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Top1 elisa kit :: Mouse DNA topoisomerase 1 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2884099
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $430 +1 FREE 8GB USB
96-Strip-Wells  /  $570 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,515 +3 FREE 8GB USB
10x96-Strip-Wells  /  $4,520 +5 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

DNA topoisomerase 1 (Top1), ELISA Kit

 Also Known As   

Mouse DNA topoisomerase 1 ELISA Kit

 Product Synonym Names    DNA topoisomerase 1; DNA topoisomerase I; Top1; Top-1; 5.99.1.2
 Product Gene Name   

Top1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS2884099 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for Q04750
 Species Reactivity    Mouse
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 Specificity    Recombinant and natural Mouse DNA topoisomerase 1
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 Samples    Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids
 Detection Range    0.156-10 ng/mL
 Intra-assay Precision    Intra-Assay CV: <=6.2%
 Inter-assay Precision    Inter-Assay CV: <=8.3%
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 Preparation and Storage    For long term storage, please store the entire kit at -20 degree C.
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of Top1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for Top1 purchase    MBS2884099 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the DNA topoisomerase 1 (Top1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.156-10 ng/mL in biological research samples containing Top1. The ELISA analytical biochemical technique of the MBS2884099 kit is based on Top1 antibody-Top1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Top1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Top1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for Top1 elisa kit

   Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of Top1 elisa kit    Top1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2884099. Click to request current manual
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NCBI/Uniprot data below describe general gene information for Top1. It may not necessarily be applicable to this product.
 NCBI GI #    112734855
 NCBI GeneID    21969
 NCBI Accession #    NP_033434.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_009408.2 [Other Products]
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 UniProt Primary Accession #    Q04750 [Other Products]
 UniProt Secondary Accession #    A2A4B7 [Other Products]
 UniProt Related Accession #    Q04750 [Other Products]
 Molecular Weight    90,876 Da
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 NCBI Official Full Name    DNA topoisomerase 1
 NCBI Official Synonym Full Names    topoisomerase (DNA) I
 NCBI Official Symbol    Top1 [Similar Products]
 NCBI Official Synonym Symbols   
Top-1; AI467334; D130064I21Rik
[Similar Products]
 NCBI Protein Information    DNA topoisomerase 1
 UniProt Protein Name    DNA topoisomerase 1
 UniProt Synonym Protein Names   
DNA topoisomerase I
 Protein Family    DNA topoisomerase
 UniProt Gene Name    Top1 [Similar Products]
 UniProt Synonym Gene Names    Top-1 [Similar Products]
 UniProt Entry Name    TOP1_MOUSE
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 UniProt Comments for Top1    TOP1: Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. A chromosomal aberration involving TOP1 is found in a form of therapy-related myelodysplastic syndrome. Translocation t(11;20)(p15;q11) with NUP98. Belongs to the type IB topoisomerase family.

Protein type: Oncoprotein; EC 5.99.1.2; Nucleolus; Isomerase

Cellular Component: chromosome; cytoplasm; dense fibrillar component; fibrillar center; nuclear chromosome; nucleolus; nucleoplasm; nucleus; perikaryon

Molecular Function: chromatin binding; chromatin DNA binding; DNA binding; DNA topoisomerase (ATP-hydrolyzing) activity; DNA topoisomerase activity; DNA topoisomerase type I activity; isomerase activity; protein complex binding

Biological Process: chromatin remodeling; chromosome segregation; circadian regulation of gene expression; DNA replication; DNA topological change; embryonic cleavage; rhythmic process; rRNA transcription
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 Research Articles on Top1    1. Data indicate that mitochondrial topoisomerase I (TOP1mt) knockout (KO) mice showed a marked reduction in regeneration and hepatocyte proliferation.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with Top1 elisa kitPathways associated with Top1 elisa kit
 Reference Product  PubMed Publications
 Tdp1 elisa kit  >61 publications with Top1 and Tdp1
 Rad52 elisa kit  >12 publications with Top1 and Rad52
 Top2a elisa kit  >9 publications with Top1 and Top2a
 Mus81 elisa kit  >7 publications with Top1 and Mus81
 Trp53 elisa kit  >7 publications with Top1 and Trp53
 Top2b elisa kit  >4 publications with Top1 and Top2b
 Btbd2 elisa kit  >2 publications with Top1 and Btbd2
 Btbd1 elisa kit  >2 publications with Top1 and Btbd1
 Srsf1 elisa kit  >1 publications with Top1 and Srsf1
 Products by Pathway  Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 Post-translational Protein Modification Pathway antibodies  Post-translational Protein Modification Pathway Diagram
 SUMO E3 Ligases SUMOylate Target Proteins Pathway antibodies  SUMO E3 Ligases SUMOylate Target Proteins Pathway Diagram
 SUMOylation Pathway antibodies  SUMOylation Pathway Diagram
 SUMOylation Of DNA Replication Proteins Pathway antibodies  SUMOylation Of DNA Replication Proteins Pathway Diagram
Diseases associated with Top1 elisa kitOrgans/Tissues associated with Top1 elisa kit
 Disease Name  Pubmed Publications
 Leukemia Antibodies  >43 publications with Top1 and Leukemia
 Poisoning Antibodies  >30 publications with Top1 and Poisoning
 Breast Neoplasms Antibodies  >27 publications with Top1 and Breast Neoplasms
 Adenocarcinoma Antibodies  >21 publications with Top1 and Adenocarcinoma
 Nervous System Diseases Antibodies  >19 publications with Top1 and Nervous System Diseases
 Neoplasms, Experimental Antibodies  >18 publications with Top1 and Neoplasms, Experimental
 Brain Diseases Antibodies  >14 publications with Top1 and Brain Diseases
 Kidney Diseases Antibodies  >8 publications with Top1 and Kidney Diseases
 Prostatic Neoplasms Antibodies  >8 publications with Top1 and Prostatic Neoplasms
 Neoplasm Metastasis Antibodies  >8 publications with Top1 and Neoplasm Metastasis
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >41 publications with Top1 and Blood
 Lung Antibodies  >40 publications with Top1 and Lung
 Prostate Antibodies  >19 publications with Top1 and Prostate
 Brain Antibodies  >15 publications with Top1 and Brain
 Kidney Antibodies  >9 publications with Top1 and Kidney
 Liver Antibodies  >9 publications with Top1 and Liver
 Bone Marrow Antibodies  >8 publications with Top1 and Bone Marrow
 Skin Antibodies  >8 publications with Top1 and Skin
 Thymus Antibodies  >8 publications with Top1 and Thymus
 Ovary Antibodies  >5 publications with Top1 and Ovary
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