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CDKN1A elisa kit :: Mouse Cyclin Dependent Kinase Inhibitor 1A (CDKN1A) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS451627
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $410 +1 FREE 8GB USB
96-Strip-Wells  /  $545 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,480 +3 FREE 8GB USB
10x96-Strip-Wells  /  $4,820 +6 FREE 8GB USB
 
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 Product Name   

Cyclin Dependent Kinase Inhibitor 1A (CDKN1A), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Cyclin Dependent Kinase Inhibitor 1A (CDKN1A) ELISA Kit

 Product Synonym Names    WAF1; p21; CIP1; p21WAF1; CAP20; CDKN1; MDA-6; SDI1; p21CIP1; CDK-Interacting Protein 1; Melanoma differentiation-associated protein 6
 Product Gene Name   

CDKN1A elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    L25610 mRNA
 3D Structure    ModBase 3D Structure for P38936
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 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of CDKN1A. No significant cross-reactivity or interference between CDKN1A and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
 Assay Type    Sandwich
 Detection Range    0.156-10ng/mL
 Sensitivity    0.066ng/mL
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CDKN1A were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
 Inter-assay Precision    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CDKN1A were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
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 Preparation and Storage    For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of CDKN1A elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for CDKN1A purchase    MBS451627 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Cyclin Dependent Kinase Inhibitor 1A (CDKN1A) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.156-10ng/mL in biological research samples containing CDKN1A, with an estimated sensitivity of 0.066ng/mL. The ELISA analytical biochemical technique of the MBS451627 kit is based on CDKN1A antibody-CDKN1A antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CDKN1A antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CDKN1A. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for CDKN1A elisa kit

   Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CDKN1A in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CDKN1A. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to CDKN1A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CDKN1A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of CDKN1A in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of CDKN1A elisa kit    CDKN1A elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS451627. Click to request current manual
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NCBI/Uniprot data below describe general gene information for CDKN1A. It may not necessarily be applicable to this product.
 NCBI GI #    11386203
 NCBI GeneID    1026
 NCBI Accession #    NP_000380.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_000389.4 [Other Products]
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 UniProt Primary Accession #    P38936 [Other Products]
 UniProt Secondary Accession #    Q14010; Q6FI05; Q9BUT4 [Other Products]
 UniProt Related Accession #    P38936 [Other Products]
 Molecular Weight    18,119 Da
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 NCBI Official Full Name    cyclin-dependent kinase inhibitor 1 isoform 1
 NCBI Official Synonym Full Names    cyclin dependent kinase inhibitor 1A
 NCBI Official Symbol    CDKN1A [Similar Products]
 NCBI Official Synonym Symbols   
P21; CIP1; SDI1; WAF1; CAP20; CDKN1; MDA-6; p21CIP1
[Similar Products]
 NCBI Protein Information    cyclin-dependent kinase inhibitor 1
 UniProt Protein Name    Cyclin-dependent kinase inhibitor 1
 UniProt Synonym Protein Names   
CDK-interacting protein 1; Melanoma differentiation-associated protein 6; MDA-6; p21
 UniProt Gene Name    CDKN1A [Similar Products]
 UniProt Synonym Gene Names    CAP20; CDKN1; CIP1; MDA6; PIC1; SDI1; WAF1; MDA-6 [Similar Products]
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 NCBI Summary for CDKN1A    This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-cyclin-dependent kinase2 or -cyclin-dependent kinase4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen, a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of cyclin-dependent kinase2, and may be instrumental in the execution of apoptosis following caspase activation. Mice that lack this gene have the ability to regenerate damaged or missing tissue. Multiple alternatively spliced variants have been found for this gene. [provided by RefSeq, Sep 2015]
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 UniProt Comments for CDKN1A    May be involved in p53/TP53 mediated inhibition of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex. Inhibits DNA synthesis by DNA polymerase delta by competing with POLD3 for PCNA binding (PubMed:11595739).
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 Research Articles on CDKN1A    1. Results show that while HOXB13 suppressed p21 expression via regulation of JNK signals, alteration of p21 expression also affected c-Jun and AP-1 activity in prostate cancer cells.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with CDKN1A elisa kitPathways associated with CDKN1A elisa kit
 Reference Product  PubMed Publications
 CDK2 elisa kit  >1289 publications with CDKN1A and CDK2
 CDK4 elisa kit  >831 publications with CDKN1A and CDK4
 TP53 elisa kit  >795 publications with CDKN1A and TP53
 PCNA elisa kit  >779 publications with CDKN1A and PCNA
 CCNB1 elisa kit  >355 publications with CDKN1A and CCNB1
 CCND1 elisa kit  >224 publications with CDKN1A and CCND1
 SKP2 elisa kit  >116 publications with CDKN1A and SKP2
 CCND3 elisa kit  >84 publications with CDKN1A and CCND3
 CCND2 elisa kit  >66 publications with CDKN1A and CCND2
 CCNE1 elisa kit  >44 publications with CDKN1A and CCNE1
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 ATM Signaling Pathway antibodies  ATM Signaling Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 Androgen Receptor Signaling Pathway antibodies  Androgen Receptor Signaling Pathway Diagram
 Androgen Receptor Signaling Pathway antibodies  Androgen Receptor Signaling Pathway Diagram
 Androgen Receptor Signaling Pathway antibodies  Androgen Receptor Signaling Pathway Diagram
 Angiopoietin Receptor Tie2-mediated Signaling Pathway antibodies  Angiopoietin Receptor Tie2-mediated Signaling Pathway Diagram
 Apoptosis-related Network Due To Altered Notch3 In Ovarian Cancer Pathway antibodies  Apoptosis-related Network Due To Altered Notch3 In Ovarian Cancer Pathway Diagram
Diseases associated with CDKN1A elisa kitOrgans/Tissues associated with CDKN1A elisa kit
 Disease Name  Pubmed Publications
 Adenocarcinoma Antibodies  >1087 publications with CDKN1A and Adenocarcinoma
 Breast Neoplasms Antibodies  >913 publications with CDKN1A and Breast Neoplasms
 Intestinal Neoplasms Antibodies  >730 publications with CDKN1A and Intestinal Neoplasms
 Cell Transformation, Neoplastic Antibodies  >525 publications with CDKN1A and Cell Transformation, Neoplastic
 Lung Neoplasms Antibodies  >521 publications with CDKN1A and Lung Neoplasms
 Neoplasms, Experimental Antibodies  >499 publications with CDKN1A and Neoplasms, Experimental
 Liver Diseases Antibodies  >483 publications with CDKN1A and Liver Diseases
 Liver Neoplasms Antibodies  >444 publications with CDKN1A and Liver Neoplasms
 Prostatic Neoplasms Antibodies  >416 publications with CDKN1A and Prostatic Neoplasms
 Colonic Neoplasms Antibodies  >396 publications with CDKN1A and Colonic Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >1011 publications with CDKN1A and Blood
 Lung Antibodies  >867 publications with CDKN1A and Lung
 Muscle Antibodies  >770 publications with CDKN1A and Muscle
 Liver Antibodies  >736 publications with CDKN1A and Liver
 Vascular Antibodies  >634 publications with CDKN1A and Vascular
 Prostate Antibodies  >508 publications with CDKN1A and Prostate
 Bone Antibodies  >453 publications with CDKN1A and Bone
 Skin Antibodies  >450 publications with CDKN1A and Skin
 Brain Antibodies  >419 publications with CDKN1A and Brain
 Kidney Antibodies  >369 publications with CDKN1A and Kidney
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