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cTnT elisa kit :: Mouse Cardiac Troponin T ELISA Kit

Scan QR to view Datasheet Catalog #    MBS726068
 Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $440 +1 FREE 8GB USB
96-Strip-Wells  /  $640 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Cardiac Troponin T (cTnT), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Cardiac Troponin T ELISA Kit

 Product Gene Name   

cTnT elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    612422
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 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of cTnT. No significant cross-reactivity or interference between cTnT and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between cTnT and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
 Assay Type    Quantitative Competitive
 Sensitivity    0.1 ng/mL.
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of cTnT elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for cTnT purchase    MBS726068 is a ready-to-use microwell, strip plate Quantitative Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Cardiac Troponin T (cTnT) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing cTnT, with an estimated sensitivity of 0.1 ng/mL. The ELISA analytical biochemical technique of the MBS726068 kit is based on cTnT antibody-cTnT antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect cTnT antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, cTnT. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for cTnT elisa kit

   Intended Uses: This cTnT ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse cTnT. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: cTnT ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-cTnT antibody and an cTnT-HRP conjugate. The assay sample and buffer are incubated together with cTnT-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the cTnT concentration since cTnT from samples and cTnT-HRP conjugate compete for the anti-cTnT antibody binding site. Since the number of sites is limited, as more sites are occupied by cTnT from the sample, fewer sites are left to bind cTnT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The cTnT concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for cTnT elisa kit    Cardiovascular
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  Typical Testing Data/Standard Curve (for reference only) of cTnT elisa kit    cTnT elisa kit  Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS726068. Click to request current manual
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NCBI/Uniprot data below describe general gene information for cTnT. It may not necessarily be applicable to this product.
 NCBI GI #    15290516
 NCBI GeneID    7139
 NCBI Accession #    AAK92231.1 [Other Products]
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 UniProt Secondary Accession #    O60214; Q99596; Q99597; Q9BUF6; Q9UM96; A2TDB9; A8K3K6 [Other Products]
 UniProt Related Accession #    P45379 [Other Products]
 Molecular Weight    30,681 Da
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 NCBI Official Full Name    cardiac troponin T
 NCBI Official Synonym Full Names    troponin T type 2 (cardiac)
 NCBI Official Symbol    TNNT2 [Similar Products]
 NCBI Official Synonym Symbols   
CMH2; RCM3; TnTC; cTnT; CMD1D; CMPD2; LVNC6
[Similar Products]
 NCBI Protein Information    troponin T, cardiac muscle; troponin T2, cardiac; cardiomyopathy, hypertrophic 2; cardiomyopathy, dilated 1D (autosomal dominant)
 UniProt Protein Name    Troponin T, cardiac muscle
 UniProt Synonym Protein Names   
Cardiac muscle troponin T; cTnT
 UniProt Gene Name    TNNT2 [Similar Products]
 UniProt Synonym Gene Names    TnTc; cTnT [Similar Products]
 UniProt Entry Name    TNNT2_HUMAN
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 NCBI Summary for cTnT    The protein encoded by this gene is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in this gene have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Transcripts for this gene undergo alternative splicing that results in many tissue-specific isoforms, however, the full-length nature of some of these variants has not yet been determined. [provided by RefSeq, Jul 2008]
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 UniProt Comments for cTnT    TNNT2: Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. Heart. The fetal heart shows a greater expression in the atrium than in the ventricle, while the adult heart shows a greater expression in the ventricle than in the atrium. Isoform 6 predominates in normal adult heart. Isoforms 1, 7 and 8 are expressed in fetal heart. Isoform 7 is also expressed in failing adult heart. Belongs to the troponin T family. 11 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Motor

Chromosomal Location of Human Ortholog: 1q32

Cellular Component: sarcomere; troponin complex; cytosol; striated muscle thin filament

Molecular Function: troponin C binding; structural constituent of cytoskeleton; troponin I binding; ATPase activity; tropomyosin binding; actin binding

Biological Process: metabolic process; atrial cardiac muscle morphogenesis; positive regulation of ATPase activity; regulation of muscle contraction; ventricular cardiac muscle morphogenesis; sarcomere organization; response to calcium ion; negative regulation of ATPase activity; regulation of heart contraction; muscle filament sliding

Disease: Cardiomyopathy, Familial Restrictive, 3; Cardiomyopathy, Dilated, 1d; Cardiomyopathy, Familial Hypertrophic, 2
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 Research Articles on cTnT    1. Data indicate that increased plasma high sensitivity cardiac troponin (hs-cTnT) levels occur in a significant proportion of adult survivors of childhood acute leukaemias.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with cTnT elisa kitPathways associated with cTnT elisa kit
 Reference Product  PubMed Publications
 MYBPC3 elisa kit  >72 publications with cTnT and MYBPC3
 TNNI3 elisa kit  >56 publications with cTnT and TNNI3
 TPM1 elisa kit  >36 publications with cTnT and TPM1
 MYL2 elisa kit  >22 publications with cTnT and MYL2
 MYL3 elisa kit  >19 publications with cTnT and MYL3
 TNNC1 elisa kit  >13 publications with cTnT and TNNC1
 TTN elisa kit  >8 publications with cTnT and TTN
 TNNI2 elisa kit  >1 publications with cTnT and TNNI2
 TNNI1 elisa kit  >1 publications with cTnT and TNNI1
 Products by Pathway  Pathway Diagram
 Adrenergic Signaling In Cardiomyocytes Pathway antibodies  Adrenergic Signaling In Cardiomyocytes Pathway Diagram
 Adrenergic Signaling In Cardiomyocytes Pathway antibodies  Adrenergic Signaling In Cardiomyocytes Pathway Diagram
 Cardiac Progenitor Differentiation Pathway antibodies  Cardiac Progenitor Differentiation Pathway Diagram
 Cardiac Muscle Contraction Pathway antibodies  Cardiac Muscle Contraction Pathway Diagram
 Cardiac Muscle Contraction Pathway antibodies  Cardiac Muscle Contraction Pathway Diagram
 Dilated Cardiomyopathy Pathway antibodies  Dilated Cardiomyopathy Pathway Diagram
 Dilated Cardiomyopathy Pathway antibodies  Dilated Cardiomyopathy Pathway Diagram
 Hypertrophic Cardiomyopathy (HCM) Pathway antibodies  Hypertrophic Cardiomyopathy (HCM) Pathway Diagram
 Hypertrophic Cardiomyopathy (HCM) Pathway antibodies  Hypertrophic Cardiomyopathy (HCM) Pathway Diagram
 Muscle Contraction Pathway antibodies  Muscle Contraction Pathway Diagram
Diseases associated with cTnT elisa kitOrgans/Tissues associated with cTnT elisa kit
 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >154 publications with cTnT and Cardiovascular Diseases
 Heart Diseases Antibodies  >151 publications with cTnT and Heart Diseases
 Cardiomyopathies Antibodies  >116 publications with cTnT and Cardiomyopathies
 Cardiomyopathy, Hypertrophic Antibodies  >102 publications with cTnT and Cardiomyopathy, Hypertrophic
 Cardiomyopathy, Hypertrophic, Familial Antibodies  >47 publications with cTnT and Cardiomyopathy, Hypertrophic, Familial
 Cardiomyopathy, Dilated Antibodies  >44 publications with cTnT and Cardiomyopathy, Dilated
 Cardiomegaly Antibodies  >41 publications with cTnT and Cardiomegaly
 Hypertrophy Antibodies  >39 publications with cTnT and Hypertrophy
 Heart Failure Antibodies  >35 publications with cTnT and Heart Failure
 Death, Sudden, Cardiac Antibodies  >33 publications with cTnT and Death, Sudden, Cardiac
 Organ/Tissue Name  Pubmed Publications
 Heart Antibodies  >118 publications with cTnT and Heart
 Muscle Antibodies  >94 publications with cTnT and Muscle
 Blood Antibodies  >49 publications with cTnT and Blood
 Embryonic Tissue Antibodies  >16 publications with cTnT and Embryonic Tissue
 Kidney Antibodies  >7 publications with cTnT and Kidney
 Lung Antibodies  >4 publications with cTnT and Lung
 Brain Antibodies  >4 publications with cTnT and Brain
 Connective Tissue Antibodies  >2 publications with cTnT and Connective Tissue
 Liver Antibodies  >2 publications with cTnT and Liver
 Eye Antibodies  >1 publications with cTnT and Eye
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