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ChAT elisa kit :: Mouse Choline acetyltransferase ELISA Kit

Scan QR to view Datasheet Catalog #    MBS724080
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $455 +1 FREE 8GB USB
96-Strip-Wells  /  $660 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,490 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Choline acetyltransferase (ChAT), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Choline acetyltransferase ELISA Kit

 Product Gene Name   

ChAT elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    gene 254210
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 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of ChAT. No significant cross-reactivity or interference between ChAT and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ChAT and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Detection Range    1.0-25ng/mL
 Sensitivity    0.1ng/mL
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of ChAT elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for ChAT purchase    MBS724080 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Choline acetyltransferase (ChAT) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 1.0-25ng/mL in biological research samples containing ChAT, with an estimated sensitivity of 0.1ng/mL. The ELISA analytical biochemical technique of the MBS724080 kit is based on ChAT antibody-ChAT antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ChAT antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ChAT. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for ChAT elisa kit

   Intended Uses: This ChAT ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse ChAT.

Principle of the Assay: ChAT ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ChAT antibody and an ChAT-HRP conjugate. The assay sample and buffer are incubated together with ChAT-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ChAT concentration since ChAT from samples and ChAT-HRP conjugate compete for the anti-ChAT antibody binding site. Since the number of sites is limited, as more sites are occupied by ChAT from the sample, fewer sites are left to bind ChAT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ChAT concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for ChAT elisa kit    Neurobiology
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 Typical Testing Data/Standard Curve (for reference only) of ChAT elisa kit    ChAT elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS724080. Click to request current manual
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NCBI/Uniprot data below describe general gene information for ChAT. It may not necessarily be applicable to this product.
 NCBI GI #    553229
 NCBI GeneID    1103
 NCBI Accession #    AAC37590.1 [Other Products]
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 UniProt Secondary Accession #    Q16488; Q9BQ23; Q9BQ35; Q9BQE1; A2BDF4 [Other Products]
 UniProt Related Accession #    P28329 [Other Products]
 Molecular Weight    70,394 Da
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 NCBI Official Full Name    choline acetyltransferase, partial
 NCBI Official Synonym Full Names    choline O-acetyltransferase
 NCBI Official Symbol    CHAT [Similar Products]
 NCBI Official Synonym Symbols   
CMS1A; CMS1A2; CHOACTASE
[Similar Products]
 NCBI Protein Information    choline O-acetyltransferase; choline acetylase; acetyl CoA:choline O-acetyltransferase
 UniProt Protein Name    Choline O-acetyltransferase
 Protein Family    (Z)-3-hexen-1-ol acetyltransferase
 UniProt Gene Name    CHAT [Similar Products]
 UniProt Synonym Gene Names    CHOACTase; ChAT; Choline acetylase [Similar Products]
 UniProt Entry Name    CLAT_HUMAN
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 NCBI Summary for ChAT    This gene encodes an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer's disease. Polymorphisms in this gene have been associated with Alzheimer's disease and mild cognitive impairment. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform. [provided by RefSeq, May 2010]
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 UniProt Comments for ChAT    CHAT: an enzyme that catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses. Four alternative splice variants have been described.

Protein type: Acetyltransferase; EC 2.3.1.6; Lipid Metabolism - glycerophospholipid

Chromosomal Location of Human Ortholog: 10q11.2

Cellular Component: cell soma; mitochondrion; axon; cytoplasm; nucleus; cytosol

Molecular Function: choline O-acetyltransferase activity

Biological Process: synaptic transmission; rhythmic behavior; rhythmic excitation; muscle development; neurotransmitter secretion; phospholipid metabolic process; glycerophospholipid biosynthetic process; dendrite development; phosphatidylcholine biosynthetic process; neuromuscular synaptic transmission; establishment of synaptic specificity at neuromuscular junction; adult walking behavior; neurotransmitter biosynthetic process

Disease: Myasthenic Syndrome, Congenital, Associated With Episodic Apnea
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 Research Articles on ChAT    1. The results demonstrate that human NSCs over-expressing ChAT improve cognitive function and physical activity of aging mice.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with ChAT elisa kitPathways associated with ChAT elisa kit
 Reference Product  PubMed Publications
 ACHE elisa kit  >874 publications with ChAT and ACHE
 NGF elisa kit  >591 publications with ChAT and NGF
 SLC18A3 elisa kit  >128 publications with ChAT and SLC18A3
 SLC5A7 elisa kit  >7 publications with ChAT and SLC5A7
 VAMP2 elisa kit  >1 publications with ChAT and VAMP2
 KIFAP3 elisa kit  >1 publications with ChAT and KIFAP3
 Products by Pathway  Pathway Diagram
 Acetylcholine Neurotransmitter Release Cycle Pathway antibodies  Acetylcholine Neurotransmitter Release Cycle Pathway Diagram
 Acetylcholine Synthesis Pathway antibodies  Acetylcholine Synthesis Pathway Diagram
 Biogenic Amine Synthesis Pathway antibodies  Biogenic Amine Synthesis Pathway Diagram
 Cholinergic Synapse Pathway antibodies  Cholinergic Synapse Pathway Diagram
 Glycerophospholipid Biosynthesis Pathway antibodies  Glycerophospholipid Biosynthesis Pathway Diagram
 Glycerophospholipid Metabolism Pathway antibodies  Glycerophospholipid Metabolism Pathway Diagram
 Glycerophospholipid Metabolism Pathway antibodies  Glycerophospholipid Metabolism Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Lipids And Lipoproteins Pathway antibodies  Metabolism Of Lipids And Lipoproteins Pathway Diagram
 Neuronal System Pathway antibodies  Neuronal System Pathway Diagram
Diseases associated with ChAT elisa kitOrgans/Tissues associated with ChAT elisa kit
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >2488 publications with ChAT and Nervous System Diseases
 Brain Diseases Antibodies  >1340 publications with ChAT and Brain Diseases
 Neurodegenerative Diseases Antibodies  >1023 publications with ChAT and Neurodegenerative Diseases
 Neurobehavioral Manifestations Antibodies  >883 publications with ChAT and Neurobehavioral Manifestations
 Disease Models, Animal Antibodies  >534 publications with ChAT and Disease Models, Animal
 Nerve Degeneration Antibodies  >433 publications with ChAT and Nerve Degeneration
 Cardiovascular Diseases Antibodies  >308 publications with ChAT and Cardiovascular Diseases
 Movement Disorders Antibodies  >268 publications with ChAT and Movement Disorders
 Memory Disorders Antibodies  >260 publications with ChAT and Memory Disorders
 Cognition Disorders Antibodies  >242 publications with ChAT and Cognition Disorders
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >5870 publications with ChAT and Brain
 Eye Antibodies  >545 publications with ChAT and Eye
 Testis Antibodies  >13 publications with ChAT and Testis
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