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CbS elisa kit :: Mouse Cystathionine Beta Synthase (CbS) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2021655 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $270 +1 FREE 8GB USB
48-Strip-Wells  /  $505 +1 FREE 8GB USB
96-Strip-Wells  /  $685 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,770 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,025 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Cystathionine Beta Synthase (CbS), ELISA Kit

 Also Known As   

Cystathionine Beta Synthase (CbS) ELISA Kit

 Product Synonym Names    HIP4; Beta-thionase; Serine sulfhydrase
 Product Gene Name   

CbS elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    236200
 3D Structure    ModBase 3D Structure for P35520
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 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Cystathionine Beta Synthase (CbS).
No significant cross-reactivity or interference between Cystathionine Beta Synthase (CbS) and analogues was observed.
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 Assay Type    Double-antibody Sandwich
 Samples    Tissue homogenates and Other Biological Fluids
 Detection Range    0.156-10ng/mL
 Sensitivity    < 0.059ng/mL
 Application    Enzyme-linked immunosorbent assay for Antigen Detection.
 Intra-assay Precision (Precision within an assay)    3 samples with low, middle and high level Cystathionine Beta Synthase (CbS) were tested 20 times on one plate, respectively.
 Inter-assay Precision (Precision between assays)    3 samples with low, middle and high level Cystathionine Beta Synthase (CbS) were tested on 3 different plates, 8 replicates in each plate.
 CV(%) =    SD/meanX100
 Intra-Assay    CV<10%
 Inter-Assay    CV<12%
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 Preparation and Storage    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 ISO Certification    Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
 Supply Chain Verification    Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available. Please inquire.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of CbS elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for CbS purchase    MBS2021655 is a ready-to-use microwell, strip plate Double-antibody Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Cystathionine Beta Synthase (CbS) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.156-10ng/mL in biological research samples containing CbS, with an estimated sensitivity of < 0.059ng/mL. The ELISA analytical biochemical technique of the MBS2021655 kit is based on CbS antibody-CbS antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CbS antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CbS. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Tissue homogenates and Other Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for CbS elisa kit

   The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cystathionine Beta Synthase (CbS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cystathionine Beta Synthase (CbS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cystathionine Beta Synthase (CbS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cystathionine Beta Synthase (CbS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Product Categories/Family for CbS elisa kit    Enzyme & Kinase
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 Typical Testing Data/Standard Curve (for reference only) of CbS elisa kit    CbS elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2021655. Click to request current manual
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NCBI/Uniprot data below describe general gene information for CbS. It may not necessarily be applicable to this product.
 NCBI GI #    30583147
 NCBI GeneID    875
 NCBI Accession #    AAP35818.1 [Other Products]
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 UniProt Primary Accession #    P35520 [Other Products]
 UniProt Secondary Accession #    Q99425; Q9BWC5; B2R993; D3DSK4 [Other Products]
 UniProt Related Accession #    P35520 [Other Products]
 Molecular Weight    61,863 Da
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 NCBI Official Full Name    cystathionine-beta-synthase
 NCBI Official Synonym Full Names    cystathionine-beta-synthase
 NCBI Official Symbol    CBS [Similar Products]
 NCBI Official Synonym Symbols   
HIP4
[Similar Products]
 NCBI Protein Information    cystathionine beta-synthase
 UniProt Protein Name    Cystathionine beta-synthase
 UniProt Synonym Protein Names   
Beta-thionase; Serine sulfhydrase
 Protein Family    Cystathionine beta-synthase
 UniProt Gene Name    CBS [Similar Products]
 UniProt Entry Name    CBS_HUMAN
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 NCBI Summary for CbS    The protein encoded by this gene acts as a homotetramer to catalyze the conversion of homocysteine to cystathionine, the first step in the transsulfuration pathway. The encoded protein is allosterically activated by adenosyl-methionine and uses pyridoxal phosphate as a cofactor. Defects in this gene can cause cystathionine beta-synthase deficiency (CBSD), which can lead to homocystinuria. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, May 2010]
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 UniProt Comments for CbS    CBS: Only known pyridoxal phosphate-dependent enzyme that contains heme. Important regulator of hydrogen sulfide, especially in the brain, utilizing cysteine instead of serine to catalyze the formation of hydrogen sulfide. Hydrogen sulfide is a gastratransmitter with signaling and cytoprotective effects such as acting as a neuromodulator in the brain to protect neurons against hypoxic injury. Defects in CBS are the cause of cystathionine beta- synthase deficiency (CBSD). CBSD is an enzymatic deficiency resulting in altered sulfur metabolism and homocystinuria. The clinical features of untreated homocystinuria due to CBS deficiency include myopia, ectopia lentis, mental retardation, skeletal anomalies resembling Marfan syndrome, and thromboembolic events. Light skin and hair can also be present. Biochemical features include increased urinary homocystine and methionine. Belongs to the cysteine synthase/cystathionine beta- synthase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Amino Acid Metabolism - cysteine and methionine; Amino Acid Metabolism - glycine, serine and threonine; EC 4.2.1.22; Lyase; Other Amino Acids Metabolism - selenoamino acid

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: intracellular membrane-bound organelle; nucleolus; cytosol; nucleus

Molecular Function: identical protein binding; protein binding; protein homodimerization activity; enzyme binding; cystathionine beta-synthase activity; metal ion binding; ubiquitin protein ligase binding; heme binding; pyridoxal phosphate binding

Biological Process: cysteine biosynthetic process via cystathionine; homocysteine catabolic process; sulfur amino acid metabolic process; L-serine metabolic process; transsulfuration; cysteine biosynthetic process from serine; homocysteine metabolic process; L-serine catabolic process; L-cysteine catabolic process

Disease: Homocystinuria Due To Cystathionine Beta-synthase Deficiency
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 Research Articles on CbS    1. CBS is distributed in the human fallopian tube epithelium.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with CbS elisa kitPathways associated with CbS elisa kit
 Reference Product  PubMed Publications
 MTR elisa kit  >80 publications with CbS and MTR
 BHMT elisa kit  >39 publications with CbS and BHMT
 SHMT1 elisa kit  >21 publications with CbS and SHMT1
 CTH elisa kit  >18 publications with CbS and CTH
 AHCY elisa kit  >9 publications with CbS and AHCY
 HTT elisa kit  >1 publications with CbS and HTT
 SHMT2 elisa kit  >1 publications with CbS and SHMT2
 Products by Pathway  Pathway Diagram
 Biosynthesis Of Amino Acids Pathway antibodies  Biosynthesis Of Amino Acids Pathway Diagram
 Biosynthesis Of Amino Acids Pathway antibodies  Biosynthesis Of Amino Acids Pathway Diagram
 Cysteine And Methionine Metabolism Pathway antibodies  Cysteine And Methionine Metabolism Pathway Diagram
 Cysteine And Methionine Metabolism Pathway antibodies  Cysteine And Methionine Metabolism Pathway Diagram
 Cysteine Biosynthesis, Homocysteine + Serine => Cysteine Pathway antibodies  Cysteine Biosynthesis, Homocysteine + Serine => Cysteine Pathway Diagram
 Cysteine Biosynthesis, Homocysteine + Serine => Cysteine Pathway antibodies  Cysteine Biosynthesis, Homocysteine + Serine => Cysteine Pathway Diagram
 Cysteine Formation From Homocysteine Pathway antibodies  Cysteine Formation From Homocysteine Pathway Diagram
 Folate-Alcohol And Cancer Pathway antibodies  Folate-Alcohol And Cancer Pathway Diagram
 Glycine, Serine And Threonine Metabolism Pathway antibodies  Glycine, Serine And Threonine Metabolism Pathway Diagram
 Glycine, Serine And Threonine Metabolism Pathway antibodies  Glycine, Serine And Threonine Metabolism Pathway Diagram
Diseases associated with CbS elisa kitOrgans/Tissues associated with CbS elisa kit
 Disease Name  Pubmed Publications
 Brain Diseases Antibodies  >497 publications with CbS and Brain Diseases
 Hyperhomocysteinemia Antibodies  >353 publications with CbS and Hyperhomocysteinemia
 Cardiovascular Diseases Antibodies  >348 publications with CbS and Cardiovascular Diseases
 Homocystinuria Antibodies  >187 publications with CbS and Homocystinuria
 Malnutrition Antibodies  >168 publications with CbS and Malnutrition
 Inflammation Antibodies  >108 publications with CbS and Inflammation
 Congenital Abnormalities Antibodies  >93 publications with CbS and Congenital Abnormalities
 Hypertension Antibodies  >70 publications with CbS and Hypertension
 Liver Diseases Antibodies  >66 publications with CbS and Liver Diseases
 Cognition Disorders Antibodies  >55 publications with CbS and Cognition Disorders
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >971 publications with CbS and Brain
 Blood Antibodies  >731 publications with CbS and Blood
 Vascular Antibodies  >299 publications with CbS and Vascular
 Liver Antibodies  >236 publications with CbS and Liver
 Muscle Antibodies  >185 publications with CbS and Muscle
 Nerve Antibodies  >184 publications with CbS and Nerve
 Eye Antibodies  >121 publications with CbS and Eye
 Kidney Antibodies  >110 publications with CbS and Kidney
 Ganglia Antibodies  >94 publications with CbS and Ganglia
 Lung Antibodies  >92 publications with CbS and Lung
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