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F9 elisa kit :: Mouse Coagulation Factor IX ELISA Kit

Scan QR to view Datasheet Catalog #    MBS005749 F9 elisa kit
Unit / Price
48-Strip-Wells  /  $470 +1 FREE 8GB USB
96-Strip-Wells  /  $680 +1 FREE 8GB USB
5x96-Strip-Wells  /  $3,100 +3 FREE 8GB USB
10x96-Strip-Wells  /  $6,095 +7 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Coagulation Factor IX (F9), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Coagulation Factor IX ELISA Kit

 Product Gene Name   

F9 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 Chromosome Location    Chromosome: X; NC_000023.10 (138612895..138645617). Location: Xq27.1-q27.2
 OMIM    300746
 3D Structure    ModBase 3D Structure for P00740
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 Species Reactivity    Mouse
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 Specificity    No significant cross-reactivity or interference between Mouse F9 and analogues was observed.
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 Samples    Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids
 Assay Type    Sandwich
 Detection Range    6.25 ug/ml - 200 ug/ml.
 Sensitivity    1.0 ug/ml.
 Intended Uses    This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated F9 concentrations in Mouse serum, plasma and other body fluids. Using Purified Mouse F9 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add F9 and F9 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of F9 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Intra-assay Precision    Intra-assay CV (%) is less than 15%.
 Inter-assay Precision    Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]
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 Preparation and Storage    Store all reagents at 2-8 degree C
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of F9 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for F9 purchase    MBS005749 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Coagulation Factor IX (F9) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 6.25 ug/ml - 200 ug/ml in biological research samples containing F9, with an estimated sensitivity of 1.0 ug/ml. The ELISA analytical biochemical technique of the MBS005749 kit is based on F9 antibody-F9 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect F9 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, F9. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for F9 elisa kit

   Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated F9 concentrations in Mouse serum, plasma and other body fluids. Using Purified Mouse F9 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add F9 and F9 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of F9 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Sample Manual Insert of MBS005749. Click to request current manual
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NCBI/Uniprot data below describe general gene information for F9. It may not necessarily be applicable to this product.
 NCBI GI #    4503649
 NCBI GeneID    2158
 NCBI Accession #    NP_000124.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_000133.3 [Other Products]
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 UniProt Primary Accession #    P00740 [Other Products]
 UniProt Secondary Accession #    Q5JYJ8; A8K9N4; F2RM36 [Other Products]
 UniProt Related Accession #    P00740 [Other Products]
 Molecular Weight    51,778 Da
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 NCBI Official Full Name    coagulation factor IX preproprotein
 NCBI Official Synonym Full Names    coagulation factor IX
 NCBI Official Symbol    F9 [Similar Products]
 NCBI Official Synonym Symbols   
FIX; P19; PTC; HEMB; THPH8
[Similar Products]
 NCBI Protein Information    coagulation factor IX; F9 p22; FIX F9; factor 9; factor IX F9; Christmas factor; plasma thromboplastic component; plasma thromboplastin component
 UniProt Protein Name    Coagulation factor IX
 UniProt Synonym Protein Names   
Christmas factor; Plasma thromboplastin component
 Protein Family    Coagulation factor
 UniProt Gene Name    F9 [Similar Products]
 UniProt Synonym Gene Names    PTC [Similar Products]
 UniProt Entry Name    FA9_HUMAN
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 NCBI Summary for F9    This gene encodes vitamin K-dependent coagulation factor IX that circulates in the blood as an inactive zymogen. This factor is converted to an active form by factor XIa, which excises the activation peptide and thus generates a heavy chain and a light chain held together by one or more disulfide bonds. The role of this activated factor IX in the blood coagulation cascade is to activate factor X to its active form through interactions with Ca+2 ions, membrane phospholipids, and factor VIII. Alterations of this gene, including point mutations, insertions and deletions, cause factor IX deficiency, which is a recessive X-linked disorder, also called hemophilia B or Christmas disease. [provided by RefSeq, Jul 2008]
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 UniProt Comments for F9    F9: Factor IX is a vitamin K-dependent plasma protein that participates in the intrinsic pathway of blood coagulation by converting factor X to its active form in the presence of Ca(2+) ions, phospholipids, and factor VIIIa. Defects in F9 are the cause of recessive X-linked hemophilia B (HEMB); also known as Christmas disease. Mutations in position 43 (Oxford-3, San Dimas) and 46 (Cambridge) prevents cleavage of the propeptide, mutation in position 93 (Alabama) probably fails to bind to cell membranes, mutation in position 191 (Chapel-Hill) or in position 226 (Nagoya OR Hilo) prevent cleavage of the activation peptide. Defects in F9 are the cause of thrombophilia due to factor IX defect (THPH8). A hemostatic disorder characterized by a tendency to thrombosis. Belongs to the peptidase S1 family.

Protein type: Secreted; Protease; Secreted, signal peptide; EC 3.4.21.22

Chromosomal Location of Human Ortholog: Xq27.1-q27.2

Cellular Component: Golgi lumen; endoplasmic reticulum lumen; plasma membrane; extracellular region

Molecular Function: serine-type endopeptidase activity; calcium ion binding

Biological Process: blood coagulation, extrinsic pathway; cellular protein metabolic process; post-translational protein modification; blood coagulation; proteolysis; peptidyl-glutamic acid carboxylation; blood coagulation, intrinsic pathway

Disease: Hemophilia B; Thrombophilia, X-linked, Due To Factor Ix Defect; Coumarin Resistance
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 Research Articles on F9    1. Results demonstrate the role of plasticity in regulating FIXa function with respect to discrimination of extended substrate sequences.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with F9 elisa kitPathways associated with F9 elisa kit
 Reference Product  PubMed Publications
 F10 elisa kit  >22 publications with F9 and F10
 F12 elisa kit  >19 publications with F9 and F12
 SERPINC1 elisa kit  >2 publications with F9 and SERPINC1
 GGCX elisa kit  >1 publications with F9 and GGCX
 Products by Pathway  Pathway Diagram
 Blood Clotting Cascade Pathway antibodies  Blood Clotting Cascade Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Extrinsic Pathway antibodies  Extrinsic Pathway Diagram
 Formation Of Fibrin Clot (Clotting Cascade) Pathway antibodies  Formation Of Fibrin Clot (Clotting Cascade) Pathway Diagram
 Gamma-carboxylation Of Protein Precursors Pathway antibodies  Gamma-carboxylation Of Protein Precursors Pathway Diagram
 Gamma-carboxylation, Transport, And Amino-terminal Cleavage Of Proteins Pathway antibodies  Gamma-carboxylation, Transport, And Amino-terminal Cleavage Of Proteins Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 Intrinsic Pathway antibodies  Intrinsic Pathway Diagram
Diseases associated with F9 elisa kitOrgans/Tissues associated with F9 elisa kit
 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >860 publications with F9 and Carcinoma
 Cell Transformation, Neoplastic Antibodies  >75 publications with F9 and Cell Transformation, Neoplastic
 Hemophilia B Antibodies  >68 publications with F9 and Hemophilia B
 Immune System Diseases Antibodies  >37 publications with F9 and Immune System Diseases
 Liver Diseases Antibodies  >27 publications with F9 and Liver Diseases
 Liver Neoplasms Antibodies  >23 publications with F9 and Liver Neoplasms
 Adenocarcinoma Antibodies  >23 publications with F9 and Adenocarcinoma
 Mental Disorders Antibodies  >22 publications with F9 and Mental Disorders
 Drug Toxicity Antibodies  >21 publications with F9 and Drug Toxicity
 Cardiovascular Diseases Antibodies  >19 publications with F9 and Cardiovascular Diseases
 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >98 publications with F9 and Liver
 Uncharacterized Tissue Antibodies  >1 publications with F9 and Uncharacterized Tissue
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