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CFI elisa kit :: Mouse Complement Factor I ELISA Kit

Scan QR to view Datasheet Catalog #    MBS726178
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $440 +1 FREE 8GB USB
96-Strip-Wells  /  $640 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Complement Factor I (CFI), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Complement Factor I ELISA Kit

 Product Gene Name   

CFI elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    615439
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 Species Reactivity    Mouse
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Sandwich
 Detection Range    2.5-50ng/mL
 Sensitivity    0.1ng/mL
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of CFI elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for CFI purchase    MBS726178 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Complement Factor I (CFI) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 2.5-50ng/mL in biological research samples containing CFI, with an estimated sensitivity of 0.1ng/mL. The ELISA analytical biochemical technique of the MBS726178 kit is based on CFI antibody-CFI antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CFI antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CFI. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for CFI elisa kit

   Intended Uses: This CFI ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse CFI. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: CFI ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for CFI. Standards or samples are then added to the microtiter plate wells and CFI if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of CFI present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for CFI are added to each well to "sandwich" the CFI immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain CFI and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CFI concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for CFI elisa kit    Immunology
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 Typical Testing Data/Standard Curve (for reference only) of CFI elisa kit    CFI elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS726178. Click to request current manual
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NCBI/Uniprot data below describe general gene information for CFI. It may not necessarily be applicable to this product.
 NCBI GI #    119392081
 NCBI GeneID    3426
 NCBI Accession #    NP_000195.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_000204.3 [Other Products]
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 UniProt Secondary Accession #    O60442 [Other Products]
 UniProt Related Accession #    P05156 [Other Products]
 Molecular Weight    65,750 Da
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 NCBI Official Full Name    complement factor I preproprotein
 NCBI Official Synonym Full Names    complement factor I
 NCBI Official Symbol    CFI [Similar Products]
 NCBI Official Synonym Symbols   
FI; IF; KAF; AHUS3; ARMD13; C3BINA; C3b-INA
[Similar Products]
 NCBI Protein Information    complement factor I; C3b-inactivator; C3B/C4B inactivator; complement component I; light chain of factor I; Konglutinogen-activating factor; complement factor I heavy chain; complement control protein factor I
 UniProt Protein Name    Complement factor I
 UniProt Synonym Protein Names   
C3B/C4B inactivatorCleaved into the following 2 chains:Complement factor I heavy chain; Complement factor I light chain
 Protein Family    Complement factor
 UniProt Gene Name    CFI [Similar Products]
 UniProt Synonym Gene Names    IF [Similar Products]
 UniProt Entry Name    CFAI_HUMAN
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 NCBI Summary for CFI    This gene encodes a serine proteinase that is essential for regulating the complement cascade. The encoded preproprotein is cleaved to produce both heavy and light chains, which are linked by disulfide bonds to form a heterodimeric glycoprotein. This heterodimer can cleave and inactivate the complement components C4b and C3b, and it prevents the assembly of the C3 and C5 convertase enzymes. Defects in this gene cause complement factor I deficiency, an autosomal recessive disease associated with a susceptibility to pyogenic infections. Mutations in this gene have been associated with a predisposition to atypical hemolytic uraemic syndrome, a disease characterized by acute renal failure, microangiopathic hemolytic anemia and thrombocytopenia. Primary glomerulonephritis with immmune deposits is another condition associated with mutation of this gene. [provided by RefSeq, Jul 2008]
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 UniProt Comments for CFI    CFI: Responsible for cleaving the alpha-chains of C4b and C3b in the presence of the cofactors C4-binding protein and factor H respectively. Defects in CFI are a cause of susceptibility to hemolytic uremic syndrome atypical type 3 (AHUS3). An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype. Defects in CFI are the cause of complement factor I deficiency (CFI deficiency). CFI deficiency is an autosomal recessive condition associated with a propensity to pyogenic infections. Belongs to the peptidase S1 family.

Protein type: Secreted; Protease; EC 3.4.21.45; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 4q25

Cellular Component: extracellular space; membrane; extracellular region; nucleus

Molecular Function: metal ion binding; serine-type endopeptidase activity; scavenger receptor activity

Biological Process: receptor-mediated endocytosis; regulation of complement activation; innate immune response; proteolysis; complement activation, classical pathway

Disease: Macular Degeneration, Age-related, 13; Complement Factor I Deficiency; Hemolytic Uremic Syndrome, Atypical, Susceptibility To, 3
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 Research Articles on CFI    1. Recurrent aseptic meningo-encephalitis is a rare clinical presentation of complete FI deficiency.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with CFI elisa kitPathways associated with CFI elisa kit
 Reference Product  PubMed Publications
 CFH elisa kit  >60 publications with CFI and CFH
 CD46 elisa kit  >31 publications with CFI and CD46
 SERPINC1 elisa kit  >3 publications with CFI and SERPINC1
 CR1 elisa kit  >2 publications with CFI and CR1
 C4BPA elisa kit  >2 publications with CFI and C4BPA
 Products by Pathway  Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement Cascade Pathway antibodies  Complement Cascade Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Innate Immune System Pathway antibodies  Innate Immune System Pathway Diagram
 Regulation Of Complement Cascade Pathway antibodies  Regulation Of Complement Cascade Pathway Diagram
 Staphylococcus Aureus Infection Pathway antibodies  Staphylococcus Aureus Infection Pathway Diagram
 Staphylococcus Aureus Infection Pathway antibodies  Staphylococcus Aureus Infection Pathway Diagram
Diseases associated with CFI elisa kitOrgans/Tissues associated with CFI elisa kit
 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >113 publications with CFI and Cardiovascular Diseases
 Heart Diseases Antibodies  >84 publications with CFI and Heart Diseases
 Kidney Diseases Antibodies  >57 publications with CFI and Kidney Diseases
 Anemia Antibodies  >48 publications with CFI and Anemia
 Anemia, Hemolytic Antibodies  >43 publications with CFI and Anemia, Hemolytic
 Brain Diseases Antibodies  >41 publications with CFI and Brain Diseases
 Inflammation Antibodies  >34 publications with CFI and Inflammation
 Atypical hemolytic uremic syndrome Antibodies  >31 publications with CFI and Atypical hemolytic uremic syndrome
 Macular Degeneration Antibodies  >22 publications with CFI and Macular Degeneration
 Liver Diseases Antibodies  >21 publications with CFI and Liver Diseases
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >214 publications with CFI and Blood
 Heart Antibodies  >69 publications with CFI and Heart
 Vascular Antibodies  >66 publications with CFI and Vascular
 Brain Antibodies  >47 publications with CFI and Brain
 Kidney Antibodies  >41 publications with CFI and Kidney
 Lung Antibodies  >33 publications with CFI and Lung
 Liver Antibodies  >25 publications with CFI and Liver
 Eye Antibodies  >23 publications with CFI and Eye
 Skin Antibodies  >19 publications with CFI and Skin
 Muscle Antibodies  >13 publications with CFI and Muscle
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