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ELOA elisa kit :: Mouse ELOA ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2501104
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $410 +1 FREE 8GB USB
96-Strip-Wells  /  $490 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

ELOA, ELISA Kit

★Popular Item★
 Also Known As   

Mouse ELOA (Elongin A) ELISA Kit

 Product Gene Name   

ELOA elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 3D Structure    ModBase 3D Structure for Q9VCP0
 Species Reactivity    Mouse
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 Specificity    This kit recognizes natural and recombinant Mouse ELOA. No significant cross-reactivity or interference between Mouse ELOA and analogues was observed.
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 Samples    Serum, plasma and other biological fluids
 Assay Type    Quantitative Sandwich
 Detection Range    0.156-10ng/mL
 Sensitivity    0.094ng/mL
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 Preparation and Storage    Store at 4 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of ELOA elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for ELOA purchase    MBS2501104 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the ELOA, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.156-10ng/mL in biological research samples containing ELOA, with an estimated sensitivity of 0.094ng/mL. The ELISA analytical biochemical technique of the MBS2501104 kit is based on ELOA antibody-ELOA antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ELOA antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ELOA. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for ELOA elisa kit

   Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Mouse ELOA concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to ELOA. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for ELOA and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ELOA, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of ELOA. You can calculate the concentration of ELOA in the samples by comparing the OD of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of ELOA elisa kit    ELOA elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2501104. Click to request current manual
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NCBI/Uniprot data below describe general gene information for ELOA. It may not necessarily be applicable to this product.
 NCBI GI #    24649262
 NCBI GeneID    42749
 NCBI Accession #    NP_732846.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_170061.3 [Other Products]
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 UniProt Primary Accession #    Q9VCP0 [Other Products]
 UniProt Secondary Accession #    Q86NR0; Q95RH9 [Other Products]
 UniProt Related Accession #    Q9VCP0 [Other Products]
 Molecular Weight    62,482 Da
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 NCBI Official Full Name    elongin A, isoform A
 NCBI Official Synonym Full Names    Elongin A
 NCBI Official Symbol    EloA [Similar Products]
 NCBI Official Synonym Symbols   
CG6755; dEloA; DmelCG6755; Elo-A
[Similar Products]
 NCBI Protein Information    CG6755 gene product from transcript CG6755-RA; CG6755-PA; CG6755-PB; EloA-PA; EloA-PB; Elongin-A; ElonginA; elongin A
 UniProt Protein Name    Transcription elongation factor B polypeptide 3
 UniProt Synonym Protein Names   
Elongin-A; RNA polymerase II transcription factor SIII subunit A; dEloA
 Protein Family    Elongation of fatty acids protein
 UniProt Gene Name    EloA [Similar Products]
 UniProt Entry Name    ELOA1_DROME
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 UniProt Comments for ELOA    SIII, also known as elongin, is a general transcription elongation factor that increases the RNA polymerase II transcription elongation past template-encoded arresting sites. Subunit A is transcriptionally active and its transcription activity is strongly enhanced by binding to the dimeric complex of the SIII regulatory subunits B and C (elongin BC complex). May play an important role in metamorphosis.
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 Research Articles on ELOA    1. RNA polymerase II elongation factor, Elongin A, has an essential role in the regulation of heat shock gene expression
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with ELOA elisa kit
 Products by Pathway  Pathway Diagram
 Formation Of RNA Pol II Elongation Complex Pathway antibodies  Formation Of RNA Pol II Elongation Complex Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 RNA Polymerase II Pre-transcription Events Pathway antibodies  RNA Polymerase II Pre-transcription Events Pathway Diagram
 RNA Polymerase II Transcription Pathway antibodies  RNA Polymerase II Transcription Pathway Diagram
 RNA Polymerase II Transcription Elongation Pathway antibodies  RNA Polymerase II Transcription Elongation Pathway Diagram
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