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Ecm1 elisa kit :: Mouse Extracellular matrix protein 1 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2886761 Ecm1 elisa kit
Unit / Price
48-Strip-Wells  /  $380 +1 FREE 8GB USB
96-Strip-Wells  /  $500 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,170 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,840 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Extracellular matrix protein 1 (Ecm1), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Extracellular matrix protein 1 ELISA Kit

 Product Synonym Names    Extracellular matrix protein 1; Secretory component p85; Ecm1
 Product Gene Name   

Ecm1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS2886761 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for Q61508
 Species Reactivity    Mouse
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 Assay Type    Sandwich
 Detection Range    0.312-20 ng/mL
 Sensitivity    0.159 ng/mL
 Intra-Assay CV    <=5.2%
 Inter-Assay CV    <=7.7%
 Recovery    85%
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 Preparation and Storage    For long term storage, please store the entire kit at -20 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of Ecm1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for Ecm1 purchase    MBS2886761 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Extracellular matrix protein 1 (Ecm1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.312-20 ng/mL in biological research samples containing Ecm1, with an estimated sensitivity of 0.159 ng/mL. The ELISA analytical biochemical technique of the MBS2886761 kit is based on Ecm1 antibody-Ecm1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Ecm1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Ecm1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for Ecm1 elisa kit

   Intended Uses||This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Sample Manual Insert of MBS2886761. Click to request current manual
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NCBI/Uniprot data below describe general gene information for Ecm1. It may not necessarily be applicable to this product.
 NCBI GI #    170295832
 NCBI GeneID    13601
 NCBI Accession #    NP_031925.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_007899.2 [Other Products]
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 UniProt Primary Accession #    Q61508 [Other Products]
 UniProt Secondary Accession #    Q3U5Q5 [Other Products]
 Molecular Weight    48,357 Da
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 NCBI Official Full Name    extracellular matrix protein 1 isoform 1
 UniProt Protein Name    Extracellular matrix protein 1
 UniProt Synonym Protein Names   
Secretory component p85
 Protein Family    Extracellular matrix protein
 UniProt Gene Name    Ecm1 [Similar Products]
 UniProt Entry Name    ECM1_MOUSE
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 UniProt Comments for Ecm1    ECM1: Involved in endochondral bone formation as negative regulator of bone mineralization. Stimulates the proliferation of endothelial cells and promotes angiogenesis. Inhibits MMP9 proteolytic activity. Defects in ECM1 are the cause of lipoid proteinosis (LiP); also known as lipoid proteinosis of Urbach and Wiethe or hyalinosis cutis et mucosae. LiP is a rare autosomal recessive disorder characterized by generalized thickening of skin, mucosae and certain viscera. Classical features include beaded eyelid papules and laryngeal infiltration leading to hoarseness. Histologically, there is widespread deposition of hyaline material and disruption/reduplication of basement membrane. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Cellular Component: extracellular matrix; extracellular region; extracellular space; proteinaceous extracellular matrix

Molecular Function: enzyme binding; interleukin-2 receptor binding; laminin binding; protease binding; protein C-terminus binding; signal transducer activity

Biological Process: angiogenesis; biomineral formation; inflammatory response; negative regulation of bone mineralization; negative regulation of cytokine and chemokine mediated signaling pathway; negative regulation of peptidase activity; ossification; positive regulation of angiogenesis; positive regulation of endothelial cell proliferation; positive regulation of I-kappaB kinase/NF-kappaB cascade; regulation of bone mineralization; regulation of T-helper 2 type immune response; regulation of transcription from RNA polymerase II promoter; signal transduction
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with Ecm1 elisa kitDiseases associated with Ecm1 elisa kit
 Reference Product  PubMed Publications
 Tfap2a elisa kit  >2 publications with Ecm1 and Tfap2a
 Tfap2c elisa kit  >2 publications with Ecm1 and Tfap2c
 Efemp1 elisa kit  >1 publications with Ecm1 and Efemp1
 Matn1 elisa kit  >1 publications with Ecm1 and Matn1
 Disease Name  Pubmed Publications
 Lipoid Proteinosis of Urbach and Wiethe Antibodies  >55 publications with Ecm1 and Lipoid Proteinosis of Urbach and Wiethe
 Carcinoma Antibodies  >26 publications with Ecm1 and Carcinoma
 Neoplasm Metastasis Antibodies  >15 publications with Ecm1 and Neoplasm Metastasis
 Breast Neoplasms Antibodies  >11 publications with Ecm1 and Breast Neoplasms
 Inflammation Antibodies  >10 publications with Ecm1 and Inflammation
 Colitis, Ulcerative Antibodies  >10 publications with Ecm1 and Colitis, Ulcerative
 Adenocarcinoma Antibodies  >9 publications with Ecm1 and Adenocarcinoma
 Carcinoma, Squamous Cell Antibodies  >8 publications with Ecm1 and Carcinoma, Squamous Cell
 Disease Models, Animal Antibodies  >3 publications with Ecm1 and Disease Models, Animal
 Kidney Diseases Antibodies  >2 publications with Ecm1 and Kidney Diseases
Organs/Tissues associated with Ecm1 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Skin Antibodies  >61 publications with Ecm1 and Skin
 Brain Antibodies  >10 publications with Ecm1 and Brain
 Bone Antibodies  >10 publications with Ecm1 and Bone
 Connective Tissue Antibodies  >7 publications with Ecm1 and Connective Tissue
 Lymph Node Antibodies  >7 publications with Ecm1 and Lymph Node
 Muscle Antibodies  >6 publications with Ecm1 and Muscle
 Lung Antibodies  >5 publications with Ecm1 and Lung
 Embryonic Tissue Antibodies  >5 publications with Ecm1 and Embryonic Tissue
 Kidney Antibodies  >5 publications with Ecm1 and Kidney
 Eye Antibodies  >5 publications with Ecm1 and Eye
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