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COL1a1 elisa kit :: Mouse Collagen Type I Alpha 1 (COL1a1) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS453081 (SPECIAL PROMOTIONAL PRICING for a limited time)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $350 +1 FREE 8GB USB
96-Strip-Wells  /  $465 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,070 +2 FREE 8GB USB
10x96-Strip-Wells  /  $4,010 +5 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Collagen Type I Alpha 1 (COL1a1), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Collagen Type I Alpha 1 (COL1a1) ELISA Kit

 Product Synonym Names    COL-1A1; COL1-A1; COL1A-1; OI4; Collagen Alpha-1(I)chain
 Product Gene Name   

COL1a1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 3D Structure    ModBase 3D Structure for P11087
 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of COL1A1. No significant cross-reactivity or interference between COL1A1 and analogues was observed.
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 Samples    Serum, plasma and other biological fluids.
 Assay Type    Sandwich
 Detection Range    0.312-20ng/mL
 Sensitivity    0.137ng/mL
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level COL1A1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
 Inter-assay Precision    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level COL1A1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
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 Preparation and Storage    For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of COL1a1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for COL1a1 purchase    MBS453081 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Collagen Type I Alpha 1 (COL1a1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.312-20ng/mL in biological research samples containing COL1a1, with an estimated sensitivity of 0.137ng/mL. The ELISA analytical biochemical technique of the MBS453081 kit is based on COL1a1 antibody-COL1a1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect COL1a1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, COL1a1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for COL1a1 elisa kit

   Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of COL1A1 in mouse serum, plasma and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to COL1A1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to COL1A1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain COL1A1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of COL1A1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of COL1a1 elisa kit    COL1a1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS453081. Click to request current manual
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NCBI/Uniprot data below describe general gene information for COL1a1. It may not necessarily be applicable to this product.
 NCBI GI #    34328108
 NCBI GeneID    12842
 NCBI Accession #    NP_031768.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_007742.4 [Other Products]
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 UniProt Primary Accession #    P11087 [Other Products]
 UniProt Secondary Accession #    Q53WT0; Q60635; Q61367; Q61427; Q63919; Q6PCL3; Q810J9 [Other Products]
 UniProt Related Accession #    P11087 [Other Products]
 Molecular Weight    117,820 Da
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 NCBI Official Full Name    collagen alpha-1(I) chain preproprotein
 NCBI Official Synonym Full Names    collagen, type I, alpha 1
 NCBI Official Symbol    Col1a1 [Similar Products]
 NCBI Official Synonym Symbols   
Cola1; Mov13; Cola-1; Mov-13; Col1a-1
[Similar Products]
 NCBI Protein Information    collagen alpha-1(I) chain
 UniProt Protein Name    Collagen alpha-1(I) chain
 UniProt Synonym Protein Names   
Alpha-1 type I collagen
 Protein Family    Collagen
 UniProt Gene Name    Col1a1 [Similar Products]
 UniProt Synonym Gene Names    Cola1 [Similar Products]
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 NCBI Summary for COL1a1    This gene encodes the alpha-1 subunit of the fibril-forming type I collagen, the most abundant protein of bone, skin and tendon extracellular matrices. The encoded protein, in association with alpha-2 subunit, forms heterotrimeric type I procollagen that undergoes proteolytic processing during fibril formation. Mice lacking the encoded protein die in utero caused by the rupture of a major blood vessel. Transgenic mice expressing significantly lower levels of this gene exhibit morphological and functional defects in mineralized and non-mineralized connective tissue and, progressive loss of hearing. [provided by RefSeq, Nov 2015]
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 UniProt Comments for COL1a1    Type I collagen is a member of group I collagen (fibrillar forming collagen).
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 Research Articles on COL1a1    1. the rate of collagen I degradation was increased in Poldip2(+/-) vs. Poldip2(+/+) MASMs. Conversely, activation of the PI3K/Akt/mTOR signaling pathway, involved in regulation of protein synthesis, was significantly elevated in Poldip2(+/-) MASMs as was beta1-integrin expression.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with COL1a1 elisa kitPathways associated with COL1a1 elisa kit
 Reference Product  PubMed Publications
 Col1a2 elisa kit  >409 publications with COL1a1 and Col1a2
 Col3a1 elisa kit  >252 publications with COL1a1 and Col3a1
 Col5a1 elisa kit  >60 publications with COL1a1 and Col5a1
 Col5a2 elisa kit  >37 publications with COL1a1 and Col5a2
 Col4a1 elisa kit  >35 publications with COL1a1 and Col4a1
 Col6a3 elisa kit  >15 publications with COL1a1 and Col6a3
 Col6a1 elisa kit  >14 publications with COL1a1 and Col6a1
 Col6a2 elisa kit  >4 publications with COL1a1 and Col6a2
 Col8a1 elisa kit  >2 publications with COL1a1 and Col8a1
 Products by Pathway  Pathway Diagram
 AGE-RAGE Signaling Pathway In Diabetic Complications antibodies  AGE-RAGE Signaling Pathway In Diabetic Complications Diagram
 AGE-RAGE Signaling Pathway In Diabetic Complications antibodies  AGE-RAGE Signaling Pathway In Diabetic Complications Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway antibodies  Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway Diagram
 Collagen Biosynthesis And Modifying Enzymes Pathway antibodies  Collagen Biosynthesis And Modifying Enzymes Pathway Diagram
 Collagen Chain Trimerization Pathway antibodies  Collagen Chain Trimerization Pathway Diagram
 Collagen Formation Pathway antibodies  Collagen Formation Pathway Diagram
 Dysregulated MiRNA Targeting In Insulin/PI3K-AKT Signaling Pathway antibodies  Dysregulated MiRNA Targeting In Insulin/PI3K-AKT Signaling Pathway Diagram
 ECM-receptor Interaction Pathway antibodies  ECM-receptor Interaction Pathway Diagram
Diseases associated with COL1a1 elisa kitOrgans/Tissues associated with COL1a1 elisa kit
 Disease Name  Pubmed Publications
 Osteogenesis Imperfecta Antibodies  >351 publications with COL1a1 and Osteogenesis Imperfecta
 Fibrosis Antibodies  >320 publications with COL1a1 and Fibrosis
 Osteoporosis Antibodies  >150 publications with COL1a1 and Osteoporosis
 Disease Models, Animal Antibodies  >135 publications with COL1a1 and Disease Models, Animal
 Inflammation Antibodies  >130 publications with COL1a1 and Inflammation
 Liver Diseases Antibodies  >88 publications with COL1a1 and Liver Diseases
 Liver Cirrhosis Antibodies  >64 publications with COL1a1 and Liver Cirrhosis
 Necrosis Antibodies  >55 publications with COL1a1 and Necrosis
 Heart Diseases Antibodies  >46 publications with COL1a1 and Heart Diseases
 Kidney Diseases Antibodies  >33 publications with COL1a1 and Kidney Diseases
 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >767 publications with COL1a1 and Bone
 Connective Tissue Antibodies  >355 publications with COL1a1 and Connective Tissue
 Skin Antibodies  >280 publications with COL1a1 and Skin
 Muscle Antibodies  >215 publications with COL1a1 and Muscle
 Joint Antibodies  >147 publications with COL1a1 and Joint
 Liver Antibodies  >140 publications with COL1a1 and Liver
 Bone Marrow Antibodies  >124 publications with COL1a1 and Bone Marrow
 Lung Antibodies  >86 publications with COL1a1 and Lung
 Heart Antibodies  >67 publications with COL1a1 and Heart
 Kidney Antibodies  >59 publications with COL1a1 and Kidney
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