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OPRD1 elisa kit :: Mouse Delta-type opioid receptor (OPRD1) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS7234849
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $455 +1 FREE 8GB USB
96-Strip-Wells  /  $660 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,490 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Delta-type opioid receptor (OPRD1), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Delta-type opioid receptor (OPRD1) ELISA Kit

 Product Gene Name   

OPRD1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    165195
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 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of OPRD1. No significant cross-reactivity or interference between OPRD1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between OPRD1 and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
 Assay Type    Quantitative Competitive
 Sensitivity    0.1 ng/mL
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of OPRD1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for OPRD1 purchase    MBS7234849 is a ready-to-use microwell, strip plate Quantitative Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Delta-type opioid receptor (OPRD1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing OPRD1, with an estimated sensitivity of 0.1 ng/mL. The ELISA analytical biochemical technique of the MBS7234849 kit is based on OPRD1 antibody-OPRD1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect OPRD1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, OPRD1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for OPRD1 elisa kit

   Intended Uses: This OPRD1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse OPRD1.

Principle of the Assay: OPRD1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-OPRD1 antibody and an OPRD1-HRP conjugate. The assay sample and buffer are incubated together with OPRD1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the OPRD1 concentration since OPRD1 from samples and OPRD1-HRP conjugate compete for the anti-OPRD1 antibody binding site. Since the number of sites is limited, as more sites are occupied by OPRD1 from the sample, fewer sites are left to bind OPRD1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The OPRD1 concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for OPRD1 elisa kit    Neurobiology
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 Typical Testing Data/Standard Curve (for reference only) of OPRD1 elisa kit    OPRD1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS7234849. Click to request current manual
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NCBI/Uniprot data below describe general gene information for OPRD1. It may not necessarily be applicable to this product.
 NCBI GI #    63477962
 NCBI GeneID    4985
 NCBI Accession #    NP_000902.3 [Other Products]
 NCBI GenBank Nucleotide #    NM_000911.3 [Other Products]
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 UniProt Secondary Accession #    B5B0B8 [Other Products]
 UniProt Related Accession #    P41143 [Other Products]
 Molecular Weight    40,369 Da
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 NCBI Official Full Name    delta-type opioid receptor
 NCBI Official Synonym Full Names    opioid receptor, delta 1
 NCBI Official Symbol    OPRD1 [Similar Products]
 NCBI Official Synonym Symbols   
OPRD
[Similar Products]
 NCBI Protein Information    delta-type opioid receptor; DOR-1; D-OR-1; delta opioid receptor 1
 UniProt Protein Name    Delta-type opioid receptor
 Protein Family    Delta-type opioid receptor
 UniProt Gene Name    OPRD1 [Similar Products]
 UniProt Synonym Gene Names    OPRD; D-OR-1; DOR-1 [Similar Products]
 UniProt Entry Name    OPRD_HUMAN
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 UniProt Comments for OPRD1    DOR-1: delta opioid receptor, a G-protein coupled receptor. Inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. Highly stereoselective. Receptor for enkephalins.

Protein type: Receptor, GPCR; Membrane protein, multi-pass; Membrane protein, integral; GPCR, family 1

Chromosomal Location of Human Ortholog: 1p36.1-p34.3

Cellular Component: postsynaptic membrane; neuron projection; integral to plasma membrane; cytoplasm; plasma membrane; nerve terminal; intrinsic to plasma membrane; vesicle; lipid raft

Molecular Function: neuropeptide binding; protein binding; opioid receptor activity

Biological Process: adult locomotory behavior; activation of CREB transcription factor; regulation of calcium ion transport; sensory perception of pain; positive regulation of peptidyl-serine phosphorylation; G-protein signaling, coupled to cyclic nucleotide second messenger; negative regulation of protein oligomerization; synaptic transmission; G-protein coupled receptor protein signaling pathway; regulation of mitochondrial membrane potential; protein import into nucleus, translocation; neuropeptide signaling pathway; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); immune response; regulation of sensory perception of pain; G-protein signaling, adenylate cyclase inhibiting pathway
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 Research Articles on OPRD1    1. Allelic analysis was conducted on the relationship between opioid receptor-related genes and amoking behavior among Japanee men and women.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with OPRD1 elisa kitPathways associated with OPRD1 elisa kit
 Reference Product  PubMed Publications
 OPRM1 elisa kit  >32 publications with OPRD1 and OPRM1
 OPRK1 elisa kit  >22 publications with OPRD1 and OPRK1
 PENK elisa kit  >3 publications with OPRD1 and PENK
 Products by Pathway  Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 G Alpha (i) Signalling Events Pathway antibodies  G Alpha (i) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 GPCRs, Class A Rhodopsin-like Pathway antibodies  GPCRs, Class A Rhodopsin-like Pathway Diagram
 Neuroactive Ligand-receptor Interaction Pathway antibodies  Neuroactive Ligand-receptor Interaction Pathway Diagram
 Neuroactive Ligand-receptor Interaction Pathway antibodies  Neuroactive Ligand-receptor Interaction Pathway Diagram
 Peptide GPCRs Pathway antibodies  Peptide GPCRs Pathway Diagram
 Peptide Ligand-binding Receptors Pathway antibodies  Peptide Ligand-binding Receptors Pathway Diagram
 Signal Transduction Pathway antibodies  Signal Transduction Pathway Diagram
Diseases associated with OPRD1 elisa kitOrgans/Tissues associated with OPRD1 elisa kit
 Disease Name  Pubmed Publications
 Pain Antibodies  >14 publications with OPRD1 and Pain
 Substance-Related Disorders Antibodies  >14 publications with OPRD1 and Substance-Related Disorders
 Opioid-Related Disorders Antibodies  >13 publications with OPRD1 and Opioid-Related Disorders
 Nervous System Diseases Antibodies  >11 publications with OPRD1 and Nervous System Diseases
 Anorexia Antibodies  >4 publications with OPRD1 and Anorexia
 Cocaine-Related Disorders Antibodies  >2 publications with OPRD1 and Cocaine-Related Disorders
 Weight Loss Antibodies  >2 publications with OPRD1 and Weight Loss
 Psychoses, Substance-Induced Antibodies  >1 publications with OPRD1 and Psychoses, Substance-Induced
 Anxiety Disorders Antibodies  >1 publications with OPRD1 and Anxiety Disorders
 Poisoning Antibodies  >1 publications with OPRD1 and Poisoning
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >23 publications with OPRD1 and Brain
 Ovary Antibodies  >1 publications with OPRD1 and Ovary
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