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CASP1 elisa kit :: Mouse Caspase 1 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS761619
Unit / Price
48-Strip-Wells  /  $280 +1 FREE 8GB USB
96-Strip-Wells  /  $375 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,615 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,015 +3 FREE 8GB USB
15x96-Strip-Wells  /  $4,285 +5 FREE 8GB USB
25x96-Strip-Wells  /  $6,715 +8 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Caspase 1 (CASP1), ELISA Kit

 Also Known As   

Mouse Caspase 1 ELISA Kit

 Product Synonym Names    CASP1/Caspase-1/ICE/CASP-1/ICEP45/IL-1 beta-converting enzyme/IL-1BC/IL1BCCASP1 nirs variant 1/IL1BCE/IL1B-convertase/interleukin 1-B converting enzyme/interleukin 1-beta convertase
 Product Gene Name   

CASP1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 Species Reactivity    Mouse
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 Samples    Serum, plasma and other biological fluids.
 Assay Type    Sandwich ELISA, Double Antibody
 Detection Range    31.25-2000pg/ml
 Sensitivity    18.75pg/ml
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 Preparation and Storage    Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of CASP1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for CASP1 purchase    MBS761619 is a ready-to-use microwell, strip plate Sandwich, Double Antibody ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Caspase 1 (CASP1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 31.25-2000pg/ml in biological research samples containing CASP1, with an estimated sensitivity of 18.75pg/ml. The ELISA analytical biochemical technique of the MBS761619 kit is based on CASP1 antibody-CASP1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CASP1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CASP1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for CASP1 elisa kit

   Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Mouse CASP1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse CASP1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse CASP1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse CASP1 can be calculated.
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 Typical Testing Data of CASP1 elisa kit    CASP1 elisa kit Typical Testing Data image
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 Typical Standard Curve of CASP1 elisa kit    CASP1 elisa kit Typical Standard Curve image
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Sample Manual Insert of MBS761619. Click to request current manual
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NCBI/Uniprot data below describe general gene information for CASP1. It may not necessarily be applicable to this product.
 NCBI GI #    31542341
 NCBI GeneID    25166
 NCBI Accession #    NP_036894.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_012762.2 [Other Products]
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 UniProt Related Accession #    P43527 [Other Products]
 Molecular Weight    45,576 Da
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 NCBI Official Full Name    caspase-1
 NCBI Official Synonym Full Names    caspase 1
 NCBI Official Symbol    Casp1 [Similar Products]
 NCBI Official Synonym Symbols   
Ice; Il1bc
[Similar Products]
 NCBI Protein Information    caspase-1
 UniProt Protein Name    Caspase-1
 UniProt Synonym Protein Names   
Interleukin-1 beta convertase; IL-1BC
 UniProt Gene Name    Casp1 [Similar Products]
 UniProt Synonym Gene Names    Il1bc; Il1bce; CASP-1; IL-1BC; ICE; IL-1 beta-converting enzyme [Similar Products]
 UniProt Entry Name    CASP1_RAT
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 NCBI Summary for CASP1    apoptotic protease that may play a role in inflammation and apoptosis in male sex organs [RGD, Feb 2006]
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 UniProt Comments for CASP1    CASP1: Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis (By similarity)

Protein type: Protease; Apoptosis; EC
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 Research Articles on CASP1    1. The expression of caspase-1-activated cytokines IL-1beta and IL-18 plays a pivotal role in acute renal injury in rats with experimental severe acute pancreatitis. Caspase-1 inhibition improves renal function effectively.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with CASP1 elisa kitPathways associated with CASP1 elisa kit
 Reference Product  PubMed Publications
 Il18 elisa kit  >604 publications with CASP1 and Il18
 Pycard elisa kit  >262 publications with CASP1 and Pycard
 Nlrc4 elisa kit  >209 publications with CASP1 and Nlrc4
 Il1b elisa kit  >128 publications with CASP1 and Il1b
 Nod2 elisa kit  >72 publications with CASP1 and Nod2
 Naip5 elisa kit  >38 publications with CASP1 and Naip5
 Nlrp1b elisa kit  >21 publications with CASP1 and Nlrp1b
 Nfkb1 elisa kit  >5 publications with CASP1 and Nfkb1
 Nlrp1a elisa kit  >4 publications with CASP1 and Nlrp1a
 Nfkb2 elisa kit  >2 publications with CASP1 and Nfkb2
 Products by Pathway  Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
 Androgen Receptor Signaling Pathway antibodies  Androgen Receptor Signaling Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 Cytosolic DNA-sensing Pathway antibodies  Cytosolic DNA-sensing Pathway Diagram
 Cytosolic DNA-sensing Pathway antibodies  Cytosolic DNA-sensing Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Influenza A Pathway antibodies  Influenza A Pathway Diagram
 Influenza A Pathway antibodies  Influenza A Pathway Diagram
Organs/Tissues associated with CASP1 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >275 publications with CASP1 and Lung
 Spleen Antibodies  >112 publications with CASP1 and Spleen
 Eye Antibodies  >107 publications with CASP1 and Eye
 Colon Antibodies  >81 publications with CASP1 and Colon
 Adipose Tissue Antibodies  >45 publications with CASP1 and Adipose Tissue
 Small Intestine Antibodies  >25 publications with CASP1 and Small Intestine
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