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C8A elisa kit :: Mouse Complement component C8 alpha chain (C8A) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS7228843
Typical Testing Data/Standard Curve
Unit / Price
48-Strip-Wells  /  $440 +1 FREE 8GB USB
96-Strip-Wells  /  $640 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
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 Product Name   

Complement component C8 alpha chain (C8A), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Complement component C8 alpha chain (C8A) ELISA Kit

 Product Gene Name   

C8A elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    gene 613790
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 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of C8alpha. No significant cross-reactivity or interference between C8alpha and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between C8alpha and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Sensitivity    0.1 ng/mL.
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of C8A elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for C8A purchase    MBS7228843 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Complement component C8 alpha chain (C8A) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing C8A, with an estimated sensitivity of 0.1 ng/mL. The ELISA analytical biochemical technique of the MBS7228843 kit is based on C8A antibody-C8A antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect C8A antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, C8A. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for C8A elisa kit

   Intended Uses: This C8alpha ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse C8alpha. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay||C8alpha ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-C8alpha antibody and an C8alpha-HRP conjugate. The assay sample and buffer are incubated together with C8alpha-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the C8alpha concentration since C8alpha from samples and C8alpha-HRP conjugate compete for the anti-C8alpha antibody binding site. Since the number of sites is limited, as more sites are occupied by C8alpha from the sample, fewer sites are left to bind C8alpha-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The C8alpha concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for C8A elisa kit    Immunology
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 Typical Testing Data/Standard Curve of C8A elisa kit    C8A elisa kit Typical Testing Data/Standard Curve image
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Sample Manual Insert of MBS7228843. Click to request current manual
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NCBI/Uniprot data below describe general gene information for C8A. It may not necessarily be applicable to this product.
 NCBI GI #    4557389
 NCBI GeneID    731
 NCBI Accession #    NP_000553.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_000562.2 [Other Products]
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 UniProt Secondary Accession #    Q13668; Q9H130; A2RUI4; A2RUI5 [Other Products]
 UniProt Related Accession #    P07357 [Other Products]
 Molecular Weight    65,163 Da
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 NCBI Official Full Name    complement component C8 alpha chain
 NCBI Official Synonym Full Names    complement component 8, alpha polypeptide
 NCBI Official Symbol    C8A [Similar Products]
 NCBI Protein Information    complement component C8 alpha chain; complement component 8 subunit alpha
 UniProt Protein Name    Complement component C8 alpha chain
 UniProt Synonym Protein Names   
Complement component 8 subunit alpha
 Protein Family    Complement component
 UniProt Gene Name    C8A [Similar Products]
 UniProt Entry Name    CO8A_HUMAN
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 NCBI Summary for C8A    C8 is a component of the complement system and contains three polypeptides, alpha, beta and gamma. This gene encodes the alpha subunit of C8. C8 participates in the formation of the membrane attack complex (MAC). The MAC assembles on bacterial membranes to form a pore, permitting disruption of bacterial membrane organization. Mutations in this gene cause complement C8 alpha-gamma deficiency. [provided by RefSeq, Nov 2008]
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 UniProt Comments for C8A    C8A: Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. C8A inserts into the target membrane, but does not form pores by itself. Defects in C8A are a cause of complement component 8 deficiency type 1 (C8D1). A rare defect of the complement classical pathway associated with susceptibility to severe recurrent infections, predominantly by Neisseria gonorrhoeae or Neisseria meningitidis. Belongs to the complement C6/C7/C8/C9 family.

Protein type: Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 1p32

Cellular Component: membrane attack complex; extracellular space; membrane; extracellular region

Molecular Function: complement binding; protein complex binding

Biological Process: cytolysis; complement activation, alternative pathway; regulation of complement activation; innate immune response; immune response; complement activation, classical pathway; complement activation

Disease: Complement Component 8 Deficiency, Type I
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 Research Articles on C8A    1. C8 alpha and C8 beta have correspondingly similar roles in MAC-mediated lysis of erythrocytes and bacterial killing. C8 gamma is not required for complement-mediated killing of Gram-negative bacteria.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with C8A elisa kitPathways associated with C8A elisa kit
 Reference Product  PubMed Publications
 C8B elisa kit  >17 publications with C8A and C8B
 C8G elisa kit  >8 publications with C8A and C8G
 CD59 elisa kit  >1 publications with C8A and CD59
 Products by Pathway  Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Complement Activation, Classical Pathway antibodies  Complement Activation, Classical Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement Cascade Pathway antibodies  Complement Cascade Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Innate Immune System Pathway antibodies  Innate Immune System Pathway Diagram
 Prion Diseases Pathway antibodies  Prion Diseases Pathway Diagram
 Prion Diseases Pathway antibodies  Prion Diseases Pathway Diagram
Diseases associated with C8A elisa kitOrgans/Tissues associated with C8A elisa kit
 Disease Name  Pubmed Publications
 Meningitis Antibodies  >3 publications with C8A and Meningitis
 Nervous System Diseases Antibodies  >2 publications with C8A and Nervous System Diseases
 Brain Diseases Antibodies  >2 publications with C8A and Brain Diseases
 Inflammation Antibodies  >2 publications with C8A and Inflammation
 Neisseriaceae Infections Antibodies  >2 publications with C8A and Neisseriaceae Infections
 Mental Disorders Antibodies  >1 publications with C8A and Mental Disorders
 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >4 publications with C8A and Liver
 Blood Antibodies  >3 publications with C8A and Blood
 Lung Antibodies  >1 publications with C8A and Lung
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