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| Product Name
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Catalase, ELISA Kit ★Popular Item★
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| Also Known As
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Mouse Catalase ELISA Kit
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| Product Gene Name
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[Similar Products]
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| Research Use Only
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For Research Use Only. Not for use in diagnostic procedures.
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| Sample Manual Insert
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| Request for Current Manual Insert
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| OMIM
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gene 614097
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| Species Reactivity
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Mouse
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| Samples
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Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
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| Assay Type
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Competitive
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| Detection Range
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5.0-100ng/mL
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| Sensitivity
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1.0ng/mL
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| Intended Uses
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This CAT ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse CAT. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
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| Preparation and Storage
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Store all reagents at 2-8 degree C.
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| Sample Preparation
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We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
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| Product Note
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Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
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| Other Notes
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Small volumes of CAT elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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| Searchable Terms for CAT purchase
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MBS728474 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Catalase, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 5.0-100ng/mL in biological research samples containing CAT, with an estimated sensitivity of 1.0ng/mL. The ELISA analytical biochemical technique of the MBS728474 kit is based on CAT antibody-CAT antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CAT antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CAT. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for CAT elisa kit
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Principle of the Assay: CAT ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for CAT. Standards or samples are then added to the microtiter plate wells and CAT if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of CAT present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for CAT are added to each well to "sandwich" the CAT immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain CAT and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CAT concentration in each sample is interpolated from this standard curve.
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| Typical Testing Data/Standard Curve (for reference only) of CAT elisa kit
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| Sample Manual Insert of MBS728474. Click to request current manual
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| NCBI/Uniprot data below describe general gene information for CAT. It may not necessarily be applicable to this product. |
| NCBI GI #
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4557014
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| NCBI GeneID
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847
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| NCBI Accession #
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NP_001743.1
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[Other Products]
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| NCBI GenBank Nucleotide #
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NM_001752.3
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[Other Products]
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| UniProt Secondary Accession #
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Q2M1U4; Q4VXX5; Q9BWT9; Q9UC85; A8K6C0; B2RCZ9; D3DR07
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[Other Products]
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| UniProt Related Accession #
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P04040
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[Other Products]
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| Molecular Weight
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59,756 Da
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| NCBI Official Full Name
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catalase
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| NCBI Official Synonym Full Names
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catalase
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| NCBI Official Symbol
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CAT
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[Similar Products]
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| NCBI Protein Information
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catalase
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| UniProt Protein Name
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Catalase
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| Protein Family
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Catalase
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| UniProt Gene Name
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CAT
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[Similar Products]
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| UniProt Entry Name
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CATA_HUMAN
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| NCBI Summary for CAT
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This gene encodes catalase, a key antioxidant enzyme in the bodies defense against oxidative stress. Catalase is a heme enzyme that is present in the peroxisome of nearly all aerobic cells. Catalase converts the reactive oxygen species hydrogen peroxide to water and oxygen and thereby mitigates the toxic effects of hydrogen peroxide. Oxidative stress is hypothesized to play a role in the development of many chronic or late-onset diseases such as diabetes, asthma, Alzheimer's disease, systemic lupus erythematosus, rheumatoid arthritis, and cancers. Polymorphisms in this gene have been associated with decreases in catalase activity but, to date, acatalasemia is the only disease known to be caused by this gene. [provided by RefSeq, Oct 2009]
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| UniProt Comments for CAT
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Catalase: Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells. Homotetramer. Belongs to the catalase family.
Protein type: Oxidoreductase; Apoptosis; Amino Acid Metabolism - tryptophan; EC 1.11.1.6; Energy Metabolism - methane; Hydrolase; Endoplasmic reticulum; Mitochondrial
Chromosomal Location of Human Ortholog: 11p13
Cellular Component: Golgi apparatus; peroxisomal membrane; peroxisomal matrix; focal adhesion; membrane; intracellular membrane-bound organelle; endoplasmic reticulum; lysosome; plasma membrane; mitochondrial intermembrane space; peroxisome; cytosol
Molecular Function: antioxidant activity; oxidoreductase activity, acting on peroxide as acceptor; protein homodimerization activity; enzyme binding; catalase activity; metal ion binding; heme binding; NADP binding; receptor binding; aminoacylase activity
Biological Process: cholesterol metabolic process; hemoglobin metabolic process; nucleobase, nucleoside and nucleotide metabolic process; purine base metabolic process; activation of NF-kappaB transcription factor; protein homotetramerization; positive regulation of phosphoinositide 3-kinase cascade; osteoblast differentiation; response to vitamin E; response to reactive oxygen species; triacylglycerol metabolic process; response to hyperoxia; UV protection; ureteric bud development; hydrogen peroxide catabolic process; inhibition of NF-kappaB transcription factor; positive regulation of cell division; aerobic respiration; response to hypoxia; purine nucleotide catabolic process; protein tetramerization; aging; negative regulation of apoptosis
Disease: Acatalasemia
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| Research Articles on CAT
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1. The TT genotype of catalase was more frequent among the asthmatic patients than in healthy Slovak children. The -262 C/T promoter polymorphism may contribute to asthma and oxidative damage.
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| Precautions
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All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
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| Disclaimer
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While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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| Products associated with CAT elisa kit | Pathways associated with CAT elisa kit |
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| Diseases associated with CAT elisa kit | Organs/Tissues associated with CAT elisa kit |
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ELISA Kit 
