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DPD elisa kit :: Mouse DPD ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2503693
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $410 +1 FREE 8GB USB
96-Strip-Wells  /  $490 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

DPD, ELISA Kit

★Popular Item★
 Also Known As   

Mouse DPD (Deoxypyridinoline) ELISA Kit

 Product Gene Name   

DPD elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS2503693 COA    COA PDF
 3D Structure    ModBase 3D Structure for O89000
 Species Reactivity    Mouse
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 Specificity    This kit recognizes natural and some recombinant Mouse DPD. No significant crossreactivity or interference between Mouse DPD and analogues was observed.
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 Samples    Serum, plasma and other biological fluids
 Assay Type    Quantitative Competitive
 Detection Range    3.13-200 ng/mL
 Sensitivity    1.88 ng/mL
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 Preparation and Storage    Store at 4 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of DPD elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for DPD purchase    MBS2503693 is a ready-to-use microwell, strip plate Quantitative Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the DPD, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 3.13-200 ng/mL in biological research samples containing DPD, with an estimated sensitivity of 1.88 ng/mL. The ELISA analytical biochemical technique of the MBS2503693 kit is based on DPD antibody-DPD antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect DPD antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, DPD. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for DPD elisa kit

   Intended Uses: This ELISA kit can be applied to the in vitro quantitative determination of Mouse DPD concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Competitive-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse DPD. During the reaction, Mouse DPD in the sample or standard competes with a fixed amount of Mouse DPD on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Mouse DPD. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the Substrate Reagent is added to each well. The enzyme-substrate reaction is terminated by adding Stop Solution and the color change can be measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Mouse DPD in samples can be calculated by comparing the OD of the samples with the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of DPD elisa kit    DPD elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2503693. Click to request current manual
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NCBI/Uniprot data below describe general gene information for DPD. It may not necessarily be applicable to this product.
 NCBI GI #    13591940
 NCBI GeneID    81656
 NCBI Accession #    NP_112289.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_031027.1 [Other Products]
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 UniProt Primary Accession #    O89000 [Other Products]
 UniProt Related Accession #    O89000 [Other Products]
 Molecular Weight    111,468 Da
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 NCBI Official Full Name    dihydropyrimidine dehydrogenase
 NCBI Official Synonym Full Names    dihydropyrimidine dehydrogenase
 NCBI Official Symbol    Dpyd [Similar Products]
 NCBI Protein Information    dihydropyrimidine dehydrogenase [NADP(+)]; dihydropyrimidine dehydrogenase [NADP(+)]; DHPDHase; DPD; dihydrothymine dehydrogenase; dihydrouracil dehydrogenase
 UniProt Protein Name    Dihydropyrimidine dehydrogenase [NADP(+)]
 UniProt Synonym Protein Names   
Dihydrothymine dehydrogenase; Dihydrouracil dehydrogenase
 UniProt Gene Name    Dpyd [Similar Products]
 UniProt Synonym Gene Names    DPD; DHPDHase; DPD [Similar Products]
 UniProt Entry Name    DPYD_RAT
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 NCBI Summary for DPD    catalyzes the conversion of 5,6-dihydrouracil and NADP+ to uracil, NADPH, and H+ in pyrimidine degradation [RGD, Feb 2006]
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 UniProt Comments for DPD    DPYD: Involved in pyrimidine base degradation. Catalyzes the reduction of uracil and thymine. Also involved the degradation of the chemotherapeutic drug 5-fluorouracil. Homodimer. Found in most tissues with greatest activity found in liver and peripheral blood mononuclear cells. Belongs to the dihydropyrimidine dehydrogenase family.

Protein type: Other Amino Acids Metabolism - beta-alanine; Nucleotide Metabolism - pyrimidine; EC 1.3.1.2; Oxidoreductase; Cofactor and Vitamin Metabolism - pantothenate and CoA biosynthesis; Xenobiotic Metabolism - drug metabolism - other enzymes

Cellular Component: cytoplasm; cytosol

Molecular Function: protein homodimerization activity; uracil binding; dihydroorotate dehydrogenase activity; metal ion binding; 4 iron, 4 sulfur cluster binding; iron-sulfur cluster binding; dihydropyrimidine dehydrogenase (NADP+) activity; NADP binding

Biological Process: response to drug; uracil metabolic process; circadian rhythm; UMP biosynthetic process; uracil catabolic process; response to glucocorticoid stimulus; beta-alanine biosynthetic process; thymine catabolic process; purine base catabolic process; response to nutrient; thymidine catabolic process; pyrimidine base catabolic process
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with DPD elisa kitPathways associated with DPD elisa kit
 Reference Product  PubMed Publications
 Tymp elisa kit  >8 publications with DPD and Tymp
 Dpys elisa kit  >2 publications with DPD and Dpys
 Upp1 elisa kit  >1 publications with DPD and Upp1
 Umps elisa kit  >1 publications with DPD and Umps
 Products by Pathway  Pathway Diagram
 Drug Metabolism - Other Enzymes Pathway antibodies  Drug Metabolism - Other Enzymes Pathway Diagram
 Drug Metabolism - Other Enzymes Pathway antibodies  Drug Metabolism - Other Enzymes Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Pantothenate And CoA Biosynthesis Pathway antibodies  Pantothenate And CoA Biosynthesis Pathway Diagram
 Pantothenate And CoA Biosynthesis Pathway antibodies  Pantothenate And CoA Biosynthesis Pathway Diagram
 Pyrimidine Degradation, Uracil => Beta-alanine, Thymine => 3-aminoisobutanoate Pathway antibodies  Pyrimidine Degradation, Uracil => Beta-alanine, Thymine => 3-aminoisobutanoate Pathway Diagram
 Pyrimidine Degradation, Uracil => Beta-alanine, Thymine => 3-aminoisobutanoate Pathway antibodies  Pyrimidine Degradation, Uracil => Beta-alanine, Thymine => 3-aminoisobutanoate Pathway Diagram
 Pyrimidine Metabolism Pathway antibodies  Pyrimidine Metabolism Pathway Diagram
 Pyrimidine Metabolism Pathway antibodies  Pyrimidine Metabolism Pathway Diagram
 Beta-Alanine Metabolism Pathway antibodies  Beta-Alanine Metabolism Pathway Diagram
Diseases associated with DPD elisa kitOrgans/Tissues associated with DPD elisa kit
 Disease Name  Pubmed Publications
 Dihydropyrimidine Dehydrogenase Deficiency Antibodies  >74 publications with DPD and Dihydropyrimidine Dehydrogenase Deficiency
 Gastrointestinal Diseases Antibodies  >66 publications with DPD and Gastrointestinal Diseases
 Gastrointestinal Neoplasms Antibodies  >64 publications with DPD and Gastrointestinal Neoplasms
 Colorectal Neoplasms Antibodies  >55 publications with DPD and Colorectal Neoplasms
 Carcinoma Antibodies  >36 publications with DPD and Carcinoma
 Purine-Pyrimidine Metabolism, Inborn Errors Antibodies  >36 publications with DPD and Purine-Pyrimidine Metabolism, Inborn Errors
 Breast Neoplasms Antibodies  >21 publications with DPD and Breast Neoplasms
 Adenocarcinoma Antibodies  >19 publications with DPD and Adenocarcinoma
 Diarrhea Antibodies  >19 publications with DPD and Diarrhea
 Neutropenia Antibodies  >17 publications with DPD and Neutropenia
 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >16 publications with DPD and Liver
 Lung Antibodies  >8 publications with DPD and Lung
 Prostate Antibodies  >6 publications with DPD and Prostate
 Brain Antibodies  >5 publications with DPD and Brain
 Kidney Antibodies  >4 publications with DPD and Kidney
 Eye Antibodies  >2 publications with DPD and Eye
 Spleen Antibodies  >1 publications with DPD and Spleen
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