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C5 elisa kit :: Mouse Complement C5 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS763175
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $280 +1 FREE 8GB USB
96-Strip-Wells  /  $375 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,615 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,015 +3 FREE 8GB USB
15x96-Strip-Wells  /  $4,285 +5 FREE 8GB USB
25x96-Strip-Wells  /  $6,715 +8 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Complement C5, ELISA Kit

★Popular Item★
 Also Known As   

Mouse Complement C5 ELISA Kit

 Product Synonym Names    C5
 Product Gene Name   

C5 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS763175 COA    COA PDF
 OMIM    120900
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 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of C5. No significant crossreactivity or interference between C5 and analogues was observed.
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 Samples    Serum, plasma and other biological fluids.
 Assay Type    Sandwich ELISA, Double Antibody
 Detection Range    0.156-10ng/ml
 Sensitivity    0.094ng/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level C5 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
 Inter-assay Precision    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level C5 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
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 Preparation and Storage    Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of C5 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for C5 purchase    MBS763175 is a ready-to-use microwell, strip plate Sandwich, Double Antibody ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Complement C5, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.156-10ng/ml in biological research samples containing C5, with an estimated sensitivity of 0.094ng/ml. The ELISA analytical biochemical technique of the MBS763175 kit is based on C5 antibody-C5 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect C5 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, C5. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for C5 elisa kit

   Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- C5 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- C5 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the C5 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of C5 can be calculated.
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 Typical Testing Data/Standard Curve (for reference only) of C5 elisa kit    C5 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS763175. Click to request current manual
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NCBI/Uniprot data below describe general gene information for C5. It may not necessarily be applicable to this product.
 NCBI GI #    38016947
 NCBI GeneID    727
 NCBI Accession #    NP_001726.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_001735.2 [Other Products]
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 UniProt Secondary Accession #    Q14CJ0; Q27I61 [Other Products]
 UniProt Related Accession #    P01031 [Other Products]
 Molecular Weight    188,305 Da
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 NCBI Official Full Name    complement C5 isoform 1 preproprotein
 NCBI Official Synonym Full Names    complement component 5
 NCBI Official Symbol    C5 [Similar Products]
 NCBI Official Synonym Symbols   
C5D; C5a; C5b; ECLZB; CPAMD4
[Similar Products]
 NCBI Protein Information    complement C5
 UniProt Protein Name    Complement C5
 UniProt Synonym Protein Names   
C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4
 Protein Family    Complement C5
 UniProt Gene Name    C5 [Similar Products]
 UniProt Synonym Gene Names    CPAMD4 [Similar Products]
 UniProt Entry Name    CO5_HUMAN
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 NCBI Summary for C5    This gene encodes a component of the complement system, a part of the innate immune system that plays an important role in inflammation, host homeostasis, and host defense against pathogens. The encoded preproprotein is proteolytically processed to generate multiple protein products, including the C5 alpha chain, C5 beta chain, C5a anaphylatoxin and C5b. The C5 protein is comprised of the C5 alpha and beta chains, which are linked by a disulfide bridge. Cleavage of the alpha chain by a convertase enzyme results in the formation of the C5a anaphylatoxin, which possesses potent spasmogenic and chemotactic activity, and the C5b macromolecular cleavage product, a subunit of the membrane attack complex (MAC). Mutations in this gene cause complement component 5 deficiency, a disease characterized by recurrent bacterial infections. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2015]
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 UniProt Comments for C5    C5: Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled. Defects in C5 are the cause of complement component 5 deficiency (C5D). A rare defect of the complement classical pathway associated with susceptibility to severe recurrent infections, predominantly by Neisseria gonorrhoeae or Neisseria meningitidis. An association study of C5 haplotypes and genotypes in individuals with chronic hepatitis C virus infection shows that individuals homozygous for the C5_1 haplotype have a significantly higher stage of liver fibrosis than individuals carrying at least 1 other allele (PubMed:15995705).

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 9q33-q34

Cellular Component: extracellular region; extracellular space; membrane attack complex

Molecular Function: chemokine activity; endopeptidase inhibitor activity; protein binding; receptor binding

Biological Process: activation of MAPK activity; cell surface receptor linked signal transduction; chemotaxis; complement activation; complement activation, alternative pathway; complement activation, classical pathway; cytolysis; G-protein coupled receptor protein signaling pathway; in utero embryonic development; inflammatory response; innate immune response; positive regulation of angiogenesis; regulation of complement activation; response to stress

Disease: Complement Component 5 Deficiency; Eculizumab, Poor Response To
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 Research Articles on C5    1. Complement C5b-9 complex sensitizes 661W photoreceptor cells to both apoptosis and necroptosis.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with C5 elisa kitPathways associated with C5 elisa kit
 Reference Product  PubMed Publications
 C5AR1 elisa kit  >18 publications with C5 and C5AR1
 C8A elisa kit  >5 publications with C5 and C8A
 GPR77 elisa kit  >5 publications with C5 and GPR77
 C8B elisa kit  >3 publications with C5 and C8B
 C3AR1 elisa kit  >2 publications with C5 and C3AR1
 CXCR7 elisa kit  >1 publications with C5 and CXCR7
 BDKRB2 elisa kit  >1 publications with C5 and BDKRB2
 Products by Pathway  Pathway Diagram
 Activation Of C3 And C5 Pathway antibodies  Activation Of C3 And C5 Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 Complement Activation, Classical Pathway antibodies  Complement Activation, Classical Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement Cascade Pathway antibodies  Complement Cascade Pathway Diagram
 G Alpha (i) Signalling Events Pathway antibodies  G Alpha (i) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 Herpes Simplex Infection Pathway antibodies  Herpes Simplex Infection Pathway Diagram
Diseases associated with C5 elisa kitOrgans/Tissues associated with C5 elisa kit
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >2391 publications with C5 and Nervous System Diseases
 Neurologic Manifestations Antibodies  >1010 publications with C5 and Neurologic Manifestations
 Inflammation Antibodies  >753 publications with C5 and Inflammation
 Cardiovascular Diseases Antibodies  >695 publications with C5 and Cardiovascular Diseases
 Vascular Diseases Antibodies  >672 publications with C5 and Vascular Diseases
 Kidney Diseases Antibodies  >465 publications with C5 and Kidney Diseases
 Anemia Antibodies  >276 publications with C5 and Anemia
 Immunologic Deficiency Syndromes Antibodies  >264 publications with C5 and Immunologic Deficiency Syndromes
 Necrosis Antibodies  >233 publications with C5 and Necrosis
 Heart Diseases Antibodies  >177 publications with C5 and Heart Diseases
 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >2298 publications with C5 and Bone
 Muscle Antibodies  >1284 publications with C5 and Muscle
 Brain Antibodies  >785 publications with C5 and Brain
 Kidney Antibodies  >511 publications with C5 and Kidney
 Lung Antibodies  >484 publications with C5 and Lung
 Liver Antibodies  >466 publications with C5 and Liver
 Spleen Antibodies  >172 publications with C5 and Spleen
 Bone Marrow Antibodies  >135 publications with C5 and Bone Marrow
 Intestine Antibodies  >123 publications with C5 and Intestine
 Eye Antibodies  >116 publications with C5 and Eye
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