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EPHB1 elisa kit :: Mouse EPH receptor B1 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS915405 EPHB1 elisa kit
Unit / Price
24-Strip-Wells (Limit 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

EPH receptor B1 (EPHB1), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Ephrin type-B receptor 1, EPHB1 ELISA Kit

 Product Synonym Names    Mouse Ephrin type-B receptor 1 (EPHB1) ELISA kit; ELK; EPHT2; FLJ37986; Hek6; NET; eph tyrosine kinase 2; ephrin receptor EphB1; soluble EPHB1 variant 1; EPH receptor B1
 Product Gene Name   

EPHB1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 3D Structure    ModBase 3D Structure for Q8CBF3
 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of mouse EPHB1. No significant cross-reactivity or interference between mouse EPHB1 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates
 Assay Type    Quantitative Sandwich
 Detection Range    0.312 ng/ml-20 ng/ml
 Sensitivity    <0.078 ng/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of EPHB1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for EPHB1 purchase    MBS915405 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the EPH receptor B1 (EPHB1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.312 ng/ml-20 ng/ml in biological research samples containing EPHB1, with an estimated sensitivity of <0.078 ng/ml. The ELISA analytical biochemical technique of the MBS915405 kit is based on EPHB1 antibody-EPHB1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect EPHB1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, EPHB1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for EPHB1 elisa kit

   Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for EPHB1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any EPHB1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EPHB1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EPHB1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Sample Manual Insert of MBS915405. Click to request current manual
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NCBI/Uniprot data below describe general gene information for EPHB1. It may not necessarily be applicable to this product.
 NCBI GI #    269973848
 NCBI GeneID    270190
 NCBI Accession #    NP_001161768.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001168296.1 [Other Products]
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 UniProt Primary Accession #    Q8CBF3 [Other Products]
 UniProt Secondary Accession #    Q3UY27; Q6PG23; Q8CBE2; B1B1C2 [Other Products]
 UniProt Related Accession #    Q8CBF3 [Other Products]
 Molecular Weight    109,881 Da
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 NCBI Official Full Name    ephrin type-B receptor 1 isoform 2
 NCBI Official Synonym Full Names    Eph receptor B1
 NCBI Official Symbol    Ephb1 [Similar Products]
 NCBI Official Synonym Symbols   
Elk; Net; Cek6; Elkh; Hek6; AW488255; 9330129L11; C130099E04Rik; ENSMUSG00000074119
[Similar Products]
 NCBI Protein Information    ephrin type-B receptor 1
 UniProt Protein Name    Ephrin type-B receptor 1
 Protein Family    Ephrin type-B receptor
 UniProt Gene Name    Ephb1 [Similar Products]
 UniProt Entry Name    EPHB1_MOUSE
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 UniProt Comments for EPHB1    EphB1: a receptor tyrosine kinase of the Eph family. Receptor for members of the ephrin-B family: ephrin-B1, -B2 and -B3. The Eph receptor tyrosine kinase family, the largest in the tyrosine kinase group, has fourteen members. They bind membrane-anchored ligands, ephrins, at sites of cell-cell contact, regulating the repulsion and adhesion of cells that underlie the establishment, maintenance, and remodeling of patterns of cellular organization. Eph signals are particularly important in regulating cell adhesion and cell migration during development, axon guidance, homeostasis and disease. EphA receptors bind to GPI-anchored ephrin-A ligands, while EphB receptors bind to ephrin-B proteins that have a transmembrane and cytoplasmic domain. Interactions between EphB receptor kinases and ephrin-B proteins transduce signals bidirectionally, signaling to both interacting cell types. Eph receptors and ephrins also regulate the adhesion of endothelial cells and are required for the remodeling of blood vessels. The ligand-activated form of EphB1 interacts with GRB2, GRB10 and NCK through their respective SH2 domains. Four alternatively spliced isoforms are known.

Protein type: EC 2.7.10.1; Kinase, protein; Membrane protein, integral; Protein kinase, TK; Protein kinase, tyrosine (receptor); TK group; Eph family

Cellular Component: filopodium tip; cell projection; membrane; early endosome membrane; integral to plasma membrane; axon; cytoplasm; plasma membrane; integral to membrane; endosome; lipid raft

Molecular Function: transmembrane-ephrin receptor activity; nucleotide binding; transmembrane receptor protein tyrosine kinase activity; protein kinase activity; transferase activity; protein binding; protein-tyrosine kinase activity; transferase activity, transferring phosphorus-containing groups; ephrin receptor activity; protein complex binding; kinase activity; axon guidance receptor activity; ATP binding

Biological Process: axon guidance; nervous system development; peptidyl-tyrosine phosphorylation; formation of immunological synapse; protein amino acid autophosphorylation; cranial nerve development; central nervous system projection neuron axonogenesis; optic nerve morphogenesis; regulation of JNK cascade; protein amino acid phosphorylation; cell-substrate adhesion; positive regulation of synaptogenesis; camera-type eye morphogenesis; neurogenesis; establishment of cell polarity; ephrin receptor signaling pathway; angiogenesis; cell adhesion; detection of temperature stimulus involved in sensory perception of pain; phosphorylation; transmembrane receptor protein tyrosine kinase signaling pathway; retinal ganglion cell axon guidance
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 Research Articles on EPHB1    1. EphB1 expression is activated in the spinal cord in a bone cancer model.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with EPHB1 elisa kitDiseases associated with EPHB1 elisa kit
 Products by Pathway  Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >18 publications with EPHB1 and Nervous System Diseases
 Brain Diseases Antibodies  >8 publications with EPHB1 and Brain Diseases
 Necrosis Antibodies  >4 publications with EPHB1 and Necrosis
 Disease Models, Animal Antibodies  >4 publications with EPHB1 and Disease Models, Animal
 Nerve Degeneration Antibodies  >2 publications with EPHB1 and Nerve Degeneration
 Movement Disorders Antibodies  >2 publications with EPHB1 and Movement Disorders
 Hematoma, Subdural, Acute Antibodies  >1 publications with EPHB1 and Hematoma, Subdural, Acute
 Hypertension Antibodies  >1 publications with EPHB1 and Hypertension
 Hyperkinesis Antibodies  >1 publications with EPHB1 and Hyperkinesis
Organs/Tissues associated with EPHB1 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >33 publications with EPHB1 and Brain
 Eye Antibodies  >23 publications with EPHB1 and Eye
 Embryonic Tissue Antibodies  >15 publications with EPHB1 and Embryonic Tissue
 Spinal Cord Antibodies  >14 publications with EPHB1 and Spinal Cord
 Inner Ear Antibodies  >4 publications with EPHB1 and Inner Ear
 Lung Antibodies  >4 publications with EPHB1 and Lung
 Mammary Gland Antibodies  >2 publications with EPHB1 and Mammary Gland
 Skin Antibodies  >1 publications with EPHB1 and Skin
 Testis Antibodies  >1 publications with EPHB1 and Testis
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