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F9 elisa kit :: Mouse Coagulation Factor IX ELISA Kit

Scan QR to view Datasheet Catalog #    MBS721320
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $455 +1 FREE 8GB USB
96-Strip-Wells  /  $660 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,490 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Coagulation Factor IX (F9), ELISA Kit

 Also Known As   

Mouse Coagulation Factor IX ELISA Kit

 Product Gene Name   

F9 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    306900
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 Species Reactivity    Mouse
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Detection Range    0.5-10ng/mL
 Sensitivity    0.1ng/mL
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of F9 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for F9 purchase    MBS721320 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Coagulation Factor IX (F9) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.5-10ng/mL in biological research samples containing F9, with an estimated sensitivity of 0.1ng/mL. The ELISA analytical biochemical technique of the MBS721320 kit is based on F9 antibody-F9 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect F9 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, F9. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for F9 elisa kit

   For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Intended Use This F9 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Moue F9. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

PRINCIPLE OF THE ASSAY F9 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-F9 antibody and an F9-HRP conjugate. The assay sample and buffer are incubated together with F9-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the F9 concentration since F9 from samples and F9-HRP conjugate compete for the anti-F9 antibody binding site. Since the number of sites is limited, as more sites are occupied by F9 from the sample, fewer sites are left to bind F9-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The F9 concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for F9 elisa kit    Cardiovascular
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 Typical Testing Data/Standard Curve (for reference only) of F9 elisa kit    F9 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS721320. Click to request current manual
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NCBI/Uniprot data below describe general gene information for F9. It may not necessarily be applicable to this product.
 NCBI GI #    326684024
 NCBI GeneID    2158
 NCBI Accession #    CCA61112.1 [Other Products]
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 UniProt Secondary Accession #    Q5FBE1; Q5JYJ8; A8K9N4; F2RM36 [Other Products]
 UniProt Related Accession #    P00740 [Other Products]
 Molecular Weight    47,618 Da
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 NCBI Official Full Name    coagulation factor IX
 NCBI Official Synonym Full Names    coagulation factor IX
 NCBI Official Symbol    F9 [Similar Products]
 NCBI Official Synonym Symbols   
FIX; P19; PTC; HEMB; THPH8
[Similar Products]
 NCBI Protein Information    coagulation factor IX; F9 p22; FIX F9; factor 9; factor IX F9; Christmas factor; plasma thromboplastic component; plasma thromboplastin component
 UniProt Protein Name    Coagulation factor IX
 UniProt Synonym Protein Names   
Christmas factor; Plasma thromboplastin component; PTC
 Protein Family    Coagulation factor
 UniProt Gene Name    F9 [Similar Products]
 UniProt Synonym Gene Names    PTC [Similar Products]
 UniProt Entry Name    FA9_HUMAN
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 NCBI Summary for F9    This gene encodes vitamin K-dependent coagulation factor IX that circulates in the blood as an inactive zymogen. This factor is converted to an active form by factor XIa, which excises the activation peptide and thus generates a heavy chain and a light chain held together by one or more disulfide bonds. The role of this activated factor IX in the blood coagulation cascade is to activate factor X to its active form through interactions with Ca+2 ions, membrane phospholipids, and factor VIII. Alterations of this gene, including point mutations, insertions and deletions, cause factor IX deficiency, which is a recessive X-linked disorder, also called hemophilia B or Christmas disease. [provided by RefSeq, Jul 2008]
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 UniProt Comments for F9    F9: Factor IX is a vitamin K-dependent plasma protein that participates in the intrinsic pathway of blood coagulation by converting factor X to its active form in the presence of Ca(2+) ions, phospholipids, and factor VIIIa. Defects in F9 are the cause of recessive X-linked hemophilia B (HEMB); also known as Christmas disease. Mutations in position 43 (Oxford-3, San Dimas) and 46 (Cambridge) prevents cleavage of the propeptide, mutation in position 93 (Alabama) probably fails to bind to cell membranes, mutation in position 191 (Chapel-Hill) or in position 226 (Nagoya OR Hilo) prevent cleavage of the activation peptide. Defects in F9 are the cause of thrombophilia due to factor IX defect (THPH8). A hemostatic disorder characterized by a tendency to thrombosis. Belongs to the peptidase S1 family.

Protein type: EC 3.4.21.22; Secreted; Protease; Secreted, signal peptide

Chromosomal Location of Human Ortholog: Xq27.1-q27.2

Cellular Component: endoplasmic reticulum lumen; Golgi lumen; plasma membrane; extracellular region

Molecular Function: serine-type endopeptidase activity; calcium ion binding

Biological Process: blood coagulation, extrinsic pathway; cellular protein metabolic process; post-translational protein modification; proteolysis; blood coagulation; blood coagulation, intrinsic pathway; peptidyl-glutamic acid carboxylation

Disease: Hemophilia B; Thrombophilia, X-linked, Due To Factor Ix Defect; Coumarin Resistance
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 Research Articles on F9    1. 87 unique mutations (9 novel) were found in 225 American hemophilia B patients. c.316G>A, c.1025C>T, and c.1328T>A accounted 37.1%. Only those with large deletions and nonsense mutations had inhibitors.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with F9 elisa kitPathways associated with F9 elisa kit
 Reference Product  PubMed Publications
 F10 elisa kit  >24 publications with F9 and F10
 F12 elisa kit  >18 publications with F9 and F12
 SERPINC1 elisa kit  >3 publications with F9 and SERPINC1
 GGCX elisa kit  >1 publications with F9 and GGCX
 Products by Pathway  Pathway Diagram
 Blood Clotting Cascade Pathway antibodies  Blood Clotting Cascade Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Extrinsic Pathway antibodies  Extrinsic Pathway Diagram
 Formation Of Fibrin Clot (Clotting Cascade) Pathway antibodies  Formation Of Fibrin Clot (Clotting Cascade) Pathway Diagram
 Gamma-carboxylation Of Protein Precursors Pathway antibodies  Gamma-carboxylation Of Protein Precursors Pathway Diagram
 Gamma-carboxylation, Transport, And Amino-terminal Cleavage Of Proteins Pathway antibodies  Gamma-carboxylation, Transport, And Amino-terminal Cleavage Of Proteins Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 Intrinsic Pathway antibodies  Intrinsic Pathway Diagram
Diseases associated with F9 elisa kitOrgans/Tissues associated with F9 elisa kit
 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >868 publications with F9 and Carcinoma
 Hemophilia B Antibodies  >80 publications with F9 and Hemophilia B
 Cell Transformation, Neoplastic Antibodies  >75 publications with F9 and Cell Transformation, Neoplastic
 Immune System Diseases Antibodies  >39 publications with F9 and Immune System Diseases
 Liver Diseases Antibodies  >27 publications with F9 and Liver Diseases
 Liver Neoplasms Antibodies  >23 publications with F9 and Liver Neoplasms
 Adenocarcinoma Antibodies  >23 publications with F9 and Adenocarcinoma
 Mental Disorders Antibodies  >22 publications with F9 and Mental Disorders
 Hemophilia A Antibodies  >19 publications with F9 and Hemophilia A
 Vascular Diseases Antibodies  >17 publications with F9 and Vascular Diseases
 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >99 publications with F9 and Liver
 Uncharacterized Tissue Antibodies  >1 publications with F9 and Uncharacterized Tissue
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