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DKK3 elisa kit :: Mouse DKK3 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2515630
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $410 +1 FREE 8GB USB
96-Strip-Wells  /  $490 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

DKK3, ELISA Kit

★Popular Item★
 Also Known As   

Mouse DKK3 (Dickkopf Related Protein 3) ELISA Kit

 Product Gene Name   

DKK3 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    605416
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 Species Reactivity    Mouse
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 Specificity    This kit recognizes natural and recombinantMouseDKK3. No significant cross-reactivity or interference between MouseDKK3 and analogues was observed.
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 Samples    Serum, Plasma, Biological Fluids
 Assay Type    Sandwich
 Detection Range    15.625-1000pg/mL
 Sensitivity    Min: 9.375pg/mL; Max: 1000pg/mL
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 Preparation and Storage    Store at 4 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of DKK3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for DKK3 purchase    MBS2515630 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the DKK3, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 15.625-1000pg/mL in biological research samples containing DKK3, with an estimated sensitivity of Min: 9.375pg/mL; Max: 1000pg/mL. The ELISA analytical biochemical technique of the MBS2515630 kit is based on DKK3 antibody-DKK3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect DKK3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, DKK3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for DKK3 elisa kit

   Intended Uses: This ELISA kit applies to the invitro quantitative determination of MouseDKK3concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to DKK3. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for DKK3and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain DKK3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of DKK3.You can calculate the concentration of DKK3in the samples by comparing the OD of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of DKK3 elisa kit    DKK3 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2515630. Click to request current manual
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NCBI/Uniprot data below describe general gene information for DKK3. It may not necessarily be applicable to this product.
 NCBI GI #    66346689
 NCBI GeneID    27122
 NCBI Accession #    NP_001018067.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001018057.1 [Other Products]
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 UniProt Secondary Accession #    Q9ULB7; A8K1I2; D3DQW1 [Other Products]
 UniProt Related Accession #    Q9UBP4 [Other Products]
 Molecular Weight    38,390 Da
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 NCBI Official Full Name    dickkopf-related protein 3
 NCBI Official Synonym Full Names    dickkopf WNT signaling pathway inhibitor 3
 NCBI Official Symbol    DKK3 [Similar Products]
 NCBI Official Synonym Symbols   
RIG; REIC
[Similar Products]
 NCBI Protein Information    dickkopf-related protein 3; RIG-like 5-6; RIG-like 7-1; dickkopf 3 homolog; dickkopf homolog 3; dickkopf-3; dkk-3; hDkk-3; regulated in glioma
 UniProt Protein Name    Dickkopf-related protein 3
 Protein Family    Dickkopf-related protein
 UniProt Gene Name    DKK3 [Similar Products]
 UniProt Synonym Gene Names    REIC; Dickkopf-3; Dkk-3; hDkk-3 [Similar Products]
 UniProt Entry Name    DKK3_HUMAN
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 NCBI Summary for DKK3    This gene encodes a protein that is a member of the dickkopf family. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. The expression of this gene is decreased in a variety of cancer cell lines and it may function as a tumor suppressor gene. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]
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 UniProt Comments for DKK3    DKK3: Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero- posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. Belongs to the dickkopf family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 11p15.2

Cellular Component: extracellular space

Biological Process: anatomical structure morphogenesis; Wnt receptor signaling pathway; adrenal gland development; negative regulation of aldosterone biosynthetic process; negative regulation of transcription, DNA-dependent
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 Research Articles on DKK3    1. a decrease in Dkk1 and Dkk3 mRNA and protein levels was observed in the ccRCC tissues. The serum Dkk1 and Dkk3 levels in the patients with ccRCC were found to be significantly lower than those in the controls.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with DKK3 elisa kitDiseases associated with DKK3 elisa kit
 Reference Product  PubMed Publications
 SFRP2 elisa kit  >31 publications with DKK3 and SFRP2
 WIF1 elisa kit  >30 publications with DKK3 and WIF1
 SFRP5 elisa kit  >25 publications with DKK3 and SFRP5
 LRP6 elisa kit  >9 publications with DKK3 and LRP6
 WNT6 elisa kit  >5 publications with DKK3 and WNT6
 LRP5 elisa kit  >5 publications with DKK3 and LRP5
 WNT2B elisa kit  >1 publications with DKK3 and WNT2B
 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >56 publications with DKK3 and Carcinoma
 Adenocarcinoma Antibodies  >30 publications with DKK3 and Adenocarcinoma
 Prostatic Neoplasms Antibodies  >19 publications with DKK3 and Prostatic Neoplasms
 Lung Neoplasms Antibodies  >17 publications with DKK3 and Lung Neoplasms
 Leukemia Antibodies  >12 publications with DKK3 and Leukemia
 Breast Neoplasms Antibodies  >11 publications with DKK3 and Breast Neoplasms
 Skin Diseases Antibodies  >11 publications with DKK3 and Skin Diseases
 Carcinoma, Hepatocellular Antibodies  >10 publications with DKK3 and Carcinoma, Hepatocellular
 Carcinoma, Non-Small-Cell Lung Antibodies  >9 publications with DKK3 and Carcinoma, Non-Small-Cell Lung
 Liver Neoplasms Antibodies  >8 publications with DKK3 and Liver Neoplasms
Organs/Tissues associated with DKK3 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Prostate Antibodies  >26 publications with DKK3 and Prostate
 Eye Antibodies  >25 publications with DKK3 and Eye
 Lung Antibodies  >22 publications with DKK3 and Lung
 Bone Antibodies  >22 publications with DKK3 and Bone
 Embryonic Tissue Antibodies  >15 publications with DKK3 and Embryonic Tissue
 Brain Antibodies  >15 publications with DKK3 and Brain
 Heart Antibodies  >11 publications with DKK3 and Heart
 Kidney Antibodies  >11 publications with DKK3 and Kidney
 Liver Antibodies  >10 publications with DKK3 and Liver
 Muscle Antibodies  >10 publications with DKK3 and Muscle
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