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FV elisa kit :: Mouse Coagulation Factor V ELISA Kit

Scan QR to view Datasheet Catalog #    MBS760688
Typical Standard Curve/Testing Data
Unit / Price
48-Strip-Wells  /  $295 +1 FREE 8GB USB
96-Strip-Wells  /  $390 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,615 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,015 +3 FREE 8GB USB
15x96-Strip-Wells  /  $4,285 +5 FREE 8GB USB
25x96-Strip-Wells  /  $6,715 +8 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Coagulation Factor V (FV), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Coagulation Factor V ELISA Kit

 Product Synonym Names    FV/F5/Plasma Factor V)/Activated protein C cofactor/PCCF/Proaccelerin; labile factor
 Product Gene Name   

FV elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS760688 COA    COA PDF
 OMIM    188055
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 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of FV. No significant crossreactivity or interference between FV and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates and other biological fluids
 Assay Type    Quantitative Sandwich
 Detection Range    3.125-200ng/ml
 Sensitivity    <1.875ng/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level FV were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
 Inter-assay Precision    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level FV were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
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 Preparation and Storage    Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of FV elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for FV purchase    MBS760688 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Coagulation Factor V (FV) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 3.125-200ng/ml in biological research samples containing FV, with an estimated sensitivity of <1.875ng/ml. The ELISA analytical biochemical technique of the MBS760688 kit is based on FV antibody-FV antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect FV antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, FV. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for FV elisa kit

   Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- FV antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- FV antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the FV amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of FV can be calculated.
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 Typical Standard Curve/Testing Data of FV elisa kit    FV elisa kit Typical Standard Curve/Testing Data image
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Sample Manual Insert of MBS760688. Click to request current manual
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NCBI/Uniprot data below describe general gene information for FV. It may not necessarily be applicable to this product.
 NCBI GI #    105990535
 NCBI GeneID    2153
 NCBI Accession #    NP_000121.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_000130.4 [Other Products]
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 UniProt Secondary Accession #    Q14285; Q2EHR5; Q5R346; Q5R347; Q6UPU6; Q8WWQ6; A8K6E8 [Other Products]
 UniProt Related Accession #    P12259 [Other Products]
 Molecular Weight    251,703 Da
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 NCBI Official Full Name    coagulation factor V preproprotein
 NCBI Official Synonym Full Names    coagulation factor V
 NCBI Official Symbol    F5 [Similar Products]
 NCBI Official Synonym Symbols   
FVL; PCCF; THPH2; RPRGL1
[Similar Products]
 NCBI Protein Information    coagulation factor V
 UniProt Protein Name    Coagulation factor V
 UniProt Synonym Protein Names   
Activated protein C cofactor; Proaccelerin, labile factor
 Protein Family    Coagulation factor
 UniProt Gene Name    F5 [Similar Products]
 UniProt Entry Name    FA5_HUMAN
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 NCBI Summary for FV    This gene encodes an essential cofactor of the blood coagulation cascade. This factor circulates in plasma, and is converted to the active form by the release of the activation peptide by thrombin during coagulation. This generates a heavy chain and a light chain which are held together by calcium ions. The activated protein is a cofactor that participates with activated coagulation factor X to activate prothrombin to thrombin. Defects in this gene result in either an autosomal recessive hemorrhagic diathesis or an autosomal dominant form of thrombophilia, which is known as activated protein C resistance. [provided by RefSeq, Oct 2008]
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 UniProt Comments for FV    factor V: Central regulator of hemostasis. It serves as a critical cofactor for the prothrombinase activity of factor Xa that results in the activation of prothrombin to thrombin. Defects in F5 are the cause of factor V deficiency (FA5D); also known as Owren parahemophilia. It is an hemorrhagic diastesis. Defects in F5 are the cause of thrombophilia due to activated protein C resistance (THPH2). THPH2 is a hemostatic disorder due to defective degradation of factor Va by activated protein C. It is characterized by a poor anticoagulant response to activated protein C resulting in tendency to thrombosis. Defects in F5 are a cause of susceptibility to Budd- Chiari syndrome (BDCHS). A syndrome caused by obstruction of hepatic venous outflow involving either the hepatic veins or the terminal segment of the inferior vena cava. Obstructions are generally caused by thrombosis and lead to hepatic congestion and ischemic necrosis. Clinical manifestations observed in the majority of patients include hepatomegaly, right upper quadrant pain and abdominal ascites. Budd-Chiari syndrome is associated with a combination of disease states including primary myeloproliferative syndromes and thrombophilia due to factor V Leiden, protein C deficiency and antithrombin III deficiency. Budd-Chiari syndrome is a rare but typical complication in patients with polycythemia vera. Defects in F5 may be a cause of susceptibility to ischemic stroke (ISCHSTR); also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Defects in F5 are associated with susceptibility to pregnancy loss, recurrent, type 1 (RPRGL1). RPRGL1 is a common complication of pregnancy, resulting in spontaneous abortion before the fetus has reached viability. The term includes all miscarriages from the time of conception until 24 weeks of gestation. Recurrent pregnancy loss is defined as 3 or more consecutive spontaneous abortions. Belongs to the multicopper oxidase family.

Protein type: Secreted; Protease; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1q23

Cellular Component: endoplasmic reticulum lumen; ER to Golgi transport vesicle; ER-Golgi intermediate compartment membrane; extracellular region; extracellular space; Golgi membrane; membrane; plasma membrane

Molecular Function: copper ion binding; protein binding

Biological Process: blood circulation; blood coagulation; cellular protein metabolic process; COPII coating of Golgi vesicle; ER to Golgi vesicle-mediated transport; platelet activation; platelet degranulation; post-translational protein modification; protein amino acid N-linked glycosylation via asparagine

Disease: Budd-chiari Syndrome; Factor V Deficiency; Pregnancy Loss, Recurrent, Susceptibility To, 1; Stroke, Ischemic; Thrombophilia Due To Activated Protein C Resistance
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 Research Articles on FV    1. Our study does not support the notion that factor V HR2 haplotype might be a risk factor for thrombosis despite its high prevalence among patients with PE.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with FV elisa kitPathways associated with FV elisa kit
 Reference Product  PubMed Publications
 F10 elisa kit  >42 publications with FV and F10
 PROC elisa kit  >23 publications with FV and PROC
 SERPINE1 elisa kit  >12 publications with FV and SERPINE1
 SERPINC1 elisa kit  >9 publications with FV and SERPINC1
 VWF elisa kit  >8 publications with FV and VWF
 PROS1 elisa kit  >4 publications with FV and PROS1
 PLG elisa kit  >1 publications with FV and PLG
 Products by Pathway  Pathway Diagram
 Asparagine N-linked Glycosylation Pathway antibodies  Asparagine N-linked Glycosylation Pathway Diagram
 Blood Clotting Cascade Pathway antibodies  Blood Clotting Cascade Pathway Diagram
 COPII (Coat Protein 2) Mediated Vesicle Transport Pathway antibodies  COPII (Coat Protein 2) Mediated Vesicle Transport Pathway Diagram
 Cargo Concentration In The ER Pathway antibodies  Cargo Concentration In The ER Pathway Diagram
 Common Pathway Of Fibrin Clot Formation antibodies  Common Pathway Of Fibrin Clot Formation Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 ER To Golgi Anterograde Transport Pathway antibodies  ER To Golgi Anterograde Transport Pathway Diagram
 Formation Of Fibrin Clot (Clotting Cascade) Pathway antibodies  Formation Of Fibrin Clot (Clotting Cascade) Pathway Diagram
Diseases associated with FV elisa kitOrgans/Tissues associated with FV elisa kit
 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >115 publications with FV and Cardiovascular Diseases
 Thrombosis Antibodies  >71 publications with FV and Thrombosis
 Immune System Diseases Antibodies  >51 publications with FV and Immune System Diseases
 Blood Coagulation Disorders Antibodies  >40 publications with FV and Blood Coagulation Disorders
 Inflammation Antibodies  >34 publications with FV and Inflammation
 Venous Thrombosis Antibodies  >33 publications with FV and Venous Thrombosis
 Thromboembolism Antibodies  >31 publications with FV and Thromboembolism
 Venous Thromboembolism Antibodies  >28 publications with FV and Venous Thromboembolism
 Liver Diseases Antibodies  >25 publications with FV and Liver Diseases
 Factor V Deficiency Antibodies  >23 publications with FV and Factor V Deficiency
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >386 publications with FV and Blood
 Brain Antibodies  >213 publications with FV and Brain
 Liver Antibodies  >65 publications with FV and Liver
 Thymus Antibodies  >43 publications with FV and Thymus
 Heart Antibodies  >41 publications with FV and Heart
 Intestine Antibodies  >37 publications with FV and Intestine
 Lung Antibodies  >37 publications with FV and Lung
 Kidney Antibodies  >34 publications with FV and Kidney
 Eye Antibodies  >31 publications with FV and Eye
 Embryonic Tissue Antibodies  >22 publications with FV and Embryonic Tissue
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