 and Cardiomyopathy, Hypertrophic, Familial
 Cardiomyopathy, Dilated Antibodies  >19 publications with cTn1 and Cardiomyopathy, Dilated
 Cardiomyopathy, Restrictive Antibodies  >8 publications with cTn1 and Cardiomyopathy, Restrictive
 Nervous System Diseases Antibodies  >5 publications with cTn1 and Nervous System Diseases
 Hypertension Antibodies  >4 publications with cTn1 and Hypertension
 Cardiomyopathy, Familial Restrictive, 1 Antibodies  >3 publications with cTn1 and Cardiomyopathy, Familial Restrictive, 1
 Disease Models, Animal Antibodies  >2 publications with cTn1 and Disease Models, Animal
Organs/Tissues associated with cTn1 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Heart Antibodies  >60 publications with cTn1 and Heart
 Embryonic Tissue Antibodies  >2 publications with cTn1 and Embryonic Tissue
 Lung Antibodies  >2 publications with cTn1 and Lung
 Skin Antibodies  >1 publications with cTn1 and Skin
 Uterus Antibodies  >1 publications with cTn1 and Uterus
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