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CSHL1 elisa kit :: Mouse Chorionic somatomammotropin hormone-like 1 (CSHL1) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS7218958
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $440 +1 FREE 8GB USB
96-Strip-Wells  /  $640 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
 
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 Product Name   

Chorionic somatomammotropin hormone-like 1 (CSHL1), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Chorionic somatomammotropin hormone-like 1 (CSHL1) ELISA Kit

 Product Gene Name   

CSHL1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    603515
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 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of CSHL1. No significant cross-reactivity or interference between CSHL1and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CSHL1and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
 Assay Type    Quantitative Competitive
 Sensitivity    1.0 ng/mL.
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of CSHL1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for CSHL1 purchase    MBS7218958 is a ready-to-use microwell, strip plate Quantitative Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Chorionic somatomammotropin hormone-like 1 (CSHL1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CSHL1, with an estimated sensitivity of 1.0 ng/mL. The ELISA analytical biochemical technique of the MBS7218958 kit is based on CSHL1 antibody-CSHL1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CSHL1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CSHL1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for CSHL1 elisa kit

   Intended Uses: This CSHL1ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse CSHL1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: CSHL1ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CSHL1antibody and an CSHL1-HRP conjugate. The assay sample and buffer are incubated together with CSHL1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CSHL1concentration since CSHL1from samples and CSHL1-HRP conjugate compete for the anti-CSHL1antibody binding site. Since the number of sites is limited, as more sites are occupied by CSHL1from the sample, fewer sites are left to bind CSHL1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CSHL1concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for CSHL1 elisa kit    Signal Transduction
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 Typical Testing Data/Standard Curve (for reference only) of CSHL1 elisa kit    CSHL1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS7218958. Click to request current manual
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NCBI/Uniprot data below describe general gene information for CSHL1. It may not necessarily be applicable to this product.
 NCBI GI #    12545376
 NCBI GeneID    1444
 NCBI Accession #    NP_072101.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_022579.1 [Other Products]
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 UniProt Secondary Accession #    Q0VDB2; D3DU26; D3DU27 [Other Products]
 UniProt Related Accession #    Q14406 [Other Products]
 Molecular Weight    14,875 Da
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 NCBI Official Full Name    chorionic somatomammotropin hormone-like 1 isoform 1
 NCBI Official Synonym Full Names    chorionic somatomammotropin hormone-like 1
 NCBI Official Symbol    CSHL1 [Similar Products]
 NCBI Official Synonym Symbols   
CSL; CS-5; CSHP1; hCS-L
[Similar Products]
 NCBI Protein Information    chorionic somatomammotropin hormone-like 1; growth hormone cluster; chorionic somatomammotropin CS-5
 UniProt Protein Name    Chorionic somatomammotropin hormone-like 1
 UniProt Synonym Protein Names   
Lactogen-like
 Protein Family    Chorionic somatomammotropin hormone
 UniProt Gene Name    CSHL1 [Similar Products]
 UniProt Synonym Gene Names    CSHP1; CSL; Chorionic somatomammotropin-like [Similar Products]
 UniProt Entry Name    CSHL_HUMAN
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 NCBI Summary for CSHL1    The protein encoded by this gene is a member of the somatotropin/prolactin family of hormones which play an important role in growth control. The gene, along with four other related genes, is located at the growth hormone locus on chromosome 17 where they are interspersed in the same transcriptional orientation; an arrangement which is thought to have evolved by a series of gene duplications. Although the five genes share a remarkably high degree of sequence identity, they are expressed selectively in different tissues. This particular family member is expressed in placental villi, although it was originally thought to be a pseudogene. In fact, alternative splicing suggests that the majority of the transcripts would be unable to express a secreted protein. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
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 UniProt Comments for CSHL1    CSHL1: May be a novel gestational hormone required to compensate for absence of other members of the GH/CS cluster during gestation. Belongs to the somatotropin/prolactin family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 17q24.2

Cellular Component: extracellular region

Molecular Function: metal ion binding; hormone activity
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 Research Articles on CSHL1    1. Observational study of gene-disease association, gene-environment interaction, and pharmacogenomic / toxicogenomic. (HuGE Navigator)
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Diseases associated with CSHL1 elisa kitOrgans/Tissues associated with CSHL1 elisa kit
 Disease Name  Pubmed Publications
 Prenatal Injuries Antibodies  >1 publications with CSHL1 and Prenatal Injuries
 Organ/Tissue Name  Pubmed Publications
 Placenta Antibodies  >2 publications with CSHL1 and Placenta
 Blood Antibodies  >1 publications with CSHL1 and Blood
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