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CD8 elisa kit :: Mouse Cluster of Differentiation 8 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS733399
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $455 +1 FREE 8GB USB
96-Strip-Wells  /  $660 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,490 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Cluster of Differentiation 8 (CD8), ELISA Kit

 Also Known As   

Mouse Cluster of Differentiation 8 ELISA Kit

 Product Gene Name   

CD8 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    186730
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 Species Reactivity    Mouse
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Detection Range    0.5-10ng/mL
 Sensitivity    0.1ng/mL
 Intended Uses    This CD8 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Moue CD8. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of CD8 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for CD8 purchase    MBS733399 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Cluster of Differentiation 8 (CD8) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.5-10ng/mL in biological research samples containing CD8, with an estimated sensitivity of 0.1ng/mL. The ELISA analytical biochemical technique of the MBS733399 kit is based on CD8 antibody-CD8 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CD8 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CD8. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for CD8 elisa kit

   Principle of the Assay: CD8 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CD8 antibody and an CD8-HRP conjugate. The assay sample and buffer are incubated together with CD8-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CD8 concentration since CD8 from samples and CD8-HRP conjugate compete for the anti-CD8 antibody binding site. Since the number of sites is limited, as more sites are occupied by CD8 from the sample, fewer sites are left to bind CD8-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CD8 concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for CD8 elisa kit    Immunology
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 Typical Testing Data/Standard Curve (for reference only) of CD8 elisa kit    CD8 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS733399. Click to request current manual
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NCBI/Uniprot data below describe general gene information for CD8. It may not necessarily be applicable to this product.
 NCBI GI #    178673
 NCBI GeneID    926
 NCBI Accession #    AAA52283.1 [Other Products]
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 UniProt Secondary Accession #    P14860; P14861; Q15980; Q496E0; Q496E1; Q496E2; Q9UDB4; Q9UDB5; Q9UDB6; Q9UDB7; Q9UDB8 [Other Products]
 UniProt Related Accession #    P10966 [Other Products]
 Molecular Weight    22,237 Da
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 NCBI Official Full Name    CD8 antigen, partial
 NCBI Official Synonym Full Names    CD8b molecule
 NCBI Official Symbol    CD8B [Similar Products]
 NCBI Official Synonym Symbols   
LY3; P37; LEU2; LYT3; CD8B1
[Similar Products]
 NCBI Protein Information    T-cell surface glycoprotein CD8 beta chain; CD8 antigen, beta polypeptide 1 (p37); T lymphocyte surface glycoprotein beta chain
 UniProt Protein Name    T-cell surface glycoprotein CD8 beta chain
 UniProt Synonym Protein Names   
CD_antigen: CD8b
 UniProt Gene Name    CD8B [Similar Products]
 UniProt Synonym Gene Names    CD8B1 [Similar Products]
 UniProt Entry Name    CD8B_HUMAN
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 NCBI Summary for CD8    The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 beta chain isoforms. Multiple alternatively spliced transcript variants encoding distinct membrane associated or secreted isoforms have been described. A pseudogene, also located on chromosome 2, has been identified. [provided by RefSeq, May 2010]
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 UniProt Comments for CD8    CD8B: Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 2p12

Cellular Component: T cell receptor complex; early endosome membrane; integral to plasma membrane; extracellular region; plasma membrane; external side of plasma membrane

Molecular Function: protein binding; MHC class I protein binding; coreceptor activity

Biological Process: regulation of immune response; T cell activation; viral reproduction; immune response; regulation of defense response to virus by virus; transmembrane receptor protein tyrosine kinase signaling pathway
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 Research Articles on CD8    1. CREMalpha orchestrates epigenetic remodeling of the CD8A,B through the recruitment of DNA methyltransferase (DNMT) 3a and histone methyltransferase G9a.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with CD8 elisa kitPathways associated with CD8 elisa kit
 Reference Product  PubMed Publications
 CD8A elisa kit  >19 publications with CD8 and CD8A
 CD4 elisa kit  >16 publications with CD8 and CD4
 CD3E elisa kit  >1 publications with CD8 and CD3E
 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Antigen Processing And Presentation Pathway antibodies  Antigen Processing And Presentation Pathway Diagram
 Antigen Processing And Presentation Pathway antibodies  Antigen Processing And Presentation Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Downstream Signaling In Naive CD8+ T Cells Pathway antibodies  Downstream Signaling In Naive CD8+ T Cells Pathway Diagram
 HIV Infection Pathway antibodies  HIV Infection Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
Diseases associated with CD8 elisa kitOrgans/Tissues associated with CD8 elisa kit
 Disease Name  Pubmed Publications
 Disease Models, Animal Antibodies  >2 publications with CD8 and Disease Models, Animal
 Necrosis Antibodies  >1 publications with CD8 and Necrosis
 Liver Diseases Antibodies  >1 publications with CD8 and Liver Diseases
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >27 publications with CD8 and Blood
 Thymus Antibodies  >6 publications with CD8 and Thymus
 Lymph Node Antibodies  >4 publications with CD8 and Lymph Node
 Trachea Antibodies  >2 publications with CD8 and Trachea
 Intestine Antibodies  >1 publications with CD8 and Intestine
 Lung Antibodies  >1 publications with CD8 and Lung
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