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EL elisa kit :: Mouse EL ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2513362
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $410 +1 FREE 8GB USB
96-Strip-Wells  /  $490 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

EL, ELISA Kit

★Popular Item★
 Also Known As   

Mouse EL (Endothelial lipase) ELISA Kit

 Product Gene Name   

EL elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    603684
 3D Structure    ModBase 3D Structure for Q9Y5X9
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 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Mouse EL. No significant cross-reactivity or interference between Mouse EL and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Mouse EL and all the analogues, therefore, cross reaction may still exist.
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 Samples    Serum, Plasma, Biological Fluids
 Assay Type    Sandwich
 Detection Range    1.563-100ng/mL
 Sensitivity    Min: 0.938ng/mL; Max: 100ng/mL
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 Preparation and Storage    Store at 4 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of EL elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for EL purchase    MBS2513362 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the EL, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 1.563-100ng/mL in biological research samples containing EL, with an estimated sensitivity of Min: 0.938ng/mL; Max: 100ng/mL. The ELISA analytical biochemical technique of the MBS2513362 kit is based on EL antibody-EL antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect EL antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, EL. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for EL elisa kit

   Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of EL in Mouse serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse EL. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse EL and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse EL, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse EL. You can calculate the concentration of Mouse EL in the samples by comparing the OD of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of EL elisa kit    EL elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2513362. Click to request current manual
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NCBI/Uniprot data below describe general gene information for EL. It may not necessarily be applicable to this product.
 NCBI GI #    5174497
 NCBI GeneID    9388
 NCBI Accession #    NP_006024.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_006033.2 [Other Products]
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 UniProt Primary Accession #    Q9Y5X9 [Other Products]
 UniProt Secondary Accession #    Q6P9C8; Q6UW82; B0LPG6 [Other Products]
 UniProt Related Accession #    Q9Y5X9 [Other Products]
 Molecular Weight    39,362 Da
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 NCBI Official Full Name    endothelial lipase
 NCBI Official Synonym Full Names    lipase, endothelial
 NCBI Official Symbol    LIPG [Similar Products]
 NCBI Official Synonym Symbols   
EL; EDL; PRO719
[Similar Products]
 NCBI Protein Information    endothelial lipase; endothelial cell-derived lipase; lipoprotein lipase H
 UniProt Protein Name    Endothelial lipase
 UniProt Synonym Protein Names   
Endothelial cell-derived lipase; EDL; EL
 Protein Family    ELH
 UniProt Gene Name    LIPG [Similar Products]
 UniProt Synonym Gene Names    EDL; EL [Similar Products]
 UniProt Entry Name    LIPE_HUMAN
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 NCBI Summary for EL    The protein encoded by this gene has substantial phospholipase activity and may be involved in lipoprotein metabolism and vascular biology. This protein is designated a member of the TG lipase family by its sequence and characteristic lid region which provides substrate specificity for enzymes of the TG lipase family. [provided by RefSeq, Jul 2008]
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 UniProt Comments for EL    LIPG: Has phospholipase and triglyceride lipase activities. Hydrolyzes high density lipoproteins (HDL) more efficiently than other lipoproteins. Binds heparin. Belongs to the AB hydrolase superfamily. Lipase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Phospholipase; Secreted; Secreted, signal peptide; EC 3.1.1.3; Lipid Metabolism - glycerolipid

Chromosomal Location of Human Ortholog: 18q21.1

Cellular Component: Golgi apparatus; extracellular space; cell surface; early endosome

Molecular Function: heparin binding; lipoprotein lipase activity; phospholipase A1 activity; phospholipase activity

Biological Process: cell proliferation; cholesterol homeostasis; reverse cholesterol transport; phospholipid catabolic process; regulation of lipoprotein metabolic process; lipid metabolic process; positive regulation of cholesterol transport; response to nutrient; phospholipid homeostasis
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 Research Articles on EL    1. Genetic studies of patients with early onset of coronary artery disease (CAD) in the Chinese Han population show that the EL 584C/T variant is not always involved in the pathogenesis of early-onset CAD.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with EL elisa kitPathways associated with EL elisa kit
 Reference Product  PubMed Publications
 LPL elisa kit  >42 publications with EL and LPL
 LIPC elisa kit  >23 publications with EL and LIPC
 MGLL elisa kit  >2 publications with EL and MGLL
 Products by Pathway  Pathway Diagram
 Acylglycerol Degradation Pathway antibodies  Acylglycerol Degradation Pathway Diagram
 Acylglycerol Degradation Pathway antibodies  Acylglycerol Degradation Pathway Diagram
 Glycerolipid Metabolism Pathway antibodies  Glycerolipid Metabolism Pathway Diagram
 Glycerolipid Metabolism Pathway antibodies  Glycerolipid Metabolism Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Triacylglycerol Degradation Pathway antibodies  Triacylglycerol Degradation Pathway Diagram
Diseases associated with EL elisa kitOrgans/Tissues associated with EL elisa kit
 Disease Name  Pubmed Publications
 Atherosclerosis Antibodies  >56 publications with EL and Atherosclerosis
 Inflammation Antibodies  >25 publications with EL and Inflammation
 Dyslipidemias Antibodies  >18 publications with EL and Dyslipidemias
 Hyperlipidemias Antibodies  >13 publications with EL and Hyperlipidemias
 Hypertension Antibodies  >13 publications with EL and Hypertension
 Necrosis Antibodies  >10 publications with EL and Necrosis
 Fatty Liver Antibodies  >7 publications with EL and Fatty Liver
 Liver Diseases Antibodies  >7 publications with EL and Liver Diseases
 Insulin Resistance Antibodies  >7 publications with EL and Insulin Resistance
 Kidney Diseases Antibodies  >3 publications with EL and Kidney Diseases
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >10 publications with EL and Muscle
 Kidney Antibodies  >9 publications with EL and Kidney
 Placenta Antibodies  >8 publications with EL and Placenta
 Brain Antibodies  >6 publications with EL and Brain
 Testis Antibodies  >5 publications with EL and Testis
 Ovary Antibodies  >5 publications with EL and Ovary
 Embryonic Tissue Antibodies  >5 publications with EL and Embryonic Tissue
 Thyroid Antibodies  >2 publications with EL and Thyroid
 Pancreas Antibodies  >2 publications with EL and Pancreas
 Intestine Antibodies  >2 publications with EL and Intestine
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