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CLIC4 elisa kit :: Mouse Chloride intracellular channel protein 4 (CLIC4) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS7221119
Typical Testing Data/Standard Curve
Unit / Price
48-Strip-Wells  /  $440 +1 FREE 8GB USB
96-Strip-Wells  /  $640 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Chloride intracellular channel protein 4 (CLIC4), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Chloride intracellular channel protein 4 (CLIC4) ELISA Kit

 Product Gene Name   

CLIC4 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    606536
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 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of CLIC4. No significant cross-reactivity or interference between CLIC4 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CLIC4 and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Detection Range    0.5-10g/mL
 Sensitivity    0.1ng/mL
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of CLIC4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for CLIC4 purchase    MBS7221119 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Chloride intracellular channel protein 4 (CLIC4) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.5-10g/mL in biological research samples containing CLIC4, with an estimated sensitivity of 0.1ng/mL. The ELISA analytical biochemical technique of the MBS7221119 kit is based on CLIC4 antibody-CLIC4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CLIC4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CLIC4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for CLIC4 elisa kit

   Intended Uses: This CLIC4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse CLIC4. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay||CLIC4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CLIC4 antibody and an CLIC4-HRP conjugate. The assay sample and buffer are incubated together with CLIC4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CLIC4 concentration since CLIC4 from samples and CLIC4-HRP conjugate compete for the anti-CLIC4 antibody binding site. Since the number of sites is limited, as more sites are occupied by CLIC4 from the sample, fewer sites are left to bind CLIC4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CLIC4 concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for CLIC4 elisa kit    Signal Transduction
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 Typical Testing Data/Standard Curve of CLIC4 elisa kit    CLIC4 elisa kit Typical Testing Data/Standard Curve image
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Sample Manual Insert of MBS7221119. Click to request current manual
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NCBI/Uniprot data below describe general gene information for CLIC4. It may not necessarily be applicable to this product.
 NCBI GI #    7330335
 NCBI GeneID    25932
 NCBI Accession #    NP_039234.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_013943.2 [Other Products]
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 UniProt Secondary Accession #    Q9UFW9; Q9UQJ6 [Other Products]
 UniProt Related Accession #    Q9Y696 [Other Products]
 Molecular Weight    28,772 Da
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 NCBI Official Full Name    chloride intracellular channel protein 4
 NCBI Official Synonym Full Names    chloride intracellular channel 4
 NCBI Official Symbol    CLIC4 [Similar Products]
 NCBI Official Synonym Symbols   
H1; huH1; p64H1; CLIC4L; MTCLIC
[Similar Products]
 NCBI Protein Information    chloride intracellular channel protein 4; chloride intracellular channel 4 like; intracellular chloride ion channel protein p64H1
 UniProt Protein Name    Chloride intracellular channel protein 4
 UniProt Synonym Protein Names   
Intracellular chloride ion channel protein p64H1
 Protein Family    Chloride intracellular channel protein
 UniProt Gene Name    CLIC4 [Similar Products]
 UniProt Entry Name    CLIC4_HUMAN
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 NCBI Summary for CLIC4    Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. Chloride intracellular channel 4 (CLIC4) protein, encoded by the CLIC4 gene, is a member of the p64 family; the gene is expressed in many tissues and exhibits a intracellular vesicular pattern in Panc-1 cells (pancreatic cancer cells). [provided by RefSeq, Jul 2008]
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 UniProt Comments for CLIC4    CLIC4: Can insert into membranes and form poorly selective ion channels that may also transport chloride ions. Channel activity depends on the pH. Membrane insertion seems to be redox-regulated and may occur only under oxydizing conditions. Promotes cell- surface expression of HRH3. Has alternate cellular functions like a potential role in angiogenesis or in maintaining apical- basolateral membrane polarity during mitosis and cytokinesis. Could also promote endothelial cell proliferation and regulate endothelial morphogenesis (tubulogenesis). Belongs to the chloride channel CLIC family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 1p36.11

Cellular Component: centrosome; microvillus; cytoplasmic vesicle membrane; cell surface; nuclear matrix; mitochondrion; intercellular junction; cytosol; actin cytoskeleton; microtubule cytoskeleton; perinuclear region of cytoplasm; apical part of cell; cytoplasm; plasma membrane; intracellular; midbody

Molecular Function: protein binding; chloride channel activity; voltage-gated ion channel activity

Biological Process: apoptosis; multicellular organism growth; establishment and/or maintenance of apical/basal cell polarity; chloride transport; keratinocyte differentiation; vacuolar acidification; fertilization; branching morphogenesis of a tube; endothelial cell morphogenesis; angiogenesis; cell differentiation; negative regulation of cell migration; regulation of cytoskeleton organization and biogenesis
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 Research Articles on CLIC4    1. Increased CLIC4 expression is an early manifestation and mediator of endothelial dysfunction in pulmonary hypertension.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with CLIC4 elisa kitDiseases associated with CLIC4 elisa kit
 Reference Product  PubMed Publications
 PPM1A elisa kit  >1 publications with CLIC4 and PPM1A
 HIVEP2 elisa kit  >1 publications with CLIC4 and HIVEP2
 BMPR2 elisa kit  >1 publications with CLIC4 and BMPR2
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >6 publications with CLIC4 and Inflammation
 Necrosis Antibodies  >5 publications with CLIC4 and Necrosis
 Nervous System Diseases Antibodies  >4 publications with CLIC4 and Nervous System Diseases
 Hypertension Antibodies  >3 publications with CLIC4 and Hypertension
 Breast Neoplasms Antibodies  >2 publications with CLIC4 and Breast Neoplasms
 Disease Models, Animal Antibodies  >2 publications with CLIC4 and Disease Models, Animal
 Neoplasms, Experimental Antibodies  >2 publications with CLIC4 and Neoplasms, Experimental
 Immune System Diseases Antibodies  >1 publications with CLIC4 and Immune System Diseases
 Proteinuria Antibodies  >1 publications with CLIC4 and Proteinuria
 Fibrosis Antibodies  >1 publications with CLIC4 and Fibrosis
Organs/Tissues associated with CLIC4 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >11 publications with CLIC4 and Brain
 Vascular Antibodies  >10 publications with CLIC4 and Vascular
 Blood Antibodies  >9 publications with CLIC4 and Blood
 Muscle Antibodies  >8 publications with CLIC4 and Muscle
 Lung Antibodies  >7 publications with CLIC4 and Lung
 Skin Antibodies  >6 publications with CLIC4 and Skin
 Bone Antibodies  >5 publications with CLIC4 and Bone
 Heart Antibodies  >4 publications with CLIC4 and Heart
 Eye Antibodies  >4 publications with CLIC4 and Eye
 Liver Antibodies  >3 publications with CLIC4 and Liver
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