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CST6 elisa kit :: Mouse Cystatin-M (CST6) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS7241348
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $455 +1 FREE 8GB USB
96-Strip-Wells  /  $660 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,490 +6 FREE 8GB USB
 
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 Product Name   

Cystatin-M (CST6), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Cystatin-M (CST6) ELISA Kit

 Product Gene Name   

CST6 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    601891
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 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of CST6. No significant cross-reactivity or interference between CST6 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CST6 and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Detection Range    1.0-25ng/mL
 Sensitivity    0.1ng/mL
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of CST6 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for CST6 purchase    MBS7241348 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Cystatin-M (CST6) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 1.0-25ng/mL in biological research samples containing CST6, with an estimated sensitivity of 0.1ng/mL. The ELISA analytical biochemical technique of the MBS7241348 kit is based on CST6 antibody-CST6 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CST6 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CST6. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for CST6 elisa kit

   Intended Uses: This CST6 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse CST6. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: CST6 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CST6 antibody and an CST6-HRP conjugate. The assay sample and buffer are incubated together with CST6-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CST6 concentration since CST6 from samples and CST6-HRP conjugate compete for the anti-CST6 antibody binding site. Since the number of sites is limited, as more sites are occupied by CST6 from the sample, fewer sites are left to bind CST6-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CST6 concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for CST6 elisa kit    Cell Biology
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 Typical Testing Data/Standard Curve (for reference only) of CST6 elisa kit    CST6 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS7241348. Click to request current manual
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NCBI/Uniprot data below describe general gene information for CST6. It may not necessarily be applicable to this product.
 NCBI GI #    4503113
 NCBI GeneID    1474
 NCBI Accession #    NP_001314.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001323.3 [Other Products]
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 UniProt Secondary Accession #    Q540N7 [Other Products]
 UniProt Related Accession #    Q15828 [Other Products]
 Molecular Weight    16,511 Da
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 NCBI Official Full Name    cystatin-M
 NCBI Official Synonym Full Names    cystatin E/M
 NCBI Official Symbol    CST6 [Similar Products]
 NCBI Protein Information    cystatin-M; cystatin 6; cystatin M; cystatin-6; cystatin-E; cystatin M/E; cysteine proteinase inhibitor
 UniProt Protein Name    Cystatin-M
 UniProt Synonym Protein Names   
Cystatin-6; Cystatin-E
 Protein Family    Cystatin
 UniProt Gene Name    CST6 [Similar Products]
 UniProt Entry Name    CYTM_HUMAN
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 NCBI Summary for CST6    The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions, where they appear to provide protective functions. This gene encodes a cystatin from the type 2 family, which is down-regulated in metastatic breast tumor cells as compared to primary tumor cells. Loss of expression is likely associated with the progression of a primary tumor to a metastatic phenotype. [provided by RefSeq, Jul 2008]
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 UniProt Comments for CST6    CST6: Shows moderate inhibition of cathepsin B but is not active against cathepsin C. Belongs to the cystatin family.

Protein type: Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: cornified envelope

Molecular Function: cysteine protease inhibitor activity

Biological Process: epidermis development; anatomical structure morphogenesis
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 Research Articles on CST6    1. Developed a Methylation-Sensitive High Resolution Melting Analysis (MS-HRMA) assay for the investigation of CST6 promoter methylation in breast cancer patients.CST6 promoter was methylated in 39/80 of FFPEs.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with CST6 elisa kitDiseases associated with CST6 elisa kit
 Reference Product  PubMed Publications
 CSTB elisa kit  >3 publications with CST6 and CSTB
 TGM3 elisa kit  >2 publications with CST6 and TGM3
 LGMN elisa kit  >2 publications with CST6 and LGMN
 CSTA elisa kit  >1 publications with CST6 and CSTA
 CTSS elisa kit  >1 publications with CST6 and CTSS
 LOR elisa kit  >1 publications with CST6 and LOR
 Disease Name  Pubmed Publications
 Breast Neoplasms Antibodies  >24 publications with CST6 and Breast Neoplasms
 Carcinoma Antibodies  >22 publications with CST6 and Carcinoma
 Brain Diseases Antibodies  >5 publications with CST6 and Brain Diseases
 Lung Neoplasms Antibodies  >4 publications with CST6 and Lung Neoplasms
 Inflammation Antibodies  >3 publications with CST6 and Inflammation
 Neoplasms, Experimental Antibodies  >3 publications with CST6 and Neoplasms, Experimental
 Carcinoma, Squamous Cell Antibodies  >3 publications with CST6 and Carcinoma, Squamous Cell
 Lung Diseases Antibodies  >3 publications with CST6 and Lung Diseases
 Liver Neoplasms Antibodies  >2 publications with CST6 and Liver Neoplasms
 Carcinoma, Hepatocellular Antibodies  >2 publications with CST6 and Carcinoma, Hepatocellular
Organs/Tissues associated with CST6 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Skin Antibodies  >13 publications with CST6 and Skin
 Lymph Node Antibodies  >5 publications with CST6 and Lymph Node
 Lung Antibodies  >4 publications with CST6 and Lung
 Uterus Antibodies  >4 publications with CST6 and Uterus
 Brain Antibodies  >4 publications with CST6 and Brain
 Thyroid Antibodies  >2 publications with CST6 and Thyroid
 Prostate Antibodies  >2 publications with CST6 and Prostate
 Pancreas Antibodies  >1 publications with CST6 and Pancreas
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