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Cfb elisa kit :: Mouse Complement factor B ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2882240
Typical Standard Curve/Testing Data
Unit / Price
48-Strip-Wells  /  $380 +1 FREE 8GB USB
96-Strip-Wells  /  $500 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,170 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,840 +4 FREE 8GB USB
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 Product Name   

Complement factor B (Cfb), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Complement factor B ELISA Kit

 Product Synonym Names    Complement factor B; C3/C5 convertase; Cfb; Bf; H2-Bf;
 Product Gene Name   

Cfb elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS2882240 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for P04186
 Species Reactivity    Mouse
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 Assay Type    Sandwich
 Detection Range    78-5000 pg/mL
 Sensitivity    39.8 pg/mL
 Intra-Assay CV    <=6.9%
 Inter-Assay CV    <=8.8%
 Recovery    106%
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 Preparation and Storage    For long term storage, please store the entire kit at -20 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of Cfb elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for Cfb purchase    MBS2882240 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Complement factor B (Cfb) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 78-5000 pg/mL in biological research samples containing Cfb, with an estimated sensitivity of 39.8 pg/mL. The ELISA analytical biochemical technique of the MBS2882240 kit is based on Cfb antibody-Cfb antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Cfb antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Cfb. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for Cfb elisa kit

   Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Standard Curve/Testing Data of Cfb elisa kit    Cfb elisa kit Typical Standard Curve/Testing Data image
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Sample Manual Insert of MBS2882240. Click to request current manual
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NCBI/Uniprot data below describe general gene information for Cfb. It may not necessarily be applicable to this product.
 NCBI GI #    116126
 NCBI GeneID    14962
 NCBI Accession #    P04186.2 [Other Products]
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 UniProt Primary Accession #    P04186 [Other Products]
 UniProt Related Accession #    P04186 [Other Products]
 Molecular Weight    85,004 Da
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 NCBI Official Full Name    Complement factor B
 NCBI Official Synonym Full Names    complement factor B
 NCBI Official Symbol    Cfb [Similar Products]
 NCBI Official Synonym Symbols   
B; Bf; C2; Fb; H2-Bf; AI195813; AI255840
[Similar Products]
 NCBI Protein Information    complement factor B
 UniProt Protein Name    Complement factor B
 UniProt Synonym Protein Names   
C3/C5 convertase
 Protein Family    Complement factor
 UniProt Gene Name    Cfb [Similar Products]
 UniProt Synonym Gene Names    Bf; H2-Bf [Similar Products]
 UniProt Entry Name    CFAB_MOUSE
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 UniProt Comments for Cfb    CFB: Factor B which is part of the alternate pathway of the complement system is cleaved by factor D into 2 fragments: Ba and Bb. Bb, a serine protease, then combines with complement factor 3b to generate the C3 or C5 convertase. It has also been implicated in proliferation and differentiation of preactivated B- lymphocytes, rapid spreading of peripheral blood monocytes, stimulation of lymphocyte blastogenesis and lysis of erythrocytes. Ba inhibits the proliferation of preactivated B-lymphocytes. Defects in CFB are a cause of susceptibility to hemolytic uremic syndrome atypical type 4 (AHUS4). An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype. Belongs to the peptidase S1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted; EC; Protease

Cellular Component: extracellular region; extracellular space

Molecular Function: hydrolase activity; peptidase activity; serine-type endopeptidase activity; serine-type peptidase activity

Biological Process: cell proliferation; complement activation; complement activation, alternative pathway; immune system process; innate immune response; proteolysis
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 Research Articles on Cfb    1. Study shows that Factor B regulates UVA and UVB induced immunosuppression, UV induced edema and mast cell infiltration into the skin
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with Cfb elisa kitPathways associated with Cfb elisa kit
 Reference Product  PubMed Publications
 Cfh elisa kit  >105 publications with Cfb and Cfh
 Cfi elisa kit  >37 publications with Cfb and Cfi
 C4b elisa kit  >5 publications with Cfb and C4b
 Cfd elisa kit  >5 publications with Cfb and Cfd
 Cd55 elisa kit  >3 publications with Cfb and Cd55
 Cr1l elisa kit  >1 publications with Cfb and Cr1l
 Products by Pathway  Pathway Diagram
 Activation Of C3 And C5 Pathway antibodies  Activation Of C3 And C5 Pathway Diagram
 Alternative Complement Activation Pathway antibodies  Alternative Complement Activation Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement Cascade Pathway antibodies  Complement Cascade Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Initial Triggering Of Complement Pathway antibodies  Initial Triggering Of Complement Pathway Diagram
 Innate Immune System Pathway antibodies  Innate Immune System Pathway Diagram
 Regulation Of Complement Cascade Pathway antibodies  Regulation Of Complement Cascade Pathway Diagram
Organs/Tissues associated with Cfb elisa kit
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >32 publications with Cfb and Muscle
 Liver Antibodies  >26 publications with Cfb and Liver
 Joint Antibodies  >23 publications with Cfb and Joint
 Kidney Antibodies  >18 publications with Cfb and Kidney
 Intestine Antibodies  >15 publications with Cfb and Intestine
 Brain Antibodies  >12 publications with Cfb and Brain
 Skin Antibodies  >10 publications with Cfb and Skin
 Lung Antibodies  >9 publications with Cfb and Lung
 Connective Tissue Antibodies  >4 publications with Cfb and Connective Tissue
 Bone Marrow Antibodies  >4 publications with Cfb and Bone Marrow
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