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FPN elisa kit :: Mouse Ferroportin ELISA Kit

Scan QR to view Datasheet Catalog #    MBS755115
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $470 +1 FREE 8GB USB
96-Strip-Wells  /  $675 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,490 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Ferroportin (FPN), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Ferroportin ELISA Kit

 Product Gene Name   

FPN elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    gene 606069
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 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of FPN. No significant cross-reactivity or interference between FPN and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between FPN and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Detection Range    1.0-25ng/mL
 Sensitivity    0.1ng/mL
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of FPN elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for FPN purchase    MBS755115 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Ferroportin (FPN) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 1.0-25ng/mL in biological research samples containing FPN, with an estimated sensitivity of 0.1ng/mL. The ELISA analytical biochemical technique of the MBS755115 kit is based on FPN antibody-FPN antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect FPN antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, FPN. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for FPN elisa kit

   Intended Uses: This FPN ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse FPN. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: FPN ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-FPN antibody and an FPN-HRP conjugate. The assay sample and buffer are incubated together with FPN-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FPN concentration since FPN from samples and FPN-HRP conjugate compete for the anti-FPN antibody binding site. Since the number of sites is limited, as more sites are occupied by FPN from the sample, fewer sites are left to bind FPN-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FPN concentration in each sample is interpolated from this standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of FPN elisa kit    FPN elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS755115. Click to request current manual
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NCBI/Uniprot data below describe general gene information for FPN. It may not necessarily be applicable to this product.
 NCBI GI #    67633296
 NCBI GeneID    30061
 NCBI Accession #    AAY78556.1 [Other Products]
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 UniProt Secondary Accession #    Q6FI62; Q7Z4F8; Q8IVB2; Q9NRL0 [Other Products]
 UniProt Related Accession #    Q9NP59 [Other Products]
 Molecular Weight    62,542 Da
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 NCBI Official Full Name    ferroportin 1 variant I, partial
 NCBI Official Synonym Full Names    solute carrier family 40 (iron-regulated transporter), member 1
 NCBI Official Symbol    SLC40A1 [Similar Products]
 NCBI Official Synonym Symbols   
FPN1; HFE4; MTP1; IREG1; MST079; MSTP079; SLC11A3
[Similar Products]
 NCBI Protein Information    solute carrier family 40 member 1; iron regulated gene 1; putative ferroportin 1 variant IIIA; putative ferroportin 1 variant IIIB; solute carrier family 11 (proton-coupled divalent metal ion transporters), member 3
 UniProt Protein Name    Solute carrier family 40 member 1
 UniProt Synonym Protein Names   
Ferroportin-1; Iron-regulated transporter 1
 UniProt Gene Name    SLC40A1 [Similar Products]
 UniProt Synonym Gene Names    FPN1; IREG1; SLC11A3; MSTP079 [Similar Products]
 UniProt Entry Name    S40A1_HUMAN
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 NCBI Summary for FPN    The protein encoded by this gene is a cell membrane protein that may be involved in iron export from duodenal epithelial cells. Defects in this gene are a cause of hemochromatosis type 4 (HFE4). [provided by RefSeq, Jul 2008]
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 UniProt Comments for FPN    SLC40A1: May be involved in iron export from duodenal epithelial cell and also in transfer of iron between maternal and fetal circulation. Mediates iron efflux in the presence of a ferroxidase (hephaestin and/or ceruloplasmin). Defects in SLC40A1 are the cause of hemochromatosis type 4 (HFE4). HFE4 is an autosomal dominant iron-loading disorder characterized by early iron accumulation in reticuloendothelial cells and a marked increase in serum ferritin before elevation of the transferrin saturation. Belongs to the ferroportin (FP) (TC 2.A.100) family. SLC40A subfamily.

Protein type: Transporter, SLC family; Transporter; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 2q32

Cellular Component: multivesicular body; synaptic vesicle; integral to plasma membrane; basolateral plasma membrane; cytoplasm; plasma membrane; integral to membrane; intracellular

Molecular Function: protein binding; iron ion transmembrane transporter activity

Biological Process: anatomical structure morphogenesis; cellular iron ion homeostasis; positive regulation of transcription from RNA polymerase II promoter; lymphocyte homeostasis; transmembrane transport; negative regulation of apoptosis

Disease: Hemochromatosis, Type 4
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 Research Articles on FPN    1. Tryptophan residue 42, which is localized within the extracellular end of the ferroportin pore, is likely involved in the iron export function of SLC40A1.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with FPN elisa kitPathways associated with FPN elisa kit
 Reference Product  PubMed Publications
 HFE elisa kit  >51 publications with FPN and HFE
 HAMP elisa kit  >42 publications with FPN and HAMP
 TFR2 elisa kit  >35 publications with FPN and TFR2
 HFE2 elisa kit  >10 publications with FPN and HFE2
 SLC11A2 elisa kit  >6 publications with FPN and SLC11A2
 HEPH elisa kit  >1 publications with FPN and HEPH
 Products by Pathway  Pathway Diagram
 Iron Metabolism In Placenta Pathway antibodies  Iron Metabolism In Placenta Pathway Diagram
 Iron Uptake And Transport Pathway antibodies  Iron Uptake And Transport Pathway Diagram
 Metal Ion SLC Transporters Pathway antibodies  Metal Ion SLC Transporters Pathway Diagram
 Mineral Absorption Pathway antibodies  Mineral Absorption Pathway Diagram
 Mineral Absorption Pathway antibodies  Mineral Absorption Pathway Diagram
 SLC-mediated Transmembrane Transport Pathway antibodies  SLC-mediated Transmembrane Transport Pathway Diagram
 Transmembrane Transport Of Small Molecules Pathway antibodies  Transmembrane Transport Of Small Molecules Pathway Diagram
 Transport Of Glucose And Other Sugars, Bile Salts And Organic Acids, Metal Ions And Amine Compounds Pathway antibodies  Transport Of Glucose And Other Sugars, Bile Salts And Organic Acids, Metal Ions And Amine Compounds Pathway Diagram
Diseases associated with FPN elisa kitOrgans/Tissues associated with FPN elisa kit
 Disease Name  Pubmed Publications
 Iron Overload Antibodies  >68 publications with FPN and Iron Overload
 Hemochromatosis Antibodies  >59 publications with FPN and Hemochromatosis
 Anemia Antibodies  >16 publications with FPN and Anemia
 Liver Diseases Antibodies  >15 publications with FPN and Liver Diseases
 Inflammation Antibodies  >6 publications with FPN and Inflammation
 Fibrosis Antibodies  >4 publications with FPN and Fibrosis
 Brain Diseases Antibodies  >3 publications with FPN and Brain Diseases
 Breast Neoplasms Antibodies  >3 publications with FPN and Breast Neoplasms
 Fatty Liver Antibodies  >2 publications with FPN and Fatty Liver
 Cardiovascular Diseases Antibodies  >2 publications with FPN and Cardiovascular Diseases
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >44 publications with FPN and Blood
 Liver Antibodies  >36 publications with FPN and Liver
 Heart Antibodies  >6 publications with FPN and Heart
 Spleen Antibodies  >6 publications with FPN and Spleen
 Bone Antibodies  >6 publications with FPN and Bone
 Intestine Antibodies  >6 publications with FPN and Intestine
 Kidney Antibodies  >6 publications with FPN and Kidney
 Muscle Antibodies  >5 publications with FPN and Muscle
 Brain Antibodies  >4 publications with FPN and Brain
 Lung Antibodies  >3 publications with FPN and Lung
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