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CX43 elisa kit :: Mouse Connexin 43 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS729401
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $440 +1 FREE 8GB USB
96-Strip-Wells  /  $640 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Connexin 43 (CX43), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Connexin 43 ELISA Kit

 Product Gene Name   

CX43 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 Chromosome Location    Chromosome: 20; NC_007131.5 (40746894..40752184, complement). Location: chromosome: 20
 3D Structure    ModBase 3D Structure for O57474
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 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of CX43. No significant cross-reactivity or interference between CX43 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CX43 and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Sandwich
 Sensitivity    1.0 pg/mL.
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 Preparation and Storage    Store all reagents at 2-8 degree C
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of CX43 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for CX43 purchase    MBS729401 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Connexin 43 (CX43) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CX43, with an estimated sensitivity of 1.0 pg/mL. The ELISA analytical biochemical technique of the MBS729401 kit is based on CX43 antibody-CX43 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CX43 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CX43. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%). Intended use of this ELISA Kit is quantitative detection of murine connexin 43.

Kitís target analyte, connexin 43, is one of major integral membrane proteins that form gap junction channels to provide inter-cellular communication such as exchange of small molecules and ions between adjacent cells. Itís expressed in variable cell types and plays important role in development, differentiation, and metabolic homeostasis.

Connexin 43 is listed in NCBI and UniProt data bases as gap junction alpha-1 protein, short fin protein; gap junction protein, and Cx43.

Murine connexin 43 test system is designed as sandwich-type ELISA and concentration of connexin 43 is measured using pair of antibodies specific to target protein.

Kit is applicable for testing connexin 43 in serum, plasma, cell culture supernatants, bodily fluids, and tissue homogenates.

Product contains microwell plate pre-coated with monoclonal capture antibodies against connexin 43 and all reagents required for its quantification, including sample and reagent diluents, and wash solution.

Assay kit provides colorimetric detection of connexin 43 using polyclonal detector antibodies against connexin 43 conjugated to HRP, and TMB as its substrate solution.

Minimal detectable concentration of ELISA kit is 1pg/mL; kits are supplied with six levels of liquid calibrators between 0 and 2500pg/mL.

Intra- and inter-lot assay CV values, reported as kitís precision characteristics, are not greater than 9% and 10%, respectively.

Assay doesnít show significant cross-reactivity with analogous molecules.

ELISA kit and its reagents and components require refrigeration and must be stored at 2-8įC until indicated expiration date.
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Related Product Information for CX43 elisa kit

   Intended Uses: This CX43 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse CX43. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: CX43 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for CX43. Standards or samples are then added to the microtiter plate wells and CX43 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of CX43 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for CX43 are added to each well to "sandwich" the CX43 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain CX43 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CX43 concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for CX43 elisa kit    Cell Biology
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 Typical Testing Data/Standard Curve (for reference only) of CX43 elisa kit    CX43 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS729401. Click to request current manual
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NCBI/Uniprot data below describe general gene information for CX43. It may not necessarily be applicable to this product.
 NCBI GI #    29179440
 NCBI GeneID    30236
 NCBI Accession #    AAH49297.1 [Other Products]
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 UniProt Primary Accession #    O57474 [Other Products]
 UniProt Secondary Accession #    Q5TYN6; Q7ZTS1 [Other Products]
 UniProt Related Accession #    O57474 [Other Products]
 Molecular Weight    43,455 Da
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 NCBI Official Full Name    Cx43 protein
 NCBI Official Synonym Full Names    connexin 43
 NCBI Official Symbol    cx43 [Similar Products]
 NCBI Official Synonym Symbols   
gja1; cx43a1; zfCx43.3
[Similar Products]
 NCBI Protein Information    gap junction alpha-1 protein; shf; sof; connexin-43; etID309742.9; short fin protein; gap junction protein, alpha 1
 UniProt Protein Name    Gap junction alpha-1 protein
 UniProt Synonym Protein Names   
Connexin-43; Cx43; Short fin protein
 UniProt Gene Name    gja1 [Similar Products]
 UniProt Synonym Gene Names    cx43; shf; sof; Cx43 [Similar Products]
 UniProt Entry Name    CXA1_DANRE
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 UniProt Comments for CX43    Function: One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. Ref.1Plays a role in regulation of fin bone size and growth. Ref.1

Subunit structure: A connexon is composed of a hexamer of connexins.

Subcellular location: Cell membrane; Multi-pass membrane protein. Cell junction › gap junction.

Tissue specificity: Expressed in adult ovary, heart, lens and liver. In growing caudal fins, expressed in cells flanking the germinal region of newly growing segments and in osteoblasts at segment boundaries. Ref.1 Ref.2 Ref.5

Developmental stage: Expressed both maternally and zygotically. Zygotic expression is initiated before gastrulation and persists through to day 5 of development. Levels increase in late somitogenesis, and late embryonic and early larval stages. Embryonic expression is observed in many developing tissues and organs, including the notochord and brain, and heart and vasculature. Detected throughout the lens at 24 hours post-fertilization (hpf), but by 3-4 days development, restricted to the lateral epithelium and differentiating cells in the equatorial region. Ref.2 Ref.5

Miscellaneous: Morpholino knockdown of the protein results in heart folding defects, reduced hematopoiesis and small eyes.

Sequence similarities: Belongs to the connexin family. Alpha-type (group II) subfamily.
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 Research Articles on CX43    1. Data show that the cultured fibroblasts from patients with ossification of the posterior longitudinal ligament (OPLL) exhibited osteogenic characteristics, in which Cx43 played an important role.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with CX43 elisa kitDiseases associated with CX43 elisa kit
 Products by Pathway  Pathway Diagram
 Calcium Regulation In The Cardiac Cell Pathway antibodies  Calcium Regulation In The Cardiac Cell Pathway Diagram
 EGFR1 Signaling Pathway antibodies  EGFR1 Signaling Pathway Diagram
 Formation Of Annular Gap Junctions Pathway antibodies  Formation Of Annular Gap Junctions Pathway Diagram
 Gap Junction Pathway antibodies  Gap Junction Pathway Diagram
 Gap Junction Pathway antibodies  Gap Junction Pathway Diagram
 Gap Junction Assembly Pathway antibodies  Gap Junction Assembly Pathway Diagram
 Gap Junction Degradation Pathway antibodies  Gap Junction Degradation Pathway Diagram
 Gap Junction Trafficking Pathway antibodies  Gap Junction Trafficking Pathway Diagram
 Gap Junction Trafficking And Regulation Pathway antibodies  Gap Junction Trafficking And Regulation Pathway Diagram
 Membrane Trafficking Pathway antibodies  Membrane Trafficking Pathway Diagram
 Disease Name  Pubmed Publications
 Myocardial Ischemia Antibodies  >231 publications with CX43 and Myocardial Ischemia
 Death Antibodies  >206 publications with CX43 and Death
 Carcinoma Antibodies  >172 publications with CX43 and Carcinoma
 Congenital Abnormalities Antibodies  >141 publications with CX43 and Congenital Abnormalities
 Drug Toxicity Antibodies  >113 publications with CX43 and Drug Toxicity
 Fibrosis Antibodies  >97 publications with CX43 and Fibrosis
 Inflammation Antibodies  >90 publications with CX43 and Inflammation
 Adenocarcinoma Antibodies  >73 publications with CX43 and Adenocarcinoma
 Cardiomegaly Antibodies  >73 publications with CX43 and Cardiomegaly
 Hypertension Antibodies  >70 publications with CX43 and Hypertension
Organs/Tissues associated with CX43 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >1166 publications with CX43 and Muscle
 Brain Antibodies  >409 publications with CX43 and Brain
 Eye Antibodies  >272 publications with CX43 and Eye
 Bone Antibodies  >270 publications with CX43 and Bone
 Liver Antibodies  >240 publications with CX43 and Liver
 Reproductive System Antibodies  >36 publications with CX43 and Reproductive System
 Whole Body Antibodies  >8 publications with CX43 and Whole Body
 Fin Antibodies  >4 publications with CX43 and Fin
 Uncharacterized Tissue Antibodies  >1 publications with CX43 and Uncharacterized Tissue
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